cDNA Clone
MYPT2

Synonyms
MGC131980; MGC87886; PPP1R12B
Plasmid
pEBG6P-MYPT2
Parent Plasmid
pEBG6P
Expressed
GST-MYPT2
Genbank
NM_002481.2 GI:14195595 with changes - see notes
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGGCGGAACTGGAGCACCTAGGAGGGAAGCGGGCAGAGTCGGCGCGAATGCGGCGGGCAGAGCAGCTTCGGCGCTGGCGGGGCTCGCTGACAGAGCAGGAGCCTGCGGAGCGACGAGGCGCGGGGCGGCAGCCGCTGACCAGGCGCGGGAGCCCCAGGGTCCGCTTCGAGGACGGTGCTGTCTTTCTGGCCGCCTGCTCTAGCGGGGACACCGACGAGGTGAGAAAGCTTCTGGCAAGAGGTGCTGATATCAACACGGTCAACGTGGACGGCTTGACAGCCCTGCACCAGGCATGTATTGATGAAAATTTGGACATGGTGAAGTTTCTGGTGGAGAACAGAGCCAATGTAAACCAGCAAGACAACGAGGGCTGGACACCCCTTCATGCAGCAGCTTCCTGTGGCTATCTCAACATAGCAGAGTATTTCATTAATCACGGAGCCAGTGTAGGTATTGTCAATAGTGAAGGTGAAGTTCCCTCTGACCTTGCAGAAGAGCCAGCCATGAAGGATCTTCTTCTGGAGCAAGTAAAGAAGCAAGGAGTTGATCTAGAGCAGTCAAGAAAAGAAGAAGAGCAGCAGATGTTGCAGGATGCCCGCCAGTGGCTCAACAGTGGGAAAATAGAGGATGTGAGGCAGGCTCGCTCAGGGGCTACAGCCCTTCATGTGGCTGCTGCCAAGGGCTACTCTGAAGTCCTCAGACTTTTAATTCAGGCTGGCTATGAACTCAATGTTCAGGATTATGATGGCTGGACTCCCCTCCATGCTGCTGCACACTGGGGAGTGAAGGAGGCTTGCTCCATCCTGGCAGAAGCACTTTGTGACATGGATATTCGAAATAAACTGGGCCAGACACCATTTGATGTGGCTGATGAGGGTCTCGTGGAGCATTTGGAGTTGCTCCAGAAGAAGCAGAATGTGCTTCGAAGTGAAAAGGAGACACGGAACAAACTCATTGAGTCAGATCTGAACAGCAAGATTCAGAGTGGGTTCTTTAAGAACAAAGAGAAGATGCTCTATGAGGAGGAGACACCTAAGTCCCAAGAAATGGAGGAAGAAAATAAAGAATCTAGTAGCTCCAGCTCAGAGGAGGAGGAAGGTGAAGATGAAGCTTCTGAGTCAGAAACTGAGAAGGAGGCAGATAAAAAGCCAGAAGCCTTTGTCAATCATTCCAACTCTGAAAGCAAGAGTAGTATCACAGAGCAGATACCAGCACCAGCTCAAAATACCTTCTCTGCCTCTTCTGCTAGGAGGTTCTCTTCTGGCCTTTTTAACAAGCCAGAAGAGCCCAAAGATGAATCTCCTTCTTCATGGAGATTGGGACTGAGAAAAACTGGCAGCCACAACATGCTGAGTGAGGTGGCCAATTCCAGGGAACCTATAAGGGACCGAGGCTCTTCCATCTATCGCTCCTCTTCAAGCCCTCGGATTTCTGCTCTACTGGACAACAAAGATAAGGAGAGAGAAAACAAAAGCTATATTAGTTCACTAGCACCCCGGAAGCTCAACAGCACAAGTGATATTGAAGAAAAGGAGAACAGAGAATCAGCTGTTAATCTAGTGAGGAGTGGCTCCTATACCCGGCAGCTATGGAGGGATGAGGCAAAAGGAAATGAAATCCCACAGACAATTGCTCCTTCCACCTATGTATCAACTTACTTGAAAAGGACTCCTCACAAATCCCAGGCCGACACAACAGCAGAGAAAACAGCAGACAATGTCTCTTCTAGCACCCCGCTCTGTGTGATCACCAATCGCCCTCTTCCTAGCACTGCCAATGGGGTTACAGCTACTCCTGTGCTCTCCATTACTGGAACAGATTCCTCTGTGGAAGCCAGGGAGAAGAGGAGGTCCTATCTGACTCCTGTACGGGATGAGGAAGCAGAGTCTTTACGGAAAGCACGCTCCAGACAAGCTCGGCAGACACGAAGGTCTACTCAAGGTGTCACCCTAACAGACCTTCAAGAAGCAGAAAGGACATTCAGCCGGTCGAGGGCAGAGAGGCAAGCTCAGGAGCAGCCTCGTGAGAAGCCCACAGACACTGAAGGGCTTGAGGGGAGCCCTGAGAAGCATGAGCCCTCAGCAGTTCCAGCAACAGAAGCTGGGGAGGGCCAGCAGCCCTGGGGCAGGAGTCTGGATGAAGAGCCTATCTGTCATCGCCTGAGGTGCCCAGCTCAGCCAGACAAACCCACCACGCCAGCATCTCCTTCTACGTCAAGACCCTCACTCTACACCAGTTCCCACCTGCTATGGACAAATAGATTTTCAGTCCCTGATTCTGAGAGTTCAGAGACTACCACAAACACTACAACTGCAAAGGAAATGGACAAAAATGAGAATGAAGAAGCAGATTTGGATGAGCAGTCCTCTAAGAGGCTGTCCATCCGAGAGAGGAGGCGGCCCAAGGAACGACGAAGAGGCACAGGCATCAATTTCTGGACAAAGGATGAGGATGAAACTGATGGCTCTGAAGAGGTCAAAGAAACGTGGCATGAAAGACTTTCTAGGTTGGAATCGGGAGGTAGTAATCCTACAACCAGTGATTCTTACGGTGACCGGGCTTCAGCAAGAGCCCGTCGGGAGGCCCGGGAGGCCCGCCTAGCCACCCTGACCAGCCGTGTAGAAGAAGACAGCAACAGAGATTATAAAAAACTCTATGAGAGTGCTCTGACTGAAAACCAAAAACTGAAAACAAAACTTCAGGAAGCCCAGCTAGAGCTAGCAGATATAAAGTCCAAGCTTGAGAAGGTGGCCCAGAAACAAGAAAAGACCTCTGACCGATCATCAGTGCTGGAGATGGAGAAACGGGAGAGGCGAGCCTTGGAGCGCAAAATGTCAGAAATGGAGGAAGAAATGAAGGTGTTAACAGAACTGAAATCCGACAACCAGAGGCTGAAAGATGAAAATGGTGCCCTCATCAGAGTCATCAGCAAACTGTCCAAGTAGCGGCCGC

Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAELEHLGGKRAE
SARMRRAEQLRRWRGSLTEQEPAERRGAGRQPLTRRGSPRVRFEDGAVFL
AACSSGDTDEVRKLLARGADINTVNVDGLTALHQACIDENLDMVKFLVEN
RANVNQQDNEGWTPLHAAASCGYLNIAEYFINHGASVGIVNSEGEVPSDL
AEEPAMKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGKIEDVRQ
ARSGATALHVAAAKGYSEVLRLLIQAGYELNVQDYDGWTPLHAAAHWGVK
EACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLELLQKKQNVLRSEKE
TRNKLIESDLNSKIQSGFFKNKEKMLYEEETPKSQEMEEENKESSSSSSE
EEEGEDEASESETEKEADKKPEAFVNHSNSESKSSITEQIPAPAQNTFSA
SSARRFSSGLFNKPEEPKDESPSSWRLGLRKTGSHNMLSEVANSREPIRD
RGSSIYRSSSSPRISALLDNKDKERENKSYISSLAPRKLNSTSDIEEKEN
RESAVNLVRSGSYTRQLWRDEAKGNEIPQTIAPSTYVSTYLKRTPHKSQA
DTTAEKTADNVSSSTPLCVITNRPLPSTANGVTATPVLSITGTDSSVEAR
EKRRSYLTPVRDEEAESLRKARSRQARQTRRSTQGVTLTDLQEAERTFSR
SRAERQAQEQPREKPTDTEGLEGSPEKHEPSAVPATEAGEGQQPWGRSLD
EEPICHRLRCPAQPDKPTTPASPSTSRPSLYTSSHLLWTNRFSVPDSESS
ETTTNTTTAKEMDKNENEEADLDEQSSKRLSIRERRRPKERRRGTGINFW
TKDEDETDGSEEVKETWHERLSRLESGGSNPTTSDSYGDRASARARREAR
EARLATLTSRVEEDSNRDYKKLYESALTENQKLKTKLQEAQLELADIKSK
LEKVAQKQEKTSDRSSVLEMEKRERRALERKMSEMEEEMKVLTELKSDNQ
RLKDENGALIRVISKLSK*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU30285
Unit Source
Protein Phosphorylation and Ubiquitylation Unit
Location
PPU
MTA
Dundee
Notes
I182/V (like in MYPT1)
AND Q919 missing from our variant
Price
£125.00
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