cDNA Clone Rad5 (yeast)

• Plasmid: pRS415 Pro-Rad5-cMyc
• Parent Plasmid: pRS415
• Tag: Myc
• Species: Yeast

Sequence of Insert

AAGCTTGTTCTAGATGCCTTTGAATTTGAAAAGCATGATGATCCCTTAAGGGATAAATGGAACACTTTACAGTTTTTGGAAAAGAGCTTTGAATCTAAATTTGAATCTGCATCAGAGTTAATCCAAGGAGGCGAGCTAGCTGCTATTAAAGAAAGAAATTTTCAATTAGCCAAACTAAATAATCTTTGCTTTCGGGTACGAGAGTCCATCAAAAGAAGACAAGACCTGGAGAAAAAGTTAAGAACTTTATCGCAGGACACTGACAACGAATTGCTTTTTCTGATGTTGGAAAATGAGAGAAGAAAG
AAAAGTTCAGTGATAATAGAGTTTCTTTCTGAGATAATCAGAGAGAAATC
CAAAAGATTAACTGCAGAAGAGCAAGGCTTTGTAAATCAAAATGAAGTAA
AACCCCTCATTCTGGACCTTTCTGCACGGATAAATAGATTGAATTCTATT
CTAGAAACAAAAAATACTTGTATTAGAAGATTAAGTAATCAATAAGTAGT
TAAATTTTCATAAATTATTACTTTCTTACATGTTAGTTGGCGCGTCGTCT
GGCTGAAAATTTTACGCGTCATAAACCCTTAACTTTTCCACACATTATTC
CTTACTGCTAAGCGCATTGCTCACTTGAAAGTAAATTATCTACAAAGTTA
CATTATCAAAAGGCCTTAGAAACACACCTAAAGTCTTACAGTATCACAAT
ATGAGTCATATTGAACAGGAAGAAAGGAAGAGGTTTTTTAACGATGACCT
TGACACTTCAGAAACATCGTTAAACTTCAAATCTGAGAATAAAGAGTCGT
TTTTATTTGCAAATAGTCATAATGATGATGATGATGATGTTGTTGTATCA
GTGAGTGATACAACAGAAGGAGAGGGAGACCGATCTATTGTGCCAGTCAG
GCGAGAAATAGAAGAAGAAGGCCAGAATCAGTTTATTACAGAGCTTTTAA
GGATTATTCCAGAAATGCCAAAGGATCTTGTGATGGAACTTAATGAAAAA
TTCGGCAGTCAGGAGGAAGGGCTCTCTTTAGCATTATCACATTACTTTGA
TCACAATAGCGGGACGTCTATCAGCAAAATACCGTCTTCCCCGAATCAAC
TAAATACACTCTCAGATACCTCAAATTCAACCTTGTCCCCATCTTCATTC
CATCCTAAAAGAAGAAGAATATACGGGTTCAGAAACCAAACACGATTAGA
AGATAAAGTTACTTGGAAAAGATTTATAGGTGCTTTGCAAGTCACTGGTA
TGGCTACCAGACCCACCGTCAGGCCCTTGAAGTACGGCTCTCAGATGAAG
CTAAAGAGATCAAGTGAAGAGATTTCTGCTACTAAAGTATATGACTCACG
TGGTAGAAAGAAAGCGTCCATGGCTAGTTTGGTAAGAATTTTTGATATCC
AATATGATAGAGAAATTGGCAGAGTTTCGGAAGACATTGCTCAAATACTA
TACCCTCTTTTAAGTTCACACGAAATAAGTTTTGAAGTTACATTGATTTT
TTGTGATAATAAACGGTTGAGTATAGGTGATAGCTTTATTCTACAATTGG
ATTGCTTTTTAACATCTCTCATTTTTGAGGAACGTAATGATGGAGAATCC
TTGATGAAAAGAAGACGTACAGAGGGAGGAAATAAAAGAGAGAAAGACAA
TGGAAATTTTGGAAGAACATTGACTGAAACTGATGAAGAGCTAGAAAGCC
GCTCGAAAAGACTGGCTCTACTAAAGTTATTTGATAAATTGAGGCTAAAG
CCTATTTTGGACGAGCAGAAGGCATTAGAAAAGCATAAAATAGAGCTTAA
TAGTGACCCCGAAATCATTGATTTAGATAACGACGAGATTTGCTCTAATC
AAGTGACTGAAGTCCATAACAATCTCCGAGATACTCAGCACGAAGAAGAA
ACAATGAACTTGAATCAATTGAAAACATTTTATAAGGCCGCACAATCATC
AGAATCTTTAAAAAGTTTGCCTGAAACAGAACCTTCTCGCGATGTCTTCA
AGCTAGAACTAAGAAATTATCAAAAGCAAGGTCTTACTTGGATGCTAAGG
AGGGAGCAAGAGTTTGCCAAAGCAGCCTCTGATGGTGAGGCTTCAGAAAC
GGGTGCTAATATGATAAACCCATTATGGAAGCAGTTCAAATGGCCAAATG
ATATGTCGTGGGCAGCTCAAAATTTGCAGCAGGACCATGTAAACGTTGAA
GATGGCATATTCTTTTATGCGAACTTACATTCTGGTGAATTTTCGCTAGC
AAAACCTATATTAAAAACTATGATAAAGGGTGGCATATTATCAGATGAAA
TGGGGTTGGGTAAAACAGTGGCAGCGTATTCTTTAGTTTTATCTTGTCCT
CACGATAGTGATGTTGTTGACAAGAAACTGTTTGATATTGAGAACACAGC
AGTCTCAGATAATCTTCCAAGCACTTGGCAAGATAATAAGAAACCATATG
CTTCAAAAACAACGCTAATCGTGGTCCCAATGTCTTTGTTAACGCAGTGG
AGTAACGAGTTTACAAAAGCTAATAATTCCCCCGATATGTATCATGAGGT
GTATTATGGTGGGAATGTTTCCAGTTTGAAAACCCTATTAACCAAGACAA
AAACCCCTCCAACTGTAGTCCTTACTACATATGGTATTGTTCAAAATGAA
TGGACTAAACATTCCAAGGGAAGGATGACAGATGAGGACGTCAATATATC
TTCAGGCTTATTTTCTGTCAATTTTTATCGCATAATAATCGACGAGGGTC
ATAACATTAGAAACAGAACGACAGTTACATCTAAAGCAGTCATGGCTTTA
CAAGGCAAATGTAAATGGGTTTTAACAGGAACACCAATTATTAACAGGCT
TGACGATTTATACAGTCTGGTTAAGTTTTTAGAGTTAGATCCCTGGCGGC
AAATTAATTACTGGAAGACCTTTGTATCAACGCCTTTTGAGAGTAAAAAT
TACAAACAAGCATTTGATGTGGTGAATGCAATTCTGGAACCCGTATTGTT
AAGAAGGACAAAACAAATGAAAGATAAAGATGGTAAGCCATTAGTAGAGT
TGCCACCAAAGGAGGTCGTTATTAAAAGACTTCCCTTCAGTAAATCTCAA
GATCTTCTATACAAGTTTCTGTTGGATAAGGCTGAAGTTTCTGTTAAATC
GGGTATTGCACGCGGTGATTTATTGAAAAAGTACTCCACTATCCTTGTCC
ATATTTTAAGATTGAGGCAAGTCTGTTGCCATCCCGGTCTTATTGGGAGT
CAAGATGAGAACGATGAGGATTTATCTAAAAATAATAAATTGGTTACGGA
ACAAACGGTGGAGCTTGACTCTTTAATGCGTGTTGTTTCCGAGAGATTCG
ATAACTCATTTTCTAAGGAGGAATTAGATGCAATGATACAAAGATTAAAA
GTTAAATATCCAGACAATAAATCGTTTCAGTCCTTAGAGTGCTCCATCTG
CACAACGGAACCTATGGATTTGGACAAGGCTTTATTTACAGAATGCGGCC
ACAGTTTTTGTGAGAAATGTTTATTTGAATATATTGAGTTTCAGAACAGT
AAGAATTTGGGTTTAAAGTGCCCCAATTGCCGTAACCAAATAGACGCTTG
TCGGTTGTTGGCATTGGTACAAACGAATAGCAACTCGAAAAATTTGGAAT
TCAAACCATATTCACCAGCCTCCAAATCAAGCAAAATCACTGCTTTATTG
AAGGAGCTTCAATTGCTACAGGATAGTTCGGCAGGCGAACAAGTTGTCAT
TTTTTCCCAATTTTCTACATACTTGGATATCCTGGAGAAAGAGCTAACTC
ATACTTTCTCAAAAGATGTTGCAAAAATTTATAAATTCGATGGACGTCTC
TCATTAAAAGAAAGAACTAGTGTATTAGCAGATTTTGCCGTTAAAGACTA
TAGCAGGCAAAAAATCCTATTACTCTCGCTGAAGGCTGGTGGCGTGGGTT
TGAATCTAACGTGTGCTTCCCACGCTTATATGATGGACCCATGGTGGTCG
CCCAGTATGGAAGATCAGGCAATCGATAGACTGCATAGAATTGGCCAGAC
AAACAGCGTCAAAGTTATGAGATTTATCATACAAGATAGCATAGAAGAAA
AAATGCTACGCATTCAAGAAAAGAAGAGAACCATCGGTGAGGCCATGGAC
ACAGACGAAGACGAGAGAAGAAAAAGGAGAATTGAAGAAATCCAGATGCT
GTTTGAAGAACAAAAACTTATTTCTGAAGAAGATCTGTAGCGGCCGC

Amino Acid Sequence

MSHIEQEERKRFFNDDLDTSETSLNFKSENKESFLFANSHNDDDDDVVVS
VSDTTEGEGDRSIVPVRREIEEEGQNQFITELLRIIPEMPKDLVMELNEK
FGSQEEGLSLALSHYFDHNSGTSISKIPSSPNQLNTLSDTSNSTLSPSSF
HPKRRRIYGFRNQTRLEDKVTWKRFIGALQVTGMATRPTVRPLKYGSQMK
LKRSSEEISATKVYDSRGRKKASMASLVRIFDIQYDREIGRVSEDIAQIL
YPLLSSHEISFEVTLIFCDNKRLSIGDSFILQLDCFLTSLIFEERNDGES
LMKRRRTEGGNKREKDNGNFGRTLTETDEELESRSKRLALLKLFDKLRLK
PILDEQKALEKHKIELNSDPEIIDLDNDEICSNQVTEVHNNLRDTQHEEE
TMNLNQLKTFYKAAQSSESLKSLPETEPSRDVFKLELRNYQKQGLTWMLR
REQEFAKAASDGEASETGANMINPLWKQFKWPNDMSWAAQNLQQDHVNVE
DGIFFYANLHSGEFSLAKPILKTMIKGGILSDEMGLGKTVAAYSLVLSCP
HDSDVVDKKLFDIENTAVSDNLPSTWQDNKKPYASKTTLIVVPMSLLTQW
SNEFTKANNSPDMYHEVYYGGNVSSLKTLLTKTKTPPTVVLTTYGIVQNE
WTKHSKGRMTDEDVNISSGLFSVNFYRIIIDEGHNIRNRTTVTSKAVMAL
QGKCKWVLTGTPIINRLDDLYSLVKFLELDPWRQINYWKTFVSTPFESKN
YKQAFDVVNAILEPVLLRRTKQMKDKDGKPLVELPPKEVVIKRLPFSKSQ
DLLYKFLLDKAEVSVKSGIARGDLLKKYSTILVHILRLRQVCCHPGLIGS
QDENDEDLSKNNKLVTEQTVELDSLMRVVSERFDNSFSKEELDAMIQRLK
VKYPDNKSFQSLECSICTTEPMDLDKALFTECGHSFCEKCLFEYIEFQNS
KNLGLKCPNCRNQIDACRLLALVQTNSNSKNLEFKPYSPASKSSKITALL
KELQLLQDSSAGEQVVIFSQFSTYLDILEKELTHTFSKDVAKIYKFDGRL
SLKERTSVLADFAVKDYSRQKILLLSLKAGGVGLNLTCASHAYMMDPWWS
PSMEDQAIDRLHRIGQTNSVKVMRFIIQDSIEEKMLRIQEKKRTIGEAMD
TDEDERRKRRIEEIQMLFEEQKLISEEDL*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11651
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee