cDNA Clone DISC1

• Plasmid: pETBlue2.6His DISC1
• Parent Plasmid: pETBlue2 6His
• Expressed: 6His DISC1
• Genbank: AF222980.1 GI:8163868
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCATGCCAGGCGGGGGTCCTCAGGGCGCCCCAGCCGCCGCCGGCGGCGGCGGCGTGAGCCACCGCGCAGGCAGCCGGGATTGCTTACCACCTGCAGCGTGCTTTCGGAGGCGGCGGCTGGCACGGAGGCCGGGCTACATGAGAAGCTCGACAGGGCCTGGGATCGGGTTCCTTTCCCCAGCAGTGGGCACACTGTTCCGGTTCCCAGGAGGGGTGTCTGGCGAGGAGTCCCACCACTCGGAGTCCAGGGCCAGACAGTGTGGCCTTGACTCGAGAGGCCTCTTGGTCCGGAGCCCTGTTTCC
AAGAGTGCAGCAGCCCCTACTGTGACCTCTGTGAGAGGAACCTCGGCGCA
CTTTGGGATTCAGCTCAGAGGTGGCACCAGATTGCCTGACAGGCTTAGCT
GGCCGTGTGGCCCTGGGAGTGCTGGGTGGCAGCAAGAGTTTGCAGCCATG
GATAGTTCTGAGACCCTGGACGCCAGCTGGGAGGCAGCCTGCAGCGATGG
AGCAAGGCGTGTCCGGGCAGCAGGCTCTCTGCCATCAGCAGAGTTGAGTA
GCAACAGCTGCAGCCCTGGCTGTGGCCCTGAGGTCCCCCCAACCCCTCCT
GGCTCTCACAGTGCCTTTACCTCAAGCTTTAGCTTTATTCGGCTCTCGCT
TGGCTCTGCCGGGGAACGTGGAGAAGCAGAAGGCTGCCCACCATCCAGAG
AGGCTGAGTCCCATTGCCAGAGCCCCCAGGAGATGGGAGCCAAAGCTGCC
AGCTTGGACGGGCCTCACGAGGACCCGCGATGTCTCTCTCAGCCCTTCAG
TCTCTTGGCTACACGGGTCTCTGCAGACTTGGCCCAGGCCGCAAGGAACA
GCTCCAGGCCAGAGCGTGACATGCATTCTTTACCAGACATGGACCCTGGC
TCCTCCAGTTCTCTGGATCCCTCACTGGCTGGCTGTGGTGGTGATGGGAG
CAGCGGCTCAGGGGATGCCCACTCTTGGGACACCCTGCTCAGGAAATGGG
AGCCAGTGCTGCGGGACTGCCTGCTGAGAAACCGGAGGCAGATGGAGGTA
ATATCCTTAAGATTAAAACTTCAGAAACTTCAGGAAGATGCAGTTGAGAA
TGATGATTATGATAAAGCTGAGACGTTACAACAAAGATTAGAAGACCTGG
AACAAGAGAAAATCAGCCTGCACTTTCAACTTCCTTCAAGGCAGCCAGCT
CTTAGCAGTTTCCTGGGTCACCTGGCAGCACAAGTCCAGGCTGCCTTGCG
CCGTGGGGCCACTCAGCAGGCCAGCGGAGATGACACCCACACCCCACTGA
GAATGGAGCCGAGGCTGTTGGAACCCACTGCTCAGGACAGCTTGCACGTG
TCCATCACGAGACGAGACTGGCTTCTTCAGGAAAAGCAGCAGCTACAGAA
AGAAATCGAAGCTCTCCAAGCAAGGATGTTTGTGCTGGAAGCCAAAGATC
AACAGCTGAGAAGGGAAATAGAGGAGCAAGAGCAGCAACTCCAGTGGCAG
GGCTGCGACCTGACCCCACTGGTGGGCCAGCTGTCCCTGGGTCAGCTGCA
GGAGGTCAGCAAGGCCTTGCAGGACACCCTGGCCTCAGCCGGTCAGATTC
CCTTCCATGCAGAGCCACCGGAAACCATAAGGAGCCTCCAGGAAAGAATA
AAATCCCTCAACTTGTCACTTAAAGAAATCACTACTAAGGTGTGTATGAG
TGAGAAATTCTGCAGCACCCTGAGGAAGAAAGTTAACGATATTGAAACCC
AACTACCAGCCTTGCTTGAAGCCAAAATGCATGCCATATCAGGAAACCAT
TTCTGGACGGCTAAAGACCTCACCGAGGAGATTAGATCATTAACATCAGA
GAGAGAAGGGCTGGAGGGACTCCTCAGCAAGCTGTTGGTGTTGAGTTCCA
GGAATGTCAAAAAGCTGGGAAGTGTTAAAGAAGATTACAACAGACTGAGA
AGAGAAGTGGAGCACCAGGAGACTGCCTATGAAACAAGTGTGAAGGAAAA
TACTATGAAGTACATGGAAACACTTAAGAATAAACTGTGCAGCTGCAAGT
GTCCACTGCTTGGGAAAGTGTGGGAAGCTGACTTGGAAGCTTGTCGATTG
CTTATCCAGTGCCTACAGCTCCAGGAAGCCAGGGGAAGCCTGTCTGTAGA
AGATGAGAGGCAGATGGATGACTTAGAGGGAGCTGCTCCTCCTATTCCCC
CCAGGCTCCACTCCGAGGATAAAAGGAAGACCCCTTTGAAGGTATTGGAA
GAATGGAAGACTCACCTCATCCCCTCTCTGCACTGTGCTGGAGGTGAACA
GAAAGAGGAATCTTACATCCTTTCTGCAGAACTTGGAGAAAAGTGTGAAG
ACATAGGCAAGAAGCTATTGTACTTGGAAGATCAACTTCACACAGCAATC
CACAGTCATGATGAAGATCTCATTCAGTCTCTCAGGAGGGAGCTCCAGAT
GGTGAAGGAAACTCTGCAGGCCATGATCCTGCAGCTCCAGCCAGCAAAGG
AGGCGGGAGAAAGAGAAGCTGCAGCTTCCTGCATGACAGCTGGTGTCCAC
GAAGCACAAGCCTGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHLGSPEFMPGGGPQGAPAAAGGGGVSHRAGSRDCLPPAACFRR
RRLARRPGYMRSSTGPGIGFLSPAVGTLFRFPGGVSGEESHHSESRARQC
GLDSRGLLVRSPVSKSAAAPTVTSVRGTSAHFGIQLRGGTRLPDRLSWPC
GPGSAGWQQEFAAMDSSETLDASWEAACSDGARRVRAAGSLPSAELSSNS
CSPGCGPEVPPTPPGSHSAFTSSFSFIRLSLGSAGERGEAEGCPPSREAE
SHCQSPQEMGAKAASLDGPHEDPRCLSQPFSLLATRVSADLAQAARNSSR
PERDMHSLPDMDPGSSSSLDPSLAGCGGDGSSGSGDAHSWDTLLRKWEPV
LRDCLLRNRRQMEVISLRLKLQKLQEDAVENDDYDKAETLQQRLEDLEQE
KISLHFQLPSRQPALSSFLGHLAAQVQAALRRGATQQASGDDTHTPLRME
PRLLEPTAQDSLHVSITRRDWLLQEKQQLQKEIEALQARMFVLEAKDQQL
RREIEEQEQQLQWQGCDLTPLVGQLSLGQLQEVSKALQDTLASAGQIPFH
AEPPETIRSLQERIKSLNLSLKEITTKVCMSEKFCSTLRKKVNDIETQLP
ALLEAKMHAISGNHFWTAKDLTEEIRSLTSEREGLEGLLSKLLVLSSRNV
KKLGSVKEDYNRLRREVEHQETAYETSVKENTMKYMETLKNKLCSCKCPL
LGKVWEADLEACRLLIQCLQLQEARGSLSVEDERQMDDLEGAAPPIPPRL
HSEDKRKTPLKVLEEWKTHLIPSLHCAGGEQKEESYILSAELGEKCEDIG
KKLLYLEDQLHTAIHSHDEDLIQSLRRELQMVKETLQAMILQLQPAKEAG
EREAAASCMTAGVHEAQA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11853
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct.