cDNA Clone AS160

• Synonyms: TBC1D4
• Plasmid: pCMV5-HA AS160(B) P281-I770 (I762 in AS160(B))
• Parent Plasmid: pCMV5 HA1
• Expressed: HA AS160(B) P281-I770
• Genbank: NM_014832*
• Tag: HA
• Species: Human

Sequence of Insert

ggatccCCTGCCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTG
CTTCCCTGAGCGGATTTTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGT
TTCGGTCTCGGTGCAGCAGTGTCACCGGCGTGCAACGGAGAGTTCACGAG
GGCAGCCAGAAATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCA
CGTCCAGCCCTCGGACTCGGAGAAGAACAGGACCATGCTCTTCCAGGTTG
GGCGATTTGAGATTAACCTTATCAGTCCAGACACTAAATCAGTTGTGCTA
GAAAAGAATTTTAAAGATATCTCCCCTTGTTCTCAGGGTATAAAGCATGT
GGATCACTTTGGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCC
AGTATATTTGTTATGTATTCCAGTGTGCCAGCGAATCTCTGGTTGATGAG
GTAATGCTGACTCTGAAACAGGCCTTCAGTACGGCGGCTGCCCTGCAGAG
TGCCAAGACGCAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCTTTGC
ATAAGCTCTGTGAAAGGATTGAAGGTCTCTACCCACCAAGAGCCAAGCTG
GTGATACAGAGGCATCTCTCATCACTGACAGATAATGAGCAAGCTGACAT
CTTTGAAAGAGTTCAGAAAATGAAGCCAGTCAGTGACCAGGAAGAAAATG
AACTTGTGATTTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACA
CACGTGCACATCGGGGAAGGCCCTGCTACTATTTCAAATAGTACAATCCC
AGAAAATGCAACAAGCAGTGGAAGGTTCAAACTTGACATTCTGAAAAATA
AAGCTAAGAGATCCTTAACTAGCTCCCTGGAAAATATCTTCTCAAGGGGA
GCTAACAGAATGAGAGGTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTC
CAACAGTCTTGCTTCAGAGAAGGACTACTCACCAGGGGATTCTCCACCAG
GGACACCGCCAGCGTCCCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAA
GAGGATTCCGACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGCCA
CCCACCTTCAAGCACAAAGAGAAAGCTGAATTTGCAGGATGGGAGGGCTC
AGGGTGTGCGTTCCCCTCTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGC
AATCTTTCGTCAGTTCGACGCATGTACAAGGAGAGTAATTCTTCCTCCAG
TCTTCCAAGTCTTCACACTTCCTTCTCTGCCCCTTCCTTCACTGCCCCCT
CTTTCCTGAAAAGCTTTTACCAGAATTCAGGTAGACTGTCCCCACAGTAT
GAAAATGAAATCAGTGATGGAGAAGGGAGAAAAAGGACCTCATCTACCTG
CAGCAATGAGTCCCTAAGTGTGGGAGGAACCTCTGTCACTCCTCGCCGGA
TCtaagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSS
VTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINL
ISPDTKSVVLEKNFKDISPCSQGIKHVDHFGFICRESPEPGLSQYICYVF
QCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERI
EGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHL
RQLCEAKQKTHVHIGEGPATISNSTIPENATSSGRFKLDILKNKAKRSLT
SSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASP
PSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPL
LRQSSSEQCSNLSSVRRMYKESNSSSSLPSLHTSFSAPSFTAPSFLKSFY
QNSGRLSPQYENEISDGEGRKRTSSTCSNESLSVGGTSVTPRRI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16018
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences in cloned AS160 (B) (long form) compared with NM_014832: ; S387P, S537A, K958R, delta R733-S740, delta G865. These differences are present in all AS160 (long form) DU clones.; ; EStimated MW of expressed protein : 54838 Daltons