cDNA Clone Mms1 (yeast)

• Plasmid: pRS416 Pro-Mms1 (ATG=Nco1)
• Parent Plasmid: pRS416
• Genbank: NC_001148
• Species: Yeast

Sequence of Insert

GCGGCCGCTCGAGTTTTATGATAATCCCGCTTATTGGTCTACTGATCTTCTCTAACTCTGTAGTTATATCTATCTCTTCTGCTTCTATTTCTGCTTTTCTTTAGCGATGCTGATTTCCGCAAAAAAATTTTCATTCCGTGGTGCGTGATCTGATGAGAAATAAAGCCAAAAAGAAAAAAAGACAAGAAGACAAAAAGGTTGGGCGGCTAAGTAGCGCCAGGATATAAAAAGCAACATAGATTTCAGTACCCTATTATCCTGCCAAAATTTACTTTAAGAAGTCTCTATGAATATACAGGCTAAATTTGGAAACATGAGAAAGGAAAGATGAAGATACAAAGATTGCAAGCAGTCAAG
AAGATTTAGGAAAAAAGGAAAAAGCAAAAAATATCGATTTTTATGACTTA
CAAACTAAATTGATATGTTCGAAATAATTTTGAGAAGCTGAGCTCAATCT
GGTACTAATCACTTCAAGTTAGATGTGCAAAGAAAGAGAATAGCATAAAT
TCAAAACCAAAGGAAGTGCGAGGCATTACCCGCATATTATCGGGAAACAG
AAGCCATGTTAGAGTGATTTCCAACAAATGGCCCTTTGCCAGAGATGGGC
TCTGCAAAAGAATTGGAAGAAGAGAAGAAATCGCTTCACATTCCGTTTCG
TTTCTGGGTCTAATTAAAGTAGTACGGTGTCAATTAGCCATGGTAGGTTT
GCGAACTCATGGTTTAGACAGGTATGAACATTATATTCGTCGCCCGTCGG
ATTTTGGCAAACTTGAGCTGCAAGATTGGTTGAATCATAAGTCATTCCGA
GTTTCCCCCAATTTATTAATTGATTCAAGCACCACACGTGAGTGGAATGA
ACCTGAGCTTTTTTATCAAAATACCGAGGATGAAACTTGGGTGAGACCTT
GTGTAGGGCCAAAGCTAGAACCATCAATGATGATGCTCAGATATCACGAT
TCGAATATTGGTCAAATGCCTCAATTTTGTTATCCAATCTCAAGTCCGAT
AAATTTTAAACCAGTATTAAAATACATTTTGCAAGAACGTTCTGAACTGT
CAGACGGCTTCCCTCAAAAGTATAACACACTAATAGGTAGTCTTTTTGAC
ATTGATAAAAACCCAGAAACCTTAGATGATTCAGATATAGAAGCATTGGA
TGACATAGAAATGAGCAGTGACAGCGGTAATGTTAAAGAACCAAAAATTG
AATTGCAGGCGCTGGAAGAAATCCAACAAAAGCATTTCAGTTTAATAGTA
TCCAACAATGGAATCTTTCAAACAGGTAGCACTTCAATAACATACATACA
GTCTGGCATATCTGGCAGCATAGCTATAAAACCCAACAACGTTGCAATTT
TAATATTACTCACTCAACCAAGTGGTCACTTATTGTCTATTTTACCGCTT
GATGACGGTAAAGAGACATATTTGCTACAATATTGGAACCTGGGACAAAA
AGGTCAGTGGAACATAATCAAGCACCAAAACGAGAAGCAGTTTGTTCTTA
TACATAAGGAACTAGGCATTTGCAAATTTTTTGAATTCCATTTACCATTT
ACTTTTCAATTAGTAAACAATTTAACATTGACCGATTCCGTGATTATGAA
CGGATCCTTTTTCCCAACAAATTACACTGATTTAGATCCCTATTTCATTA
TATTTATAACAGCCATAAGATATGAAAGGATAGTCTACTTTGTCATAGAA
TGGAACAACAACGAAATAAAGAAAAAAGAGGTATATCAATTGACAGTATT
TGATGGTGAGAAGACTAATATGACAATACCCATTGGACTAAATGCATGTT
TAGTCGAAACACCCCTAAAGTTCTCTTTAGTTTCTGCAAATCAAATTATG
TCAGGAGAGACTGAATTCCACTCATTCCAATTGAAGGCTCTCAAGGGAAT
CAAGTCATTTTTTCCAGCTCCTTTATTGTTATTAAAATTACAAGAACTAC
ACCCACATACATTTAAAAAATTCCAATATTGTACCATAATATCCTCCTCG
ACAGGAAATATTTGTTTTTGCGTCACCGAACGATCGACAATAGTAAATGG
TAATTTAAAATTTTACGAGCTAACTAGGTTCAAAGGATTGAAATCCATTT
CACCACTACCTTCAAATCCGATAAATTTAGACTCCAGATCCTCAAGTTAT
GTACTGGTGGTCATAAGTTTTAGTAGAACATTAGAGCTAACATTATCTCT
AGAAGATTTGAGATGTTTAGATAAAAAAGACGTTATTAAGCCTTTGAAAA
ATATCACCTTCAAGCACACAATTGATAGTTCCACAGAGGAGAACTCTCAA
ATTTTAGCATTTACGTCTTCTAAATTTTATAACACACACACAGGCTCTAA
CATCAATGACACGAGAAATTCTCAAGTTTGGCTTACCTCACCAAATGCAA
TAACTCAACCTTGCATTGATTATAAACTCAGGAAAACTCATCAACTTATC
CACTTAAAGCAATTTCAAATTTTTAGACATCTTAGGATATGGAAATGTAA
GAACCTTGATATTGCTCTGTTACAGAGACTTGGAATAAACCAGTCAAATA
CCGAAAGTTCGTTAATTTTTGCGACCGACGCTGTTTCTAACAACAGAATA
TTTTTATTAGATTTAACTATGACAACGACAATCGATAATGATGATCCCGT
TCAAGGACTGATAAATATAGAAGATTTACTATGTGATACTGAAAACGAAA
CTATCCTTCTAAATTTTACGAAAAATAACTTGATTCAAGTAACTAGGGAT
ACGATATACATCGATCCCATTGGTGGGGACAAAGAGTTGCGTAAGATTTC
TCCAGGTTGGGAATTCGAAAACGTTACATACAATGATGGTATTTTAATAG
TTTGGAATGCTGGGCTTGGCTGTGTCTCTTATATTGAAAATATAGACGCT
GTTGATGAGTCTGGCGCCTTAGTTTCAAACTTAAGCAGCAGCAAAGGCAT
GAGCAAGTTCTTCAAGCAGTTAGGAACTGTCACGAGTGTCAATTTTCAAA
TCAAAGAGTCTACAGATGATCCAACCAAGTATGACATTTGGATCCTTTTA
CCAGACTGTGTCATTCGTACACCCTTTTCTGACTGGATTAGTGATTCACT
TGATTTTTCTGATGTGTATATTTTGAGTGTTCAGCAGGCGTTAATAAACG
GCCCTTATTTTTGCTCTCTCGATTATGAATCATATTTTGAGGTGCACACT
TTACAGAACAACTGCTTCAAAAAAGGATCCAGATGTACAAGCAGAGTTAA
TTTTCAAGGGAAAGATATTAAATTTAGAAGCTTTGGTGTGAATCAATGTT
TGGCATTTAGTGCATTTGAAATTTTTGTCATCAATTTAACGCCAATTCAC
GACAGTAGAGAATTGGATTTTTACAAGCTAAAATTACCGCACCTAGGTAA
TAACAATTCAATTCTTGAAGTCTGTCCGGACATAGAAAACAACCAATTAT
TCATACTCTACTCTGATGGCTTAAGAATCCTCGAACTATCATACCTAACA
TCAAATAATGGAAATTTCTTATTAAAATCTACAAGAAGCAAAAATAAGAA
ATTTTTATATCTAGACAAAATAAATCGAATGCTGGTATTGAATCAGGACT
TGAGGGAATGGGAGTGTATTAGACTATCAGATGGTAAGGCAGTTGGTTTA
GATTCTCAACTTCTTAAGGATGATTCTGAAGAAATCCTAGAAATAAAGGA
ATTACCAATAGCAACAGAGGACAATCCTTTAGAAAAGAAAACTGTATTAT
TGATCTCTTTTACTAGCTCATTAAAACTAGTTTTATTAACTGCTGCAAAA
AACAAAATTTCCAATCAAATAATAGATTCGTATAAACTTGACAATTCAAG
ACTCCTCAATCATTTGGTCATTACTCCCAGAGGTGAAATATTCTTTCTGG
ATTATAAAGTTATGGGCACCGATAACGAAATGTCCTTTAACAAGTTGAAA
GTCACAAAACATTGCATTGACCAGGAGGAGAGAAATAATACGACTTTGCG
GCTCACTTTGGAAACCCGGTTTACATTTAAGAGTTGGAGTACAGTTAAGA
CGTTTACTGTAGTAGGCGATAATATCATTGCTACCACAAATATGGGCGAA
AAGCTCTACTTGATTAAGGATTTCTCTTCATCATCTGACGAATCGAGAAG
GGTGTATCCCTTGGAAATGTATCCTGATTCAAAAGTTCAAAAGATAATAC
CATTAAATGAATGCTGCTTTGTTGTTGCCGCTTACTGTGGAAATAGGAAC
GATTTAGATTCAAGATTAATTTTTTACTCTTTACCCACCATCAAAGTTGG
GCTTAATAACGAAACAGGCAGCCTACCAGATGAATATGGGAATGGGAGAG
TCGACGACATATTCGAGGTTGACTTTCCTGAAGGATTTCAATTTGGCACT
ATGGCTTTGTATGATGTTTTACATGGTGAGAGGCACGTAAATCGTTACAG
CGAAGGCATACGGTCGGAGAATGACGAAGCAGAGGTTGCCCTAAGGCAGC
GTAGAAATTTACTACTCTTTTGGCGAAACCACTCTTCTACACCAAAACCT
TCACTACGCCGAGCCGCCACTATAGTATATGAGGATCATGTATCATCCCG
TTATTTTGAGGATATAAGTTCTATATTAGGAAGCACTGCAATGAGAACTA
AAAGACTATCTCCCTATAATGCGGTAGCATTGGACAAGCCTATTCAAGAT
ATTAGTTACGATCCCGCAGTACAAACTTTATATGTGCTAATGGCAGATCA
AACAATTCACAAATTCGGCAAGGACAGGTTGCCTTGCCAGGACGAATACG
AACCAAGATGGAATTCTGGCTATTTGGTTTCAAGAAGGTCAATAGTTAAA
TCTGACCTCATCTGTGAGGTTGGGTTATGGAACCTTAGCGATAACTGCAA
GAACACAGTATAACCGCGG

Amino Acid Sequence

MVGLRTHGLDRYEHYIRRPSDFGKLELQDWLNHKSFRVSPNLLIDSSTTR
EWNEPELFYQNTEDETWVRPCVGPKLEPSMMMLRYHDSNIGQMPQFCYPI
SSPINFKPVLKYILQERSELSDGFPQKYNTLIGSLFDIDKNPETLDDSDI
EALDDIEMSSDSGNVKEPKIELQALEEIQQKHFSLIVSNNGIFQTGSTSI
TYIQSGISGSIAIKPNNVAILILLTQPSGHLLSILPLDDGKETYLLQYWN
LGQKGQWNIIKHQNEKQFVLIHKELGICKFFEFHLPFTFQLVNNLTLTDS
VIMNGSFFPTNYTDLDPYFIIFITAIRYERIVYFVIEWNNNEIKKKEVYQ
LTVFDGEKTNMTIPIGLNACLVETPLKFSLVSANQIMSGETEFHSFQLKA
LKGIKSFFPAPLLLLKLQELHPHTFKKFQYCTIISSSTGNICFCVTERST
IVNGNLKFYELTRFKGLKSISPLPSNPINLDSRSSSYVLVVISFSRTLEL
TLSLEDLRCLDKKDVIKPLKNITFKHTIDSSTEENSQILAFTSSKFYNTH
TGSNINDTRNSQVWLTSPNAITQPCIDYKLRKTHQLIHLKQFQIFRHLRI
WKCKNLDIALLQRLGINQSNTESSLIFATDAVSNNRIFLLDLTMTTTIDN
DDPVQGLINIEDLLCDTENETILLNFTKNNLIQVTRDTIYIDPIGGDKEL
RKISPGWEFENVTYNDGILIVWNAGLGCVSYIENIDAVDESGALVSNLSS
SKGMSKFFKQLGTVTSVNFQIKESTDDPTKYDIWILLPDCVIRTPFSDWI
SDSLDFSDVYILSVQQALINGPYFCSLDYESYFEVHTLQNNCFKKGSRCT
SRVNFQGKDIKFRSFGVNQCLAFSAFEIFVINLTPIHDSRELDFYKLKLP
HLGNNNSILEVCPDIENNQLFILYSDGLRILELSYLTSNNGNFLLKSTRS
KNKKFLYLDKINRMLVLNQDLREWECIRLSDGKAVGLDSQLLKDDSEEIL
EIKELPIATEDNPLEKKTVLLISFTSSLKLVLLTAAKNKISNQIIDSYKL
DNSRLLNHLVITPRGEIFFLDYKVMGTDNEMSFNKLKVTKHCIDQEERNN
TTLRLTLETRFTFKSWSTVKTFTVVGDNIIATTNMGEKLYLIKDFSSSSD
ESRRVYPLEMYPDSKVQKIIPLNECCFVVAAYCGNRNDLDSRLIFYSLPT
IKVGLNNETGSLPDEYGNGRVDDIFEVDFPEGFQFGTMALYDVLHGERHV
NRYSEGIRSENDEAEVALRQRRNLLLFWRNHSSTPKPSLRRAATIVYEDH
VSSRYFEDISSILGSTAMRTKRLSPYNAVALDKPIQDISYDPAVQTLYVL
MADQTIHKFGKDRLPCQDEYEPRWNSGYLVSRRSIVKSDLICEVGLWNLS
DNCKNTV*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11491
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for untagged Mms1.; L2V due to Nco1 site.