cDNA Clone HDAC5

• Plasmid: pLZRSpBMN GFP-HDAC5
• Parent Plasmid: pLZRS BMN LacZ
• Expressed: GFP-HDAC5
• Genbank: NM_005474.4
• Tag: GFP
• Species: Human

Sequence of Insert

AAGCTTGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAAATCGATAACTCTCCCAACGAGTCGGATGGGATG
TCAGGTCGGGAACCATCCTTGGAAATCCTGCCGCGGACTTCTCTGCACAG
CATCCCTGTGACAGTGGAGGTGAAGCCGGTGCTGCCAAGAGCCATGCCCA
GTTCCATGGGGGGTGGGGGTGGAGGCAGCCCCAGCCCTGTGGAGCTACGG
GGGGCTCTGGTGGGCTCTGTGGACCCCACACTGCGGGAGCAGCAACTGCA
GCAGGAGCTCCTGGCGCTCAAGCAGCAGCAGCAGCTGCAGAAGCAGCTCC
TGTTCGCTGAGTTCCAGAAACAGCATGACCACCTGACAAGGCAGCATGAG
GTCCAGCTGCAGAAGCACCTCAAGCAGCAGCAGGAGATGCTGGCAGCCAA
GCAGCAGCAGGAGATGCTGGCAGCCAAGCGGCAGCAGGAGCTGGAGCAGC
AGCGGCAGCGGGAGCAGCAGCGGCAGGAAGAGCTGGAGAAGCAGCGGCTG
GAGCAGCAGCTGCTCATCCTGCGGAACAAGGAGAAGAGCAAAGAGAGTGC
CATTGCCAGCACTGAGGTAAAGCTGAGGCTCCAGGAATTCCTCTTGTCGA
AGTCAAAGGAGCCCACACCAGGCGGCCTCAACCATTCCCTCCCACAGCAC
CCCAAATGCTGGGGAGCCCACCATGCTTCTTTGGACCAGAGTTCCCCTCC
CCAGAGCGGCCCCCCTGGGACGCCTCCCTCCTACAAACTGCCTTTGCCTG
GGCCCTACGACAGTCGAGACGACTTCCCCCTCCGCAAAACAGCCTCTGAA
CCCAACTTGAAAGTGCGTTCAAGGCTAAAACAGAAGGTGGCTGAGCGGAG
AAGCAGTCCCCTCCTGCGTCGCAAGGATGGGACTGTTATTAGCACCTTTA
AGAAGAGAGCTGTTGAGATCACAGGTGCCGGGCCTGGGGCGTCGTCCGTG
TGTAACAGCGCACCCGGCTCCGGCCCCAGCTCTCCCAACAGCTCCCACAG
CACCATCGCTGAGAATGGCTTTACTGGCTCAGTCCCCAACATCCCCACTG
AGATGCTCCCTCAGCACCGAGCCCTCCCTCTGGACAGCTCCCCCAACCAG
TTCAGCCTCTACACGTCTCCTTCTCTGCCCAACATCTCCCTAGGGCTGCA
GGCCACGGTCACTGTCACCAACTCACACCTCACTGCCTCCCCGAAGCTGT
CGACACAGCAGGAGGCCGAGAGGCAGGCCCTCCAGTCCCTGCGGCAGGGT
GGCACGCTGACCGGCAAGTTCATGAGCACATCCTCTATTCCTGGCTGCCT
GCTGGGCGTGGCACTGGAGGGCGACGGGAGCCCCCACGGGCATGCCTCCC
TGCTGCAGCATGTGCTGTTGCTGGAGCAGGCCCGGCAGCAGAGCACCCTC
ATTGCTGTGCCACTCCACGGGCAGTCCCCACTAGTGACGGGTGAACGTGT
GGCCACCAGCATGCGGACGGTAGGCAAGCTCCCGCGGCATCGGCCCCTGA
GCCGCACTCAGTCCTCACCGCTGCCGCAGAGTCCCCAGGCCCTGCAGCAG
CTGGTCATGCAACAACAGCACCAGCAGTTCCTGGAGAAGCAGAAGCAGCA
GCAGCTACAGCTGGGCAAGATCCTCACCAAGACAGGGGAGCTGCCCAGGC
AGCCCACCACCCACCCTGAGGAGACAGAGGAGGAGCTGACGGAGCAGCAG
GAGGTCTTGCTGGGGGAGGGAGCCCTGACCATGCCCCGGGAGGGCTCCAC
AGAGAGTGAGAGCACACAGGAAGACCTGGAGGAGGAGGACGAGGAAGAGG
ATGGGGAGGAGGAGGAGGATTGCATCCAGGTTAAGGACGAGGAGGGCGAG
AGTGGTGCTGAGGAGGGGCCCGACTTGGAGGAGCCTGGTGCTGGATACAA
AAAACTGTTCTCAGATGCCCAGCCGCTGCAGCCTTTGCAGGTGTACCAGG
CGCCCCTCAGCCTGGCCACTGTGCCCCACCAGGCCCTGGGCCGTACCCAG
TCCTCCCCTGCTGCCCCTGGGGGCATGAAGAGCCCCCCAGACCAGCCCGT
CAAGCACCTCTTCACCACAGGTGTGGTCTACGACACGTTCATGCTAAAGC
ACCAGTGCATGTGCGGGAACACACACGTGCACCCTGAGCATGCTGGCCGG
ATCCAGAGCATCTGGTCCCGGCTGCAGGAGACAGGCCTGCTTAGCAAGTG
CGAGCGGATCCGAGGTCGCAAAGCCACGCTAGATGAGATCCAGACAGTGC
ACTCTGAATACCACACCCTGCTCTATGGGACCAGTCCCCTCAACCGGCAG
AAGCTAGACAGCAAGAAGTTGCTCGGCCCCATCAGCCAGAAGATGTATGC
TGTGCTGCCTTGTGGGGGCATCGGGGTGGACAGTGACACCGTGTGGAATG
AGATGCACTCCTCCAGTGCTGTGCGCATGGCAGTGGGCTGCCTGCTGGAG
CTGGCCTTCAAGGTGGCTGCAGGAGAGCTCAAGAATGGATTTGCCATCAT
CCGGCCCCCAGGACACCACGCCGAGGAATCCACAGCCATGGGATTCTGCT
TCTTCAACTCTGTAGCCATCACCGCAAAACTCCTACAGCAGAAGTTGAAC
GTGGGCAAGGTCCTCATCGTGGACTGGGACATTCACCATGGCAATGGCAC
CCAGCAGGCGTTCTATAATGACCCCTCTGTGCTCTACATCTCTCTGCATC
GCTATGACAACGGGAACTTCTTTCCAGGCTCTGGGGCTCCTGAAGAGGTT
GGTGGAGGACCAGGCGTGGGGTACAATGTGAACGTGGCATGGACAGGAGG
TGTGGACCCCCCCATTGGAGACGTGGAGTACCTTACAGCCTTCAGGACAG
TGGTGATGCCCATTGCCCACGAGTTCTCACCTGATGTGGTCCTAGTCTCC
GCCGGGTTTGATGCTGTTGAAGGACATCTGTCTCCTCTGGGTGGCTACTC
TGTCACCGCCAGATGTTTTGGCCACTTGACCAGGCAGCTGATGACCCTGG
CAGGGGGCCGGGTGGTGCTGGCCCTGGAGGGAGGCCATGACTTGACCGCC
ATCTGTGATGCCTCTGAGGCTTGTGTCTCGGCTCTGCTCAGTGTAGAGCT
GCAGCCCTTGGATGAGGCAGTCTTGCAGCAAAAGCCCAACATCAACGCAG
TGGCCACGCTAGAGAAAGTCATCGAGATCCAGAGCAAACACTGGAGCTGT
GTGCAGAAGTTCGCCGCTGGTCTGGGCCGGTCCCTGCGAGAGGCCCAAGC
AGGTGAGACCGAGGAGGCCGAGACTGTGAGCGCCATGGCCTTGCTGTCGG
TGGGGGCCGAGCAGGCCCAGGCTGCGGCAGCCCGGGAACACAGCCCCAGG
CCGGCAGAGGAGCCCATGGAGCAGGAGCCTGCCCTGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKIDNSPNESDGM
SGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELR
GALVGSVDPTLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHE
VQLQKHLKQQQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRL
EQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQH
PKCWGAHHASLDQSSPPQSGPPGTPPSYKLPLPGPYDSRDDFPLRKTASE
PNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEITGAGPGASSV
CNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQ
FSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQG
GTLTGKFMSTSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTL
IAVPLHGQSPLVTGERVATSMRTVGKLPRHRPLSRTQSSPLPQSPQALQQ
LVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQ
EVLLGEGALTMPREGSTESESTQEDLEEEDEEEDGEEEEDCIQVKDEEGE
SGAEEGPDLEEPGAGYKKLFSDAQPLQPLQVYQAPLSLATVPHQALGRTQ
SSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGR
IQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQ
KLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLE
LAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLN
VGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEV
GGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVS
AGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTA
ICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSC
VQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPR
PAEEPMEQEPAL*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11529
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Retroviral expression vector. Ex Doreen Cantrell’s lab.; Sequence differences compared with NM_005474.4 CDS:; c2499g (D593E in HDAC5); c3366t (silent); (originate from DU11456); ; Estimated MW of expressed protein : 149007 Daltons