cDNA Clone BTBD12

• Synonyms: SLX4
• Plasmid: pGEX6P-2-BTBD12
• Parent Plasmid: pGEX6P1
• Expressed: GST-BTBD12
• Genbank: NM_032444.2 GI:63252862*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAAACTGAGTGTGAATGAGGCTCAGCTAGGCTTCTACTTGGG
TTCACTTTCTCATCTGTCTGCCTGTCCTGGGATTGACCCTCGCTCCTCTG
AAGACCAGCCTGAAAGCCTTAAAACTGGTCAGATGATGGATGAGTCTGAT
GAGGACTTTAAAGAACTCTGCGCTAGCTTTTTCCAAAGGGTGAAAAAACA
TGGAATCAAGGAAGTGTCAGGAGAAAGGAAGACACAAAAGGCTGCCTCAA
ACGGCACTCAGATAAGAAGCAAATTGAAAAGGACCAAACAAACTGCTACC
AAGACCAAAACCCTTCAAGGCCCTGCAGAGAAGAAACCTCCGTCTGGCAG
CCAGGCCCCTAGGACTAAAAAGCAAAGGGTAACCAAATGGCAAGCAAGTG
AACCGGCCCACTCTGTGAATGGGGAGGGGGGTGTGCTTGCCTCTGCTCCA
GATCCACCTGTGCTCCGGGAAACAGCACAAAACACCCAGACGGGTAACCA
GCAAGAACCATCGCCAAACCTTTCCAGAGAGAAAACCAGAGAGAATGTGC
CCAACAGCGACTCCCAGCCTCCTCCTTCCTGTTTGACAACAGCAGTGCCA
AGTCCCTCCAAACCCCGCACAGCACAATTGGTCCTACAGCGAATGCAGCA
GTTCAAGAGAGCAGACCCCGAGCGTTTGAGACACGCTTCAGAAGAGTGCT
CCCTCGAGGCTGCGCGGGAAGAAAATGTCCCAAAGGACCCTCAAGAGGAG
ATGATGGCGGGGAATGTGTATGGGCTTGGGCCCCCTGCCCCAGAGAGCGA
CGCTGCGGTGGCCTTGACCCTGCAGCAGGAGTTTGCACGGGTAGGAGCAT
CGGCACATGATGATAGCCTGGAGGAAAAGGGTTTGTTCTTCTGCCAGATT
TGTCAAAAGAACCTCTCAGCCATGAACGTGACCCGAAGGGAACAGCATGT
GAACAGGTGCTTGGATGAAGCTGAAAAGACACTAAGACCTTCTGTGCCTC
AGATCCCTGAGTGCCCGATTTGTGGGAAACCGTTTCTTACCTTAAAGAGC
AGAACCAGTCACTTGAAGCAGTGTGCTGTGAAGATGGAGGTTGGCCCCCA
GCTCCTGCTTCAGGCTGTGCGGCTGCAGACAGCACAGCCTGAGGGTAGCA
GCAGCCCACCCATGTTCAGCTTCAGTGATCACAGTAGAGGTCTGAAACGG
AGAGGACCCACCAGCAAGAAGGAGCCACGGAAGAGGCGGAAGGTGGACGA
GGCACCGTCCGAGGACCTGCTGGTGGCCATGGCTCTGTCCCGGTCGGAGA
TGGAGCCGGGTGCGGCTGTACCAGCGCTCAGGCTGGAAAGTGCCTTTTCT
GAGAGGATAAGACCAGAAGCAGAGAATAAAAGTCGCAAGAAGAAACCCCC
GGTATCCCCCCCATTGTTGTTAGTCCAGGACTCTGAAACCACAGGCCGAC
AGATAGAGGACCGTGTGGCCCTGCTCCTCTCTGAGGAAGTGGAATTGTCT
AGCACGCCACCACTTCCTGCCAGCAGGATTTTAAAGGAAGGGTGGGAAAG
AGCGGGCCAGTGTCCTCCTCCACCTGAACGCAAGCAGAGCTTTCTGTGGG
AGGGCAGCGCACTGACTGGGGCCTGGGCCATGGAGGACTTCTACACGGCC
AGGCTGGTCCCTCCTCTCGTGCCCCAGCGGCCTGCCCAGGGCCTTATGCA
GGAGCCCGTGCCGCCTCTGGTGCCACCTGAGCACTCAGAGCTGAGCGAGC
GAAGGTCACCCGCTCTCCACGGCACCCCCACTGCAGGCTGTGGCTCCAGG
GGCCCGTCGCCTTCGGCCAGCCAGAGGGAGCACCAGGCCCTGCAGGACCT
CGTGGACCTGGCGAGGGAGGGACTGAGCGCCAGCCCGTGGCCCGGCAGTG
GGGGCCTGGCTGGCTCGGAAGGGACTGCAGGGTTGGACGTGGTGCCCGGC
GGCCTTCCTCTGACTGGGTTTGTGGTGCCATCGCAGGACAAGCACCCGGA
CAGGGGCGGCCGAACCTTGCTCTCCCTCGGGCTGCTGGTTGCTGACTTTG
GCGCCATGGTCAATAACCCACACCTGAGTGATGTCCAGTTTCAGACGGAC
AGCGGGGAGGTGCTTTACGCCCACAAGTTCGTGCTTTATGCCCGATGCCC
GCTCCTCATCCAGTATGTGAACAATGAAGGCTTCTCCGCTGTAGAGGACG
GGGTTCTGACCCAGCGTGTCCTGCTGGGTGACGTGAGCACCGAGGCCGCC
CGCACGTTCCTGCACTATCTCTACACTGCGGACACTGGCCTTCCTCCTGG
CCTTAGCTCTGAGCTGAGCTCCCTGGCCCACAGGTTTGGCGTGAGTGAGC
TCGTTCACCTGTGCGAACAGGTGCCTATTGCCACTGACTCAGAGGGCAAA
CCATGGGAGGAGAAGGAAGCAGAGAATTGCGAAAGCAGGGCCGAGAATTT
CCAGGAACTCTTGAGGTCAATGTGGGCAGATGAAGAGGAGGAAGCGGAGA
CTTTGTTGAAATCCAAGGACCACGAAGAAGATCAAGAAAACGTGAATGAA
GCAGAAATGGAAGAAATTTATGAATTTGCAGCTACTCAGCGAAAGCTTCT
CCAGGAAGAAAGGGCAGCGGGTGCCGGCGAGGACGCTGACTGGCTGGAGG
GTGGCAGTCCGGTTTCTGGGCAACTCCTAGCAGGTGTCCAGGTGCAGAAA
CAGTGGGACAAGGTGGAGGAGATGGAGCCGTTGGAGCCAGGAAGAGATGA
GGCCGCCACCACCTGGGAGAAGATGGGACAGTGCGCTCTCCCGCCACCCC
AGGGCCAGCACTCAGGGGCACGGGGAGCAGAGGCCCCTGAGCAGGAGGCG
CCAGAGGAGGCGCTTGGCCATTCCAGCTGCTCCAGCCCTTCCAGGGACTG
CCAGGCAGAGAGAAAAGAAGGCTCTCTTCCGCACTCAGATGATGCCGGGG
ATTACGAACAGCTCTTCTCATCAACTCAGGGAGAGATCTCAGAGCCGTCC
CAAATAACAAGTGAGCCCGAGGAACAAAGTGGCGCTGTCAGGGAAAGGGG
GCTGGAGGTTTCTCATCGCCTGGCTCCCTGGCAGGCATCTCCACCGCACC
CGTGCCGCTTCCTATTGGGGCCTCCCCAGGGCGGGAGTCCCCGCGGGTCT
CATCACACAAGTGGGTCGTCCCTGTCAACACCCCGGTCCCGTGGCGGAAC
TTCCCAGGTGGGCTCCCCAACCTTGCTGTCTCCAGCTGTGCCATCAAAGC
AGAAAAGGGACAGGAGCATCCTCACGCTGTCTAAAGAGCCAGGGCACCAG
AAAGGCAAAGAGCGTCGGTCCGTGCTGGAGTGCAGAAATAAGGGGGTCCT
GATGTTCCCAGAAAAATCTCTGTCTATTGACCTAACCCAGTCAAATCCTG
ACCATTCGAGCTCCAGATCTCAGAAATCTTCATCCAAACTGAACGAAGAA
GATGAGGTCATCCTCTTACTGGACTCGGATGAGGAGCTGGAGCTAGAACA
AACCAAAATGAAGTCCATTTCTAGTGATCCTCTGGAAGAAAAGAAAGCTC
TAGAAATTAGCCCTAGGTCCTGTGAGCTGTTTTCCATCATTGATGTTGAT
GCAGATCAGGAACCTTCCCAGAGCCCACCAAGAAGCGAAGCTGTGCTGCA
GCAGGAGGATGAGGGGGCGCTGCCGGAGAATCGGGGCTCTTTGGGCAGGA
GAGGGGCTCCCTGGCTGTTCTGTGACCGTGAGAGCAGCCCCAGCGAGGCC
AGCACCACAGACACCTCGTGGCTGGTGCCCGCCACCCCGCTGGCCAGCAG
AAGCCGTGACTGTTCTTCCCAGACCCAAATCAGCAGCCTCAGGAGCGGGC
TGGCCGTGCAGGCGGTGACTCAGCACACGCCCAGGGCCTCAGTAGGAAAC
AGGGAAGGGAACGAAGTCGCACAGAAGTTTTCTGTCATCAGGCCCCAGAC
ACCACCGCCCCAGACACCGTCCTCATGCCTCACTCCCGTCTCTCCAGGAA
CTTCTGACGGCAGAAGGCAAGGCCACAGAAGCCCTTCCCGTCCCCACCCC
GGGGGCCACCCGCACTCCTCTCCGCTGGCTCCACATCCCATCTCAGGGGA
CCGCGCCCACTTCAGCAGGCGGTTCCTGAAACACTCGCCGCCTGGGCCAA
GCTTCCTGAACCAGACCCCAGCGGGTGAAGTGGTGGAAGTCGGAGACAGT
GACGATGAGCAGGAGGTGGCCTCCCATCAGGCCAACAGAAGCCCCCCACT
GGACAGTGACCCCCCAATTCCAATTGACGACTGCTGCTGGCACATGGAGC
CCATCTCGCCAATTCCCATTGACCACTGGAACCTGGAGCGGACCGGCCCC
CTGAGCACCAGCAGCCCCAGCCGCAGGATGAACGAGGCCGCCGACAGCCG
TGACTGTCGCTCCCCGGGACTCCTGGACACCACCCCCATCCGAGGAAGCT
GCACTACCCAGAGGAAATTGCAAGAGAAGTCCTCGGGCGCGGGCTCCCTG
GGGAACAGCAGGCCGAGCTTTCTGAATTCGGCTCTGTGGGACGTTTGGGA
CGGGGAAGAGCAGAGGCCTCCAGAGACCCCTCCTCCGGCCCAGATGCCAA
GCGCTGGTGGAGCTCAGAAGCCCGAAGGGTTAGAGACACCCAAAGGTGCT
AATCGGAAGAAGAACTTGCCCCCCAAAGTGCCCATAACGCCGATGCCACA
GTATTCCATTATGGAGACGCCGGTGCTGAAGAAGGAACTGGATAGGTTTG
GAGTCCGCCCTCTGCCTAAACGCCAGATGGTTCTGAAGCTGAAGGAGATA
TTCCAGTACACTCACCAGACCCTGGACTCAGACTCCGAGGACGAGAGCCA
GTCCTCACAGCCGCTGTTGCAGGCGCCTCACTGCCAGACCCTCGCCTCCC
AGACCTACAAGCCTTCAAGGGCAGGGGTCCATGCCCAGCAGGAGGCCACC
ACAGGACCTGGGGCCCATAGGCCCAAGGGACCTGCTAAGACCAAGGGCCC
CCGACATCAAAGGAAGCATCATGAAAGCATCACACCCCCAAGCAGGTCGC
CCACCAAGGAGGCACCTCCAGGCCTCAATGATGACGCCCAGATCCCAGCC
TCTCAAGAATCCGTGGCCACCTCTGTGGATGGCAGTGACAGCTCCTTGAG
CTCACAGAGTTCTTCCTCCTGTGAGTTTGGAGCGGCATTTGAGTCTGCAG
GTGAAGAGGAGGGCGAGGGGGAGGTCAGTGCCTCGCAGGCAGCCGTGCAG
GCGGCGGACACAGACGAGGCGCTGAGGTGCTACATCCGCTCCAAGCCGGC
CCTGTACCAGAAGGTGCTGCTGTACCAGCCCTTTGAGCTGCGGGAGCTGC
AGGCAGAGCTGAGGCAGAACGGCCTCCGTGTGTCCTCGCGCAGGCTGTTG
GACTTCCTGGACACCCACTGTATCACCTTCACCACTGCCGCCACCCGCAG
GGAGAAGCTCCAGGGCAGGAGGCGGCAGCCTCGGGGCAAGAAGAAGGTGG
AGCGGAACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKLSVNEAQLGFYLGSLSH
LSACPGIDPRSSEDQPESLKTGQMMDESDEDFKELCASFFQRVKKHGIKE
VSGERKTQKAASNGTQIRSKLKRTKQTATKTKTLQGPAEKKPPSGSQAPR
TKKQRVTKWQASEPAHSVNGEGGVLASAPDPPVLRETAQNTQTGNQQEPS
PNLSREKTRENVPNSDSQPPPSCLTTAVPSPSKPRTAQLVLQRMQQFKRA
DPERLRHASEECSLEAAREENVPKDPQEEMMAGNVYGLGPPAPESDAAVA
LTLQQEFARVGASAHDDSLEEKGLFFCQICQKNLSAMNVTRREQHVNRCL
DEAEKTLRPSVPQIPECPICGKPFLTLKSRTSHLKQCAVKMEVGPQLLLQ
AVRLQTAQPEGSSSPPMFSFSDHSRGLKRRGPTSKKEPRKRRKVDEAPSE
DLLVAMALSRSEMEPGAAVPALRLESAFSERIRPEAENKSRKKKPPVSPP
LLLVQDSETTGRQIEDRVALLLSEEVELSSTPPLPASRILKEGWERAGQC
PPPPERKQSFLWEGSALTGAWAMEDFYTARLVPPLVPQRPAQGLMQEPVP
PLVPPEHSELSERRSPALHGTPTAGCGSRGPSPSASQREHQALQDLVDLA
REGLSASPWPGSGGLAGSEGTAGLDVVPGGLPLTGFVVPSQDKHPDRGGR
TLLSLGLLVADFGAMVNNPHLSDVQFQTDSGEVLYAHKFVLYARCPLLIQ
YVNNEGFSAVEDGVLTQRVLLGDVSTEAARTFLHYLYTADTGLPPGLSSE
LSSLAHRFGVSELVHLCEQVPIATDSEGKPWEEKEAENCESRAENFQELL
RSMWADEEEEAETLLKSKDHEEDQENVNEAEMEEIYEFAATQRKLLQEER
AAGAGEDADWLEGGSPVSGQLLAGVQVQKQWDKVEEMEPLEPGRDEAATT
WEKMGQCALPPPQGQHSGARGAEAPEQEAPEEALGHSSCSSPSRDCQAER
KEGSLPHSDDAGDYEQLFSSTQGEISEPSQITSEPEEQSGAVRERGLEVS
HRLAPWQASPPHPCRFLLGPPQGGSPRGSHHTSGSSLSTPRSRGGTSQVG
SPTLLSPAVPSKQKRDRSILTLSKEPGHQKGKERRSVLECRNKGVLMFPE
KSLSIDLTQSNPDHSSSRSQKSSSKLNEEDEVILLLDSDEELELEQTKMK
SISSDPLEEKKALEISPRSCELFSIIDVDADQEPSQSPPRSEAVLQQEDE
GALPENRGSLGRRGAPWLFCDRESSPSEASTTDTSWLVPATPLASRSRDC
SSQTQISSLRSGLAVQAVTQHTPRASVGNREGNEVAQKFSVIRPQTPPPQ
TPSSCLTPVSPGTSDGRRQGHRSPSRPHPGGHPHSSPLAPHPISGDRAHF
SRRFLKHSPPGPSFLNQTPAGEVVEVGDSDDEQEVASHQANRSPPLDSDP
PIPIDDCCWHMEPISPIPIDHWNLERTGPLSTSSPSRRMNEAADSRDCRS
PGLLDTTPIRGSCTTQRKLQEKSSGAGSLGNSRPSFLNSALWDVWDGEEQ
RPPETPPPAQMPSAGGAQKPEGLETPKGANRKKNLPPKVPITPMPQYSIM
ETPVLKKELDRFGVRPLPKRQMVLKLKEIFQYTHQTLDSDSEDESQSSQP
LLQAPHCQTLASQTYKPSRAGVHAQQEATTGPGAHRPKGPAKTKGPRHQR
KHHESITPPSRSPTKEAPPGLNDDAQIPASQESVATSVDGSDSSLSSQSS
SSCEFGAAFESAGEEEGEGEVSASQAAVQAADTDEALRCYIRSKPALYQK
VLLYQPFELRELQAELRQNGLRVSSRRLLDFLDTHCITFTTAATRREKLQ
GRRRQPRGKKKVERN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16046
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_032444.2 protein sequence:; P1122L ; L1433I; Bacterial construct to express GST N terminal fusion.; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.; Estimated MW of expressed protein : 226843 Daltons