cDNA Clone Flotillin 1

• Plasmid: pGADT7 Flotillin 1
• Parent Plasmid: pGADT7
• Expressed: GAL4-AD Flotillin
• Genbank: NM_005803.2 GI:6552331
• Tag: GAL4 DNA-AD
• Species: Human

Sequence of Insert

gaattcatgtttttcacttgtggcccaaatgaggccatggtggtctccgggttctgccgaagccccccagtcatggtggctggagggcgtgtctttgtcctgccctgcatccaacagatccagaggatctctctcaacacactgaccctcaatgtcaagagtgaaaaggtttacactcgccatggggtccccatctcagtcactggcattgcccaggtaaaaatccaggggcagaacaaggagatgttggcggccgcctgtcagatgttcctggggaagacggaggctgagattgcccacattgcc
ctggagacgttagagggccaccagagggccatcatggcccacatgactgt
ggaggagatctataaggacaggcagaaattctcagaacaggttttcaaag
tggcctcctcagacctggtcaacatgggcatcagtgtggttagctacact
ctgaaggacattcacgatgaccaggactatttgcactctttggggaaggc
tcgaacagctcaagtccaaaaagatgcacggattggagaagcagaggcca
agagagatgctgggatccgggaagctaaagccaagcaggaaaaggtgtct
gctcagtacctgagtgagatcgagatggccaaggcacagagagattacga
actgaagaaggccgcctatgacatcgaggtcaacacccgccgagcacagg
ctgacctggcctatcagcttcaggtggccaagactaagcagcagattgag
gagcagcgggtgcaggtgcaggtggtggagcgggcccagcaggtggcagt
gcaggagcaggagatcgcccggcgggagaaggagctggaggcccgggtgc
ggaagccagcggaagcggagcgctacaagctggagcgcctagccgaggca
gagaagtcccaactaattatgcaggcggaggcagaagccgcgtctgtgcg
gatgcgtggggaagctgaggcctttgccataggggcccgagcccgagccg
aggctgagcagatggccaagaaggcagaagccttccagctgtaccaagag
gctgctcagctggacatgctgctagagaagctgccccaggtggcagagga
gatcagtggtcccttgacttcagccaataagatcacactggtgtccagcg
gcagtgggaccatgggggcagccaaagtgactggggaagtactggacatt
ctaactcgcctgccagagagtgtggaaagactcacaggcgtgagcatctc
ccaggtgaatcacaagcctttgagaacagcctgactcgag

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLI
TTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAAME
YPYDVPDYAHMAMEASEFMFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVL
PCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQVKIQGQNKEMLA
AACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVF
KVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAE
AKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRA
QADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEAR
VRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARAR
AEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVS
SGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11459
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clontech MATCHMAKER vector for yeast Two-Hybrid screen.