cDNA Clone MYPT2

• Plasmid: pCMV-FLAG MYPT2
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-MYPT2
• Genbank: NM_002481.2
• Tag: FLAG

Sequence of Insert

GGATCCATGGCGGAACTGGAGCACCTAGGAGGGAAGCGGGCAGAGTCGGCGCGAATGCGGCGGGCAGAGCAGCTTCGGCGCTGGCGGGGCTCGCTGACAGAGCAGGAGCCTGCGGAGCGACGAGGCGCGGGGCGGCAGCCGCTGACCAGGCGCGGGAGCCCCAGGGTCCGCTTCGAGGACGGTGCTGTCTTTCTGGCCGCCTGCTCTAGCGGGGACACCGACGAGGTGAGAAAGCTTCTGGCAAGAGGTGCTGATATCAACACGGTCAACGTGGACGGCTTGACAGCCCTGCACCAGGCATGTATTGATGAAAATTTGGACATGGTGAAGTTTCTGGTGGAGAACAGAGCCAATGTAAACCAGCAAGACAACGAGGGCTGGACACCCCTTCATGCAGCAGCTTCCTGTGGCTATCTCAACATAGCAGAGTATTTCATTAATCACGGAGCCAGTGTAGGTATTGTCAATAGTGAAGGTGAAGTTCCCTCTGACCTTGCAGAAGAGCCAGCCATGAAGGATCTTCTTCTGGAGCAAGTAAAGAAGCAAGGAGTTGATCTAGAGCAGTCAAGAAAAGAAGAAGAGCAGCAGATGTTGCAGGATGCCCGCCAGTGGCTCAACAGTGGGAAAATAGAGGATGTGAGGCAGGCTCGCTCAGGGGCTACAGCCCTTCATGTGGCTGCTGCCAAGGGCTACTCTGAAGTCCTCAGACTTTTAATTCAGGCTGGCTATGAACTCAATGTTCAGGATTATGATGGCTGGACTCCCCTCCATGCTGCTGCACACTGGGGAGTGAAGGAGGCTTGCTCCATCCTGGCAGAAGCACTTTGTGACATGGATATTCGAAATAAACTGGGCCAGACACCATTTGATGTGGCTGATGAGGGTCTCGTGGAGCATTTGGAGTTGCTCCAGAAGAAGCAGAATGTGCTTCGAAGTGAAAAGGAGACACGGAACAAACTCATTGAGTCAGATCTGAACAGCAAGATTCAGAGTGGGTTCTTTAAGAACAAAGAGAAGATGCTCTATGAGGAGGAGACACCTAAGTCCCAAGAAATGGAGGAAGAAAATAAAGAATCTAGTAGCTCCAGCTCAGAGGAGGAGGAAGGTGAAGATGAAGCTTCTGAGTCAGAAACTGAGAAGGAGGCAGATAAAAAGCCAGAAGCCTTTGTCAATCATTCCAACTCTGAAAGCAAGAGTAGTATCACAGAGCAGATACCAGCACCAGCTCAAAATACCTTCTCTGCCTCTTCTGCTAGGAGGTTCTCTTCTGGCCTTTTTAACAAGCCAGAAGAGCCCAAAGATGAATCTCCTTCTTCATGGAGATTGGGACTGAGAAAAACTGGCAGCCACAACATGCTGAGTGAGGTGGCCAATTCCAGGGAACCTATAAGGGACCGAGGCTCTTCCATCTATCGCTCCTCTTCAAGCCCTCGGATTTCTGCTCTACTGGACAACAAAGATAAGGAGAGAGAAAACAAAAGCTATATTAGTTCACTAGCACCCCGGAAGCTCAACAGCACAAGTGATATTGAAGAAAAGGAGAACAGAGAATCAGCTGTTAATCTAGTGAGGAGTGGCTCCTATACCCGGCAGCTATGGAGGGATGAGGCAAAAGGAAATGAAATCCCACAGACAATTGCTCCTTCCACCTATGTATCAACTTACTTGAAAAGGACTCCTCACAAATCCCAGGCCGACACAACAGCAGAGAAAACAGCAGACAATGTCTCTTCTAGCACCCCGCTCTGTGTGATCACCAATCGCCCTCTTCCTAGCACTGCCAATGGGGTTACAGCTACTCCTGTGCTCTCCATTACTGGAACAGATTCCTCTGTGGAAGCCAGGGAGAAGAGGAGGTCCTATCTGACTCCTGTACGGGATGAGGAAGCAGAGTCTTTACGGAAAGCACGCTCCAGACAAGCTCGGCAGACACGAAGGTCTACTCAAGGTGTCACCCTAACAGACCTTCAAGAAGCAGAAAGGACATTCAGCCGGTCGAGGGCAGAGAGGCAAGCTCAGGAGCAGCCTCGTGAGAAGCCCACAGACACTGAAGGGCTTGAGGGGAGCCCTGAGAAGCATGAGCCCTCAGCAGTTCCAGCAACAGAAGCTGGGGAGGGCCAGCAGCCCTGGGGCAGGAGTCTGGATGAAGAGCCTATCTGTCATCGCCTGAGGTGCCCAGCTCAGCCAGACAAACCCACCACGCCAGCATCTCCTTCTACGTCAAGACCCTCACTCTACACCAGTTCCCACCTGCTATGGACAAATAGATTTTCAGTCCCTGATTCTGAGAGTTCAGAGACTACCACAAACACTACAACTGCAAAGGAAATGGACAAAAATGAGAATGAAGAAGCAGATTTGGATGAGCAGTCCTCTAAGAGGCTGTCCATCCGAGAGAGGAGGCGGCCCAAGGAACGACGAAGAGGCACAGGCATCAATTTCTGGACAAAGGATGAGGATGAAACTGATGGCTCTGAAGAGGTCAAAGAAACGTGGCATGAAAGACTTTCTAGGTTGGAATCGGGAGGTAGTAATCCTACAACCAGTGATTCTTACGGTGACCGGGCTTCAGCAAGAGCCCGTCGGGAGGCCCGGGAGGCCCGCCTAGCCACCCTGACCAGCCGTGTAGAAGAAGACAGCAACAGAGATTATAAAAAACTCTATGAGAGTGCTCTGACTGAAAACCAAAAACTGAAAACAAAACTTCAGGAAGCCCAGCTAGAGCTAGCAGATATAAAGTCCAAGCTTGAGAAGGTGGCCCAGAAACAAGAAAAGACCTCTGACCGATCATCAGTGCTGGAGATGGAGAAACGGGAGAGGCGAGCCTTGGAGCGCAAAATGTCAGAAATGGAGGAAGAAATGAAGGTGTTAACAGAACTGAAATCCGACAACCAGAGGCTGAAAGATGAAAATGGTGCCCTCATCAGAGTCATCAGCAAACTGTCCAAGTAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMAELEHLGGKRAESARMRRAEQLRRWRGSLTEQEPAERRGAGRQPLTRRGSPRVRFEDGAVFLAACSSGDTDEVRKLLARGADINTVNVDGLTALHQACIDENLDMVKFLVENRANVNQQDNEGWTPLHAAASCGYLNIAEYFINHGASVGIVNSEGEVPSDLAEEPAMKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGKIEDVRQARSGATALHVAAAKGYSEVLRLLIQAGYELNVQDYDGWTPLHAAAHWGVKEACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLELLQKKQNVLRSEKETRNKLIESDLNSKIQSGFFKNKEKMLYEEETPKSQEMEEENKESSSSSSEEEEGEDEASESETEKEADKKPEAFVNHSNSESKSSITEQIPAPAQNTFSASSARRFSSGLFNKPEEPKDESPSSWRLGLRKTGSHNMLSEVANSREPIRDRGSSIYRSSSSPRISALLDNKDKERENKSYISSLAPRKLNSTSDIEEKENRESAVNLVRSGSYTRQLWRDEAKGNEIPQTIAPSTYVSTYLKRTPHKSQADTTAEKTADNVSSSTPLCVITNRPLPSTANGVTATPVLSITGTDSSVEAREKRRSYLTPVRDEEAESLRKARSRQARQTRRSTQGVTLTDLQEAERTFSRSRAERQAQEQPREKPTDTEGLEGSPEKHEPSAVPATEAGEGQQPWGRSLDEEPICHRLRCPAQPDKPTTPASPSTSRPSLYTSSHLLWTNRFSVPDSESSETTTNTTTAKEMDKNENEEADLDEQSSKRLSIRERRRPKERRRGTGINFWTKDEDETDGSEEVKETWHERLSRLESGGSNPTTSDSYGDRASARARREAREARLATLTSRVEEDSNRDYKKLYESALTENQKLKTKLQEAQLELADIKSKLEKVAQKQEKTSDRSSVLEMEKRERRALERKMSEMEEEMKVLTELKSDNQRLKDENGALIRVISKLSK*

• Vector Type: Mammalian
• DU Number: DU30286
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: I182/V (like in MYPT1); AND Q919 missing from our variant