cDNA Clone DISC1

• Plasmid: pEBG6P-1 DISC1
• Parent Plasmid: pEBG6P
• Expressed: GST-DISC1
• Genbank: AF222980.1 GI:8163868
• Tag: GST
• Species: Human

Sequence of Insert

ACTAGTATGCCAGGCGGGGGTCCTCAGGGCGCCCCAGCCGCCGCCGGCGGCGGCGGCGTGAGCCACCGCGCAGGCAGCCGGGATTGCTTACCACCTGCAGCGTGCTTTCGGAGGCGGCGGCTGGCACGGAGGCCGGGCTACATGAGAAGCTCGACAGGGCCTGGGATCGGGTTCCTTTCCCCAGCAGTGGGCACACTGTTCCGGTTCCCAGGAGGGGTGTCTGGCGAGGAGTCCCACCACTCGGAGTCCAGGGCCAGACAGTGTGGCCTTGACTCGAGAGGCCTCTTGGTCCGGAGCCCTGTTTCC
AAGAGTGCAGCAGCCCCTACTGTGACCTCTGTGAGAGGAACCTCGGCGCA
CTTTGGGATTCAGCTCAGAGGTGGCACCAGATTGCCTGACAGGCTTAGCT
GGCCGTGTGGCCCTGGGAGTGCTGGGTGGCAGCAAGAGTTTGCAGCCATG
GATAGTTCTGAGACCCTGGACGCCAGCTGGGAGGCAGCCTGCAGCGATGG
AGCAAGGCGTGTCCGGGCAGCAGGCTCTCTGCCATCAGCAGAGTTGAGTA
GCAACAGCTGCAGCCCTGGCTGTGGCCCTGAGGTCCCCCCAACCCCTCCT
GGCTCTCACAGTGCCTTTACCTCAAGCTTTAGCTTTATTCGGCTCTCGCT
TGGCTCTGCCGGGGAACGTGGAGAAGCAGAAGGCTGCCCACCATCCAGAG
AGGCTGAGTCCCATTGCCAGAGCCCCCAGGAGATGGGAGCCAAAGCTGCC
AGCTTGGACGGGCCTCACGAGGACCCGCGATGTCTCTCTCAGCCCTTCAG
TCTCTTGGCTACACGGGTCTCTGCAGACTTGGCCCAGGCCGCAAGGAACA
GCTCCAGGCCAGAGCGTGACATGCATTCTTTACCAGACATGGACCCTGGC
TCCTCCAGTTCTCTGGATCCCTCACTGGCTGGCTGTGGTGGTGATGGGAG
CAGCGGCTCAGGGGATGCCCACTCTTGGGACACCCTGCTCAGGAAATGGG
AGCCAGTGCTGCGGGACTGCCTGCTGAGAAACCGGAGGCAGATGGAGGTA
ATATCCTTAAGATTAAAACTTCAGAAACTTCAGGAAGATGCAGTTGAGAA
TGATGATTATGATAAAGCTGAGACGTTACAACAAAGATTAGAAGACCTGG
AACAAGAGAAAATCAGCCTGCACTTTCAACTTCCTTCAAGGCAGCCAGCT
CTTAGCAGTTTCCTGGGTCACCTGGCAGCACAAGTCCAGGCTGCCTTGCG
CCGTGGGGCCACTCAGCAGGCCAGCGGAGATGACACCCACACCCCACTGA
GAATGGAGCCGAGGCTGTTGGAACCCACTGCTCAGGACAGCTTGCACGTG
TCCATCACGAGACGAGACTGGCTTCTTCAGGAAAAGCAGCAGCTACAGAA
AGAAATCGAAGCTCTCCAAGCAAGGATGTTTGTGCTGGAAGCCAAAGATC
AACAGCTGAGAAGGGAAATAGAGGAGCAAGAGCAGCAACTCCAGTGGCAG
GGCTGCGACCTGACCCCACTGGTGGGCCAGCTGTCCCTGGGTCAGCTGCA
GGAGGTCAGCAAGGCCTTGCAGGACACCCTGGCCTCAGCCGGTCAGATTC
CCTTCCATGCAGAGCCACCGGAAACCATAAGGAGCCTCCAGGAAAGAATA
AAATCCCTCAACTTGTCACTTAAAGAAATCACTACTAAGGTGTGTATGAG
TGAGAAATTCTGCAGCACCCTGAGGAAGAAAGTTAACGATATTGAAACCC
AACTACCAGCCTTGCTTGAAGCCAAAATGCATGCCATATCAGGAAACCAT
TTCTGGACGGCTAAAGACCTCACCGAGGAGATTAGATCATTAACATCAGA
GAGAGAAGGGCTGGAGGGACTCCTCAGCAAGCTGTTGGTGTTGAGTTCCA
GGAATGTCAAAAAGCTGGGAAGTGTTAAAGAAGATTACAACAGACTGAGA
AGAGAAGTGGAGCACCAGGAGACTGCCTATGAAACAAGTGTGAAGGAAAA
TACTATGAAGTACATGGAAACACTTAAGAATAAACTGTGCAGCTGCAAGT
GTCCACTGCTTGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGATTG
CTTATCCAGTGCCTACAGCTCCAGGAAGCCAGGGGAAGCCTGTCTGTAGA
AGATGAGAGGCAGATGGATGACTTAGAGGGAGCTGCTCCTCCTATTCCCC
CCAGGCTCCACTCCGAGGATAAAAGGAAGACCCCTTTGAAGGTATTGGAA
GAATGGAAGACTCACCTCATCCCCTCTCTGCACTGTGCTGGAGGTGAACA
GAAAGAGGAATCTTACATCCTTTCTGCAGAACTTGGAGAAAAGTGTGAAG
ACATAGGCAAGAAGCTATTGTACTTGGAAGATCAACTTCACACAGCAATC
CACAGTCATGATGAAGATCTCATTCAGTCTCTCAGGAGGGAGCTCCAGAT
GGTGAAGGAAACTCTGCAGGCCATGATCCTGCAGCTCCAGCCAGCAAAGG
AGGCGGGAGAAAGAGAAGCTGCAGCTTCCTGCATGACAGCTGGTGTCCAC
GAAGCACAAGCCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMPGGGPQGAPA
AAGGGGVSHRAGSRDCLPPAACFRRRRLARRPGYMRSSTGPGIGFLSPAV
GTLFRFPGGVSGEESHHSESRARQCGLDSRGLLVRSPVSKSAAAPTVTSV
RGTSAHFGIQLRGGTRLPDRLSWPCGPGSAGWQQEFAAMDSSETLDASWE
AACSDGARRVRAAGSLPSAELSSNSCSPGCGPEVPPTPPGSHSAFTSSFS
FIRLSLGSAGERGEAEGCPPSREAESHCQSPQEMGAKAASLDGPHEDPRC
LSQPFSLLATRVSADLAQAARNSSRPERDMHSLPDMDPGSSSSLDPSLAG
CGGDGSSGSGDAHSWDTLLRKWEPVLRDCLLRNRRQMEVISLRLKLQKLQ
EDAVENDDYDKAETLQQRLEDLEQEKISLHFQLPSRQPALSSFLGHLAAQ
VQAALRRGATQQASGDDTHTPLRMEPRLLEPTAQDSLHVSITRRDWLLQE
KQQLQKEIEALQARMFVLEAKDQQLRREIEEQEQQLQWQGCDLTPLVGQL
SLGQLQEVSKALQDTLASAGQIPFHAEPPETIRSLQERIKSLNLSLKEIT
TKVCMSEKFCSTLRKKVNDIETQLPALLEAKMHAISGNHFWTAKDLTEEI
RSLTSEREGLEGLLSKLLVLSSRNVKKLGSVKEDYNRLRREVEHQETAYE
TSVKENTMKYMETLKNKLCSCKCPLLGKVWEADLEACRLLIQCLQLQEAR
GSLSVEDERQMDDLEGAAPPIPPRLHSEDKRKTPLKVLEEWKTHLIPSLH
CAGGEQKEESYILSAELGEKCEDIGKKLLYLEDQLHTAIHSHDEDLIQSL
RRELQMVKETLQAMILQLQPAKEAGEREAAASCMTAGVHEAQA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11985
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express DISC1 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 121199 Daltons