cDNA Clone Flotillin 1

• Plasmid: pCMV5-FLAG flotillin 1
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG flotillin
• Genbank: NM_005803.2 GI:6552331
• Tag: FLAG
• Species: Human

Sequence of Insert

gaattcatgtttttcacttgtggcccaaatgaggccatggtggtctccgggttctgccgaagccccccagtcatggtggctggagggcgtgtctttgtcctgccctgcatccaacagatccagaggatctctctcaacacactgaccctcaatgtcaagagtgaaaaggtttacactcgccatggggtccccatctcagtcactggcattgcccaggtaaaaatccaggggcagaacaaggagatgttggcggccgcctgtcagatgttcctggggaagacggaggctgagattgcccacattgcc
ctggagacgttagagggccaccagagggccatcatggcccacatgactgt
ggaggagatctataaggacaggcagaaattctcagaacaggttttcaaag
tggcctcctcagacctggtcaacatgggcatcagtgtggttagctacact
ctgaaggacattcacgatgaccaggactatttgcactctttggggaaggc
tcgaacagctcaagtccaaaaagatgcacggattggagaagcagaggcca
agagagatgctgggatccgggaagctaaagccaagcaggaaaaggtgtct
gctcagtacctgagtgagatcgagatggccaaggcacagagagattacga
actgaagaaggccgcctatgacatcgaggtcaacacccgccgagcacagg
ctgacctggcctatcagcttcaggtggccaagactaagcagcagattgag
gagcagcgggtgcaggtgcaggtggtggagcgggcccagcaggtggcagt
gcaggagcaggagatcgcccggcgggagaaggagctggaggcccgggtgc
ggaagccagcggaagcggagcgctacaagctggagcgcctagccgaggca
gagaagtcccaactaattatgcaggcggaggcagaagccgcgtctgtgcg
gatgcgtggggaagctgaggcctttgccataggggcccgagcccgagccg
aggctgagcagatggccaagaaggcagaagccttccagctgtaccaagag
gctgctcagctggacatgctgctagagaagctgccccaggtggcagagga
gatcagtggtcccttgacttcagccaataagatcacactggtgtccagcg
gcagtgggaccatgggggcagccaaagtgactggggaagtactggacatt
ctaactcgcctgccagagagtgtggaaagactcacaggcgtgagcatctc
ccaggtgaatcacaagcctttgagaacagcctgagaattc

Amino Acid Sequence

MDYKDDDDKGSPEFMFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQ
QIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQVKIQGQNKEMLAAACQ
MFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVAS
SDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRD
AGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADL
AYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKP
AEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAE
QMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSG
TMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11438
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 48995 Daltons