cDNA Clone ATR

• Plasmid: pFBDM FLAG-ATRIP/ATR
• Parent Plasmid: pFBDM
• Expressed: ATR
• Genbank: NP_001175.2 GI:157266317
• Tag: untagged
• Species: Human

Sequence of Insert

gcggccgcatgggggaacatggcctggagctggcttccatgatccccgccctgcgggagctgggcagtgccacaccagaggaatataatacagttgtacagaagccaagacaaattctgtgtcaattcattgaccggatacttacagatgtaaatgttgttgctgtagaacttgtaaagaaaactgactctcagccaacctccgtgatgttgcttgatttcatccagcatatcatgaaatcctccccacttatgtttgtaaatgtgagtggaagccatgaggccaaaggcagttgtattgaattcagtaattggatcataacgagacttctgcggattgcagcaactccctcctgtcatttgttacacaagaaaatctgtgaagtcatctgttcattattatttcttt ttaaaagcaagagtcctgctatttttggggtactcacaaaagaattatta caactttttgaagacttggtttacctccatagaagaaatgtgatgggtca tgctgtggaatggccagtggtcatgagccgatttttaagtcaattagatg aacacatgggatatttacaatcagctcctttgcagttgatgagtatgcaa aatttagaatttattgaagtcactttattaatggttcttactcgtattat tgcaattgtgttttttagaaggcaagaactcttactttggcagataggtt gtgttctgctagagtatggtagtccaaaaattaaatccctagcaattagc tttttaacagaactttttcagcttggaggactaccagcacaaccagctag cacttttttcagctcatttttggaattattaaaacaccttgtagaaatgg atactgaccaattgaaactctatgaagagccattatcaaagctgataaag acactatttccctttgaagcagaagcttatagaaatattgaacctgtcta tttaaatatgctgctggaaaaactctgtgtcatgtttgaagacggtgtgc tcatgcggcttaagtctgatttgctaaaagcagctttgtgccatttactg cagtatttccttaaatttgtgccagctgggtatgaatctgctttacaagt caggaaggtctatgtgagaaatatttgtaaagctcttttggatgtgcttg gaattgaggtagatgcagagtacttgttgggcccactttatgcagctttg aaaatggaaagtatggaaatcattgaggagattcaatgccaaactcaaca ggaaaacctcagcagtaatagtgatggaatatcacccaaaaggcgtcgtc tcagctcgtctctaaacccttctaaaagagcaccaaaacagactgaggaa attaaacatgtggacatgaaccaaaagagcatattatggagtgcactgaa acagaaagctgaatcccttcagatttcccttgaatacagtggcctaaaga atcctgttattgagatgttagaaggaattgctgttgtcttacaactgact gctctgtgtactgttcattgttctcatcaaaacatgaactgccgtacttt caaggactgtcaacataaatccaagaagaaaccttctgtagtgataactt ggatgtcattggatttttacacaaaagtgcttaagagctgtagaagtttg ttagaatctgttcagaaactggacctggaggcaaccattgataaggtggt gaaaatttatgatgctttgatttatatgcaagtaaacagttcatttgaag atcatatcctggaagatttatgtggtatgctctcacttccatggatttat tcccattctgatgatggctgtttaaagttgaccacatttgccgctaatct tctaacattaagctgtaggatttcagatagctattcaccacaggcacaat cacgatgtgtgtttcttctgactctgtttccaagaagaatattccttgag tggagaacagcagtttacaactgggccctgcagagctcccatgaagtaat ccgggctagttgtgttagtggattttttatcttattgcagcagcagaatt cttgtaacagagttcccaagattcttatagataaagtcaaagatgattct gacattgtcaagaaagaatttgcttctatacttggtcaacttgtctgtac tcttcacggcatgttttatctgacaagttctttaacagaacctttctctg aacacggacatgtggacctcttctgtaggaacttgaaagccacttctcaa catgaatgttcatcttctcaactaaaagcttctgtctgcaagccattcct tttcctactgaaaaaaaaaatacctagtccagtaaaacttgctttcatag ataatctacatcatctttgtaagcatcttgattttagagaagatgaaaca gatgtaaaagcagttcttggaactttattaaatttaatggaagatccaga caaagatgttagagtggcttttagtggaaatatcaagcacatattggaat ccttggactctgaagatggatttataaaggagctttttgtcttaagaatg aaggaagcatatacacatgcccaaatatcaagaaataatgagctgaagga taccttgattcttacaacaggggatattggaagggccgcaaaaggagatt tggtaccatttgcactcttacacttattgcattgtttgttatccaagtca gcatctgtctctggagcagcatacacagaaattagagctctggttgcagc taaaagtgttaaactgcaaagttttttcagccagtataagaaacccatct gtcagtttttggtagaatcccttcactctagtcagatgacagcacttccg aatactccatgccagaatgctgacgtgcgaaaacaagatgtggctcacca gagagaaatggctttaaatacgttgtctgaaattgccaacgttttcgact ttcctgatcttaatcgttttcttactaggacattacaagttctactacct gatcttgctgccaaagcaagccctgcagcttctgctctcattcgaacttt aggaaaacaattaaatgtcaatcgtagagagattttaataaacaacttca aatatattttttctcatttggtctgttcttgttccaaagatgaattagaa cgtgcccttcattatctgaagaatgaaacagaaattgaactggggagcct gttgagacaagatttccaaggattgcataatgaattattgctgcgtattg gagaacactatcaacaggtttttaatggtttgtcaatacttgcctcattt gcatccagtgatgatccatatcagggcccgagagatatcatatcacctga actgatggctgattatttacaacccaaattgttgggcattttggcttttt ttaacatgcagttactgagctctagtgttggcattgaagataagaaaatg gccttgaacagtttgatgtctttgatgaagttaatgggacccaaacatgt cagttctgtgagggtgaagatgatgaccacactgagaactggccttcgat tcaaggatgattttcctgaattgtgttgcagagcttgggactgctttgtt cgctgcctggatcatgcttgtctgggctcccttctcagtcatgtaatagt agctttgttacctcttatacacatccagcctaaagaaactgcagctatct tccactacctcataattgaaaacagggatgctgtgcaagattttcttcat gaaatatattttttacctgatcatccagaattaaaaaagataaaagccgt tctccaggaatacagaaaggagacctctgagagcactgatcttcagacaa ctcttcagctctctatgaaggccattcaacatgaaaatgtcgatgttcgt attcatgctcttacaagcttgaaggaaaccttgtataaaaatcaggaaaa actgataaagtatgcaacagacagtgaaacagtagaacctattatctcac agttggtgacagtgcttttgaaaggttgccaagatgcaaactctcaagct cggttgctctgtggggaatgtttaggggaattgggggcgatagatccagg tcgattagatttctcaacaactgaaactcaaggaaaagattttacatttg tgactggagtagaagattcaagctttgcctatggattattgatggagcta acaagagcttaccttgcgtacgctgataatagccgagctcaagattcagc tgcctatgccattcaggagttgctttctatttatgactgtagagagatgg agaccaacggcccaggtcaccaattgtggaggagatttcctgagcatgtt cgggaaatactagaacctcatctaaataccagatacaagagttctcagaa gtcaaccgattggtctggagtaaagaagccaatttacttaagtaaattgg gtagtaactttgcagaatggtcagcatcttgggcaggttatcttattaca aaggttcgacatgatcttgccagtaaaattttcacctgctgtagcattat gatgaagcatgatttcaaagtgaccatctatcttcttccacatattctgg tgtatgtcttactgggttgtaatcaagaagatcagcaggaggtttatgca gaaattatggcagttctaaagcatgacgatcagcataccataaataccca agacattgcatctgatctgtgtcaactcagtacacagactgtgttctcca tgcttgaccatctcacacagtgggcaaggcacaaatttcaggcactgaaa gctgagaaatgtccacacagcaaatcaaacagaaataaggtagactcaat ggtatctactgtggattatgaagactatcagagtgtaacccgttttctag acctcataccccaggatactctggcagtagcttcctttcgctccaaagca tacacacgagctgtaatgcactttgaatcatttattacagaaaagaagca aaatattcaggaacatcttggatttttacagaaattgtatgctgctatgc atgaacctgatggagtggccggagtcagtgcaattagaaaggcagaacca tctctaaaagaacagatccttgaacatgaaagccttggcttgctgaggga tgccactgcttgttatgacagggctattcagctagaaccagaccagatca ttcattatcatggtgtagtaaagtccatgttaggtcttggtcagctgtct actgttatcactcaggtgaatggagtgcatgctaacaggtccgagtggac agatgaattaaacacgtacagagtggaagcagcttggaaattgtcacagt gggatttggtggaaaactatttggcagcagatggaaaatctacaacatgg agtgtcagactgggacagctattattatcagccaaaaaaagagatatcac agctttttatgactcactgaaactagtgagagcagaacaaattgtacctc tttcagctgcaagctttgaaagaggctcctaccaacgaggatatgaatat attgtgagattgcacatgttatgtgagttggagcatagcatcaaaccact tttccagcattctccaggtgacagttctcaagaagattctctaaactggg tagctcgactagaaatgacccagaattcctacagagccaaggagcctatc ctggctctccggagggctttactaagcctcaacaaaagaccagattacaa tgaaatggttggagaatgctggctgcagagtgccagggtagctagaaagg ctggtcaccaccagacagcctacaatgctctccttaatgcaggggaatca cgactcgctgaactgtacgtggaaagggcaaagtggctctggtccaaggg tgatgttcaccaggcactaattgttcttcaaaaaggtgttgaattatgtt ttcctgaaaatgaaaccccacctgagggtaagaacatgttaatccatggt cgagctatgctactagtgggccgatttatggaagaaacagctaactttga aagcaatgcaattatgaaaaaatataaggatgtgaccgcgtgcctgccag aatgggaggatgggcatttttaccttgccaagtactatgacaaattgatg cccatggtcacagacaacaaaatggaaaagcaaggtgatctcatccggta tatagttcttcattttggcagatctctacaatatggaaatcagttcatat atcagtcaatgccacgaatgttaactctatggcttgattatggtacaaag gcatatgaatgggaaaaagctggccgctccgatcgtgtacaaatgaggaa tgatttgggtaaaataaacaaggttatcacagagcatacaaactatttag ctccatatcaatttttgactgctttttcacaattgatctctcgaatttgt cattctcacgatgaagtttttgttgtcttgatggaaataatagccaaagt atttctagcctatcctcaacaagcaatgtggatgatgacagctgtgtcaa agtcatcttatcccatgcgtgtgaacagatgcaaggaaatcctcaataaa gctattcatatgaaaaaatccttagagaagtttgttggagatgcaactcg cctaacagataagcttctagaattgtgcaataaaccggttgatggaagta gttccacattaagcatgagcactcattttaaaatgcttaaaaagctggta gaagaagcaacatttagtgaaatcctcattcctctacaatcagtcatgat acctacacttccatcaattctgggtacccatgctaaccatgctagccatg aaccatttcctggacattgggcctatattgcagggtttgatgatatggtg gaaattcttgcttctcttcagaaaccaaagaagatttctttaaaaggctc agatggaaagttctacatcatgatgtgtaagccaaaagatgacctgagaa aggattgtagactaatggaattcaattccttgattaataagtgcttaaga aaagatgcagagtctcgtagaagagaacttcatattcgaacatatgcagt tattccactaaatgatgaatgtgggattattgaatgggtgaacaacactg ctggtttgagacctattctgaccaaactatataaagaaaagggagtgtat atgacaggaaaagaacttcgccagtgtatgctaccaaagtcagcagcttt atctgaaaaactcaaagtattccgagaatttctcctgcccaggcatcctc ctatttttcatgagtggtttctgagaacattccctgatcctacatcatgg tacagtagtagatcagcttactgccgttccactgcagtaatgtcaatggt tggttatattctggggcttggagaccgtcatggtgaaaatattctctttg attctttgactggtgaatgcgtacatgtagatttcaattgtcttttcaat aagggagaaacctttgaagttccagaaattgtgccatttcgcctgactca taatatggttaatggaatgggtcctatgggaacagagggtctttttcgaa gagcatgtgaagttacaatgaggctgatgcgtgatcagcgagagccttta atgagtgtcttaaagacttttctacatgatcctcttgtggaatggagtaa accagtgaaagggcattccaaagcgccactgaatgaaactggagaagttg tcaatgaaaaggccaagacccatgttcttgacattgagcagcgactacaa ggtgtaatcaagactcgaaatagagtgacaggactgccgttatctattga aggacatgtgcattaccttatacaggaagctactgatgaaaacttactat gccagatgtatcttggttggactccatatatgtgaAATTATGTAAAAGAATATGTTAATAATCTAAAAGTAATGCATTTGGTATGAATCTGTGGTTGTATCTGTTCAATTCTAAAGTACAACATAAATTTACGTT
CTCAGCAACTGTTATTTCTCTCTGATCATTAATTATATGTAAAATAATATACATTCACTCGTGCCGAATTCGGCACGAGAATTGGCACTGATGAGGATTT
AGCGCACTCTTCTATCAGGTTTGGAATTGGCCGCTTCACTACAGAGGAGGAAGTGGACTACACAGTGGAGAAATGCATTCAGCATGTGAAGCGTCTTCGA
GAAATGAGCCCTCTCTGGGAGATGGTTCAGGATGGCATTGACCTCAAGAGCATCAAGTGGACCCAACACTAGAAGAATAGGGCCCTGACTTTGTGCTGGT
CTGGCCCTCGTGCCGCTCGTGCCGAATTCCTGCAGCCCGGGGGATCCCCTAGG
gcggccgc

Amino Acid Sequence

MGEHGLELASMIPALRELGSATPEEYNTVVQKPRQILCQFIDRILTDVNVVAVELVKKTDSQPTSVMLLDFIQHIMKSSPLMFVNVSGSHEAKGSCIEFS
NWIITRLLRIAATPSCHLLHKKICEVICSLLFLFKSKSPAIFGVLTKELLQLFEDLVYLHRRNVMGHAVEWPVVMSRFLSQLDEHMGYLQSAPLQLMSMQ
NLEFIEVTLLMVLTRIIAIVFFRRQELLLWQIGCVLLEYGSPKIKSLAISFLTELFQLGGLPAQPASTFFSSFLELLKHLVEMDTDQLKLYEEPLSKLIK
TLFPFEAEAYRNIEPVYLNMLLEKLCVMFEDGVLMRLKSDLLKAALCHLLQYFLKFVPAGYESALQVRKVYVRNICKALLDVLGIEVDAEYLLGPLYAAL
KMESMEIIEEIQCQTQQENLSSNSDGISPKRRRLSSSLNPSKRAPKQTEEIKHVDMNQKSILWSALKQKAESLQISLEYSGLKNPVIEMLEGIAVVLQLT
ALCTVHCSHQNMNCRTFKDCQHKSKKKPSVVITWMSLDFYTKVLKSCRSLLESVQKLDLEATIDKVVKIYDALIYMQVNSSFEDHILEDLCGMLSLPWIY
SHSDDGCLKLTTFAANLLTLSCRISDSYSPQAQSRCVFLLTLFPRRIFLEWRTAVYNWALQSSHEVIRASCVSGFFILLQQQNSCNRVPKILIDKVKDDS
DIVKKEFASILGQLVCTLHGMFYLTSSLTEPFSEHGHVDLFCRNLKATSQHECSSSQLKASVCKPFLFLLKKKIPSPVKLAFIDNLHHLCKHLDFREDET
DVKAVLGTLLNLMEDPDKDVRVAFSGNIKHILESLDSEDGFIKELFVLRMKEAYTHAQISRNNELKDTLILTTGDIGRAAKGDLVPFALLHLLHCLLSKS
ASVSGAAYTEIRALVAAKSVKLQSFFSQYKKPICQFLVESLHSSQMTALPNTPCQNADVRKQDVAHQREMALNTLSEIANVFDFPDLNRFLTRTLQVLLP
DLAAKASPAASALIRTLGKQLNVNRREILINNFKYIFSHLVCSCSKDELERALHYLKNETEIELGSLLRQDFQGLHNELLLRIGEHYQQVFNGLSILASF
ASSDDPYQGPRDIISPELMADYLQPKLLGILAFFNMQLLSSSVGIEDKKMALNSLMSLMKLMGPKHVSSVRVKMMTTLRTGLRFKDDFPELCCRAWDCFV
RCLDHACLGSLLSHVIVALLPLIHIQPKETAAIFHYLIIENRDAVQDFLHEIYFLPDHPELKKIKAVLQEYRKETSESTDLQTTLQLSMKAIQHENVDVR
IHALTSLKETLYKNQEKLIKYATDSETVEPIISQLVTVLLKGCQDANSQARLLCGECLGELGAIDPGRLDFSTTETQGKDFTFVTGVEDSSFAYGLLMEL
TRAYLAYADNSRAQDSAAYAIQELLSIYDCREMETNGPGHQLWRRFPEHVREILEPHLNTRYKSSQKSTDWSGVKKPIYLSKLGSNFAEWSASWAGYLIT
KVRHDLASKIFTCCSIMMKHDFKVTIYLLPHILVYVLLGCNQEDQQEVYAEIMAVLKHDDQHTINTQDIASDLCQLSTQTVFSMLDHLTQWARHKFQALK
AEKCPHSKSNRNKVDSMVSTVDYEDYQSVTRFLDLIPQDTLAVASFRSKAYTRAVMHFESFITEKKQNIQEHLGFLQKLYAAMHEPDGVAGVSAIRKAEP
SLKEQILEHESLGLLRDATACYDRAIQLEPDQIIHYHGVVKSMLGLGQLSTVITQVNGVHANRSEWTDELNTYRVEAAWKLSQWDLVENYLAADGKSTTW
SVRLGQLLLSAKKRDITAFYDSLKLVRAEQIVPLSAASFERGSYQRGYEYIVRLHMLCELEHSIKPLFQHSPGDSSQEDSLNWVARLEMTQNSYRAKEPI
LALRRALLSLNKRPDYNEMVGECWLQSARVARKAGHHQTAYNALLNAGESRLAELYVERAKWLWSKGDVHQALIVLQKGVELCFPENETPPEGKNMLIHG
RAMLLVGRFMEETANFESNAIMKKYKDVTACLPEWEDGHFYLAKYYDKLMPMVTDNKMEKQGDLIRYIVLHFGRSLQYGNQFIYQSMPRMLTLWLDYGTK
AYEWEKAGRSDRVQMRNDLGKINKVITEHTNYLAPYQFLTAFSQLISRICHSHDEVFVVLMEIIAKVFLAYPQQAMWMMTAVSKSSYPMRVNRCKEILNK
AIHMKKSLEKFVGDATRLTDKLLELCNKPVDGSSSTLSMSTHFKMLKKLVEEATFSEILIPLQSVMIPTLPSILGTHANHASHEPFPGHWAYIAGFDDMV
EILASLQKPKKISLKGSDGKFYIMMCKPKDDLRKDCRLMEFNSLINKCLRKDAESRRRELHIRTYAVIPLNDECGIIEWVNNTAGLRPILTKLYKEKGVY
MTGKELRQCMLPKSAALSEKLKVFREFLLPRHPPIFHEWFLRTFPDPTSWYSSRSAYCRSTAVMSMVGYILGLGDRHGENILFDSLTGECVHVDFNCLFN
KGETFEVPEIVPFRLTHNMVNGMGPMGTEGLFRRACEVTMRLMRDQREPLMSVLKTFLHDPLVEWSKPVKGHSKAPLNETGEVVNEKAKTHVLDIEQRLQ
GVIKTRNRVTGLPLSIEGHVHYLIQEATDENLLCQMYLGWTPYM*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU11872
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: (insert contains extra sequence (approx 460 bp) between stop codon of ATR and 3' NotI site - derives from source clone)