cDNA Clone DPB2 (yeast)

• Plasmid: pRS415 DPB2E
• Parent Plasmid: pRS415
• Species: Yeast

Sequence of Insert

GGATCCCTCAATATTTTTCATGTCTCGACGAGACTCCAGGTATCTTTGATCGATGTTTGCGTCGTCCAACACCGACTGCAACAGATGACACTTTTCCTTGACAAATTGTAGTTCCTTTTGTGTAGAGTGCAGCTGCTGCCGCAATTGCGCAAACTTTGCTTGCCATTGTAGAGGCACACAATCCTGAGAATTTTTAGACAATGGGACAACGCTTTCTTCTTCCTCATCTGCGGGAGACCCCCATACAAACGCTTGGATCTTGGCAAGAGTCTTAGCAATATTGAATCCAAACGCGTGTTGTTGAGC
CCTCTTTTTGGAGATATTCTTGTAGTTTTTAGAACGTGTCCTTTGTGAAG
CGTGTCTAACATGATTTCTTTCGCGAGGCACAACCACCAATTTGCGCTCT
TTTCGTATCCCCAATGTCTTTTCCTGAATTCCCATTGTGATCTTCAATGC
CAACCCAACCTTCTTTTTACTTTGTTGGGTCTAAATTATGTTACTTTGTC
CATTTCATCTCTTTTCAAGATGGAATCTAAAAGAAGAAAAACGACAAAAG
GAACAAAAGTGATGTGACGCGTTATTTTTCGCGTGACATAATAGAAAAAT
ATTTCAACTGGGCATCTTTAACACATGTTACAATAAAGTTGAGATGACTT
GTTTATGCCTTTAAAGTTTGTAAGTGGAACTAGAAGCGTCTATGTGTGAA
ATGTTTGGCTCTGGGAATGTTCTGCCTGTTAAAATTCAGCCTCCGCTGCT
TAGGCCACTGGCGTATAGAGTTTTATCAAGAAAATATGGTTTATCAATTA
AATCTGATGGACTGTCTGCTCTTGCAGAATTCGTTGGCACTAATATAGGT
GCTAACTGGAGACAAGGGCCTGCTACAATAAAATTTCTCGAGCAATTTGC
TGCAGTATGGAAACAACAAGAAAGAGGCCTTTTCATCGACCAGAGCGGGG
TTAAAGAAGTTATACAGGAGATGAAAGAACGTGAGAAAGTCGAATGGAGT
CATGAACACCCTATTCAACATGAGGAGAATATCTTGGGACGGACAGATGA
CGATGAGAACAACAGTGACGATGAAATGCCGATCGCTGCTGATTCATCCT
TACAAAATGTTTCATTATCTTCACCCATGCGACAGCCCACCGAAAGAGAT
GAATATAAACAGCCTTTCAAGCCAGAAAGCTCCAAGGCGTTGGACTGGAG
GGACTACTTCAAAGTTATCAATGCTTCTCAGCAACAAAGATTCTCATACA
ATCCGCACAAAATGCAATTTATTTTCGTCCCCAATAAAAAGCAGAATGGG
CTAGGAGGCATTGCGGGTTTTCTACCAGATATAGAGGACAAAGTTCAAAT
GTTCTTGACAAGATATTACCTCACGAACGACAGAGTCATGAGAAATGAAA
ACTTCCAAAACAGTGACATGTTTAATCCATTATCATCTATGGTGTCTTTA
CAAAATGAACTGTCCAATACTAACCGACAGCAGCAATCCAGCAGTATGAG
TATCACCCCAATAAAAAACCTACTAGGTAGGGATGCTCAAAACTTTCTAC
TATTGGGGCTCTTAAATAAGAACTTTAAGGGCAATTGGTCACTGGAAGAT
CCGTCTGGATCTGTCGAAATTGACATTTCCCAAACTATCCCTACACAAGG
CCATTACTACGTGCCAGGTTGCATGGTTCTTGTGGAAGGAATATACTATT
CTGTAGGAAATAAATTCCATGTTACCTCCATGACTTTACCCCCTGGTGAG
AGAAGAGAAATTACATTAGAAACGATAGGTAATCTCGATCTCTTAGGAAT
ACATGGCATTTCTAATAACAATTTCATTGCTCGTTTGGATAAAGATTTGA
AGATTAGATTACACCTTTTGGAGAAAGAATTAACAGATCATAAATTTGTA
ATTCTCGGCGCAAATTTGTTCCTAGATGATTTGAAAATTATGACTGCACT
CAGCAAAATTTTGCAAAAATTAAATGATGACCCACCGACCCTATTAATTT
GGCAAGGTTCTTTCACTTCAGTTCCCGTTTTCGCATCAATGAGTAGCCGG
AATATAAGTAGTTCCACTCAATTTAAGAATAATTTCGATGCCTTGGCGAC
GCTTCTGTCAAGATTTGACAATTTGACCGAAAATACCACAATGATATTTA
TTCCAGGTCCTAACGATTTATGGGGGTCGATGGTGTCATTGGGGGCAAGT
GGGACATTACCGCAAGATCCAATTCCTAGTGCGTTTACCAAAAAAATCAA
CAAGGTCTGTAAAAACGTTGTATGGAGCTCAAATCCAACTAGAATAGCAT
ACTTATCCCAAGAAATAGTCATTTTCAGGGACGATTTATCCGGAAGATTC
AAAAGACACCGTTTGGAATTCCCATTCAACGAGAGCGAAGATGTTTATAC
TGAAAACGATAACATGATGTCTAAAGACACCGATATTGTACCAATCGATG
AATTAGTTAAAGAACCAGACCAGTTACCACAAAAGGTTCAAGAAACAAGA
AAACTCGTTAAAACAATACTAGACCAGGGTCATTTATCGCCATTTCTTGA
TTCCTTGCGCCCAATTTCATGGGATTTGGACCACACTTTGACACTTTGCC
CAATACCATCGACAATGGTTCTTTGCGACACTACTTCTGCACAATTTGAT
TTGACATACAACGGTTGTAAAGTAATTAACCCTGGAAGTTTCATTCATAA
TAGACGTGCTAGGTATATGGAGTACGTTCCATCCTCAAAGAAAACTATAC
AAGAAGAGATATATATCTAAATAATGCATCAAAATAAGAACACTTAAACA
AGCACGGTAGTGTTGGTACTCTGCCATCTATTTGTATGGATACTTTTTGA
ATTTTGATGTAACGGATTTCGCGGCCGC

Amino Acid Sequence

MFGSGNVLPVKIQPPLLRPLAYRVLSRKYGLSIKSDGLSALAEFVGTNIG
ANWRQGPATIKFLEQFAAVWKQQERGLFIDQSGVKEVIQEMKEREKVEWS
HEHPIQHEENILGRTDDDENNSDDEMPIAADSSLQNVSLSSPMRQPTERD
EYKQPFKPESSKALDWRDYFKVINASQQQRFSYNPHKMQFIFVPNKKQNG
LGGIAGFLPDIEDKVQMFLTRYYLTNDRVMRNENFQNSDMFNPLSSMVSL
QNELSNTNRQQQSSSMSITPIKNLLGRDAQNFLLLGLLNKNFKGNWSLED
PSGSVEIDISQTIPTQGHYYVPGCMVLVEGIYYSVGNKFHVTSMTLPPGE
RREITLETIGNLDLLGIHGISNNNFIARLDKDLKIRLHLLEKELTDHKFV
ILGANLFLDDLKIMTALSKILQKLNDDPPTLLIWQGSFTSVPVFASMSSR
NISSSTQFKNNFDALATLLSRFDNLTENTTMIFIPGPNDLWGSMVSLGAS
GTLPQDPIPSAFTKKINKVCKNVVWSSNPTRIAYLSQEIVIFRDDLSGRF
KRHRLEFPFNESEDVYTENDNMMSKDTDIVPIDELVKEPDQLPQKVQETR
KLVKTILDQGHLSPFLDSLRPISWDLDHTLTLCPIPSTMVLCDTTSAQFD
LTYNGCKVINPGSFIHNRRARYMEYVPSSKKTIQEEIYI*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11395
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for untagged DPB2.; Differences from ref sequence:; t2073a (F458Y), a2633c (T645P)