cDNA Clone Mms1 (yeast)

• Plasmid: pGEX6P-1-Mms1-6His
• Parent Plasmid: pGEX6P1
• Tag: 6His
• Species: Yeast

Sequence of Insert

GCGGCCGCATATGCTAGGTTTGCGAACTCATGGTTTAGACAGGTATGAACATTATATTCGTCGCCCGTCGGATTTTGGCAAACTTGAGCTGCAAGATTGGTTGAATCATAAGTCATTCCGAGTTTCCCCCAATTTATTAATTGATTCAAGCACCACACGTGAGTGGAATGAACCTGAGCTTTTTTATCAAAATACCGAGGATGAAACTTGGGTGAGACCTTGTGTAGGGCCAAAGCTAGAACCATCAATGATGATGCTCAGATATCACGATTCGAATATTGGTCAAATGCCTCAATTTTGTTATCCAATCTCAAGTCCGATAAATTTTAAACCAGTATTAAAATACATTTTGCAAGAACGTTCTGAACTGTCAGACGGCTTCCCTCAAAAGTATAACACACTAATAGGTAGTCTTTTTGACATTGATAAAAACCCAGAAACCTTAGATGATTCAGATATAGAAGCATTGGATGACATAGAAATGAGCAGTGACAGCGGTAATGTTAAAGA
ACCAAAAATTGAATTGCAGGCGCTGGAAGAAATCCAACAAAAGCATTTCA
GTTTAATAGTATCCAACAATGGAATCTTTCAAACAGGTAGCACTTCAATA
ACATACATACAGTCTGGCATATCTGGCAGCATAGCTATAAAACCCAACAA
CGTTGCAATTTTAATATTACTCACTCAACCAAGTGGTCACTTATTGTCTA
TTTTACCGCTTGATGACGGTAAAGAGACATATTTGCTACAATATTGGAAC
CTGGGACAAAAAGGTCAGTGGAACATAATCAAGCACCAAAACGAGAAGCA
GTTTGTTCTTATACATAAGGAACTAGGCATTTGCAAATTTTTTGAATTCC
ATTTACCATTTACTTTTCAATTAGTAAACAATTTAACATTGACCGATTCC
GTGATTATGAACGGATCCTTTTTCCCAACAAATTACACTGATTTAGATCC
CTATTTCATTATATTTATAACAGCCATAAGATATGAAAGGATAGTCTACT
TTGTCATAGAATGGAACAACAACGAAATAAAGAAAAAAGAGGTATATCAA
TTGACAGTATTTGATGGTGAGAAGACTAATATGACAATACCCATTGGACT
AAATGCATGTTTAGTCGAAACACCCCTAAAGTTCTCTTTAGTTTCTGCAA
ATCAAATTATGTCAGGAGAGACTGAATTCCACTCATTCCAATTGAAGGCT
CTCAAGGGAATCAAGTCATTTTTTCCAGCTCCTTTATTGTTATTAAAATT
ACAAGAACTACACCCACATACATTTAAAAAATTCCAATATTGTACCATAA
TATCCTCCTCGACAGGAAATATTTGTTTTTGCGTCACCGAACGATCGACA
ATAGTAAATGGTAATTTAAAATTTTACGAGCTAACTAGGTTCAAAGGATT
GAAATCCATTTCACCACTACCTTCAAATCCGATAAATTTAGACTCCAGAT
CCTCAAGTTATGTACTGGTGGTCATAAGTTTTAGTAGAACATTAGAGCTA
ACATTATCTCTAGAAGATTTGAGATGTTTAGATAAAAAAGACGTTATTAA
GCCTTTGAAAAATATCACCTTCAAGCACACAATTGATAGTTCCACAGAGG
AGAACTCTCAAATTTTAGCATTTACGTCTTCTAAATTTTATAACACACAC
ACAGGCTCTAACATCAATGACACGAGAAATTCTCAAGTTTGGCTTACCTC
ACCAAATGCAATAACTCAACCTTGCATTGATTATAAACTCAGGAAAACTC
ATCAACTTATCCACTTAAAGCAATTTCAAATTTTTAGACATCTTAGGATA
TGGAAATGTAAGAACCTTGATATTGCTCTGTTACAGAGACTTGGAATAAA
CCAGTCAAATACCGAAAGTTCGTTAATTTTTGCGACCGACGCTGTTTCTA
ACAACAGAATATTTTTATTAGATTTAACTATGACAACGACAATCGATAAT
GATGATCCCGTTCAAGGACTGATAAATATAGAAGATTTACTATGTGATAC
TGAAAACGAAACTATCCTTCTAAATTTTACGAAAAATAACTTGATTCAAG
TAACTAGGGATACGATATACATCGATCCCATTGGTGGGGACAAAGAGTTG
CGTAAGATTTCTCCAGGTTGGGAATTCGAAAACGTTACATACAATGATGG
TATTTTAATAGTTTGGAATGCTGGGCTTGGCTGTGTCTCTTATATTGAAA
ATATAGACGCTGTTGATGAGTCTGGCGCCTTAGTTTCAAACTTAAGCAGC
AGCAAAGGCATGAGCAAGTTCTTCAAGCAGTTAGGAACTGTCACGAGTGT
CAATTTTCAAATCAAAGAGTCTACAGATGATCCAACCAAGTATGACATTT
GGATCCTTTTACCAGACTGTGTCATTCGTACACCCTTTTCTGACTGGATT
AGTGATTCACTTGATTTTTCTGATGTGTATATTTTGAGTGTTCAGCAGGC
GTTAATAAACGGCCCTTATTTTTGCTCTCTCGATTATGAATCATATTTTG
AGGTGCACACTTTACAGAACAACTGCTTCAAAAAAGGATCCAGATGTACA
AGCAGAGTTAATTTTCAAGGGAAAGATATTAAATTTAGAAGCTTTGGTGT
GAATCAATGTTTGGCATTTAGTGCATTTGAAATTTTTGTCATCAATTTAA
CGCCAATTCACGACAGTAGAGAATTGGATTTTTACAAGCTAAAATTACCG
CACCTAGGTAATAACAATTCAATTCTTGAAGTCTGTCCGGACATAGAAAA
CAACCAATTATTCATACTCTACTCTGATGGCTTAAGAATCCTCGAACTAT
CATACCTAACATCAAATAATGGAAATTTCTTATTAAAATCTACAAGAAGC
AAAAATAAGAAATTTTTATATCTAGACAAAATAAATCGAATGCTGGTATT
GAATCAGGACTTGAGGGAATGGGAGTGTATTAGACTATCAGATGGTAAGG
CAGTTGGTTTAGATTCTCAACTTCTTAAGGATGATTCTGAAGAAATCCTA
GAAATAAAGGAATTACCAATAGCAACAGAGGACAATCCTTTAGAAAAGAA
AACTGTATTATTGATCTCTTTTACTAGCTCATTAAAACTAGTTTTATTAA
CTGCTGCAAAAAACAAAATTTCCAATCAAATAATAGATTCGTATAAACTT
GACAATTCAAGACTCCTCAATCATTTGGTCATTACTCCCAGAGGTGAAAT
ATTCTTTCTGGATTATAAAGTTATGGGCACCGATAACGAAATGTCCTTTA
ACAAGTTGAAAGTCACAAAACATTGCATTGACCAGGAGGAGAGAAATAAT
ACGACTTTGCGGCTCACTTTGGAAACCCGGTTTACATTTAAGAGTTGGAG
TACAGTTAAGACGTTTACTGTAGTAGGCGATAATATCATTGCTACCACAA
ATATGGGCGAAAAGCTCTACTTGATTAAGGATTTCTCTTCATCATCTGAC
GAATCGAGAAGGGTGTATCCCTTGGAAATGTATCCTGATTCAAAAGTTCA
AAAGATAATACCATTAAATGAATGCTGCTTTGTTGTTGCCGCTTACTGTG
GAAATAGGAACGATTTAGATTCAAGATTAATTTTTTACTCTTTACCCACC
ATCAAAGTTGGGCTTAATAACGAAACAGGCAGCCTACCAGATGAATATGG
GAATGGGAGAGTCGACGACATATTCGAGGTTGACTTTCCTGAAGGATTTC
AATTTGGCACTATGGCTTTGTATGATGTTTTACATGGTGAGAGGCACGTA
AATCGTTACAGCGAAGGCATACGGTCGGAGAATGACGAAGCAGAGGTTGC
CCTAAGGCAGCGTAGAAATTTACTACTCTTTTGGCGAAACCACTCTTCTA
CACCAAAACCTTCACTACGCCGAGCCGCCACTATAGTATATGAGGATCAT
GTATCATCCCGTTATTTTGAGGATATAAGTTCTATATTAGGAAGCACTGC
AATGAGAACTAAAAGACTATCTCCCTATAATGCGGTAGCATTGGACAAGC
CTATTCAAGATATTAGTTACGATCCCGCAGTACAAACTTTATATGTGCTA
ATGGCAGATCAAACAATTCACAAATTCGGCAAGGACAGGTTGCCTTGCCA
GGACGAATACGAACCAAGATGGAATTCTGGCTATTTGGTTTCAAGAAGGT
CAATAGTTAAATCTGACCTCATCTGTGAGGTTGGGTTATGGAACCTTAGC
GATAACTGCAAGAACACAGTACACCATCACCATCACCATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFPGRLERPHMLGLRTHG
LDRYEHYIRRPSDFGKLELQDWLNHKSFRVSPNLLIDSSTTREWNEPELF
YQNTEDETWVRPCVGPKLEPSMMMLRYHDSNIGQMPQFCYPISSPINFKP
VLKYILQERSELSDGFPQKYNTLIGSLFDIDKNPETLDDSDIEALDDIEM
SSDSGNVKEPKIELQALEEIQQKHFSLIVSNNGIFQTGSTSITYIQSGIS
GSIAIKPNNVAILILLTQPSGHLLSILPLDDGKETYLLQYWNLGQKGQWN
IIKHQNEKQFVLIHKELGICKFFEFHLPFTFQLVNNLTLTDSVIMNGSFF
PTNYTDLDPYFIIFITAIRYERIVYFVIEWNNNEIKKKEVYQLTVFDGEK
TNMTIPIGLNACLVETPLKFSLVSANQIMSGETEFHSFQLKALKGIKSFF
PAPLLLLKLQELHPHTFKKFQYCTIISSSTGNICFCVTERSTIVNGNLKF
YELTRFKGLKSISPLPSNPINLDSRSSSYVLVVISFSRTLELTLSLEDLR
CLDKKDVIKPLKNITFKHTIDSSTEENSQILAFTSSKFYNTHTGSNINDT
RNSQVWLTSPNAITQPCIDYKLRKTHQLIHLKQFQIFRHLRIWKCKNLDI
ALLQRLGINQSNTESSLIFATDAVSNNRIFLLDLTMTTTIDNDDPVQGLI
NIEDLLCDTENETILLNFTKNNLIQVTRDTIYIDPIGGDKELRKISPGWE
FENVTYNDGILIVWNAGLGCVSYIENIDAVDESGALVSNLSSSKGMSKFF
KQLGTVTSVNFQIKESTDDPTKYDIWILLPDCVIRTPFSDWISDSLDFSD
VYILSVQQALINGPYFCSLDYESYFEVHTLQNNCFKKGSRCTSRVNFQGK
DIKFRSFGVNQCLAFSAFEIFVINLTPIHDSRELDFYKLKLPHLGNNNSI
LEVCPDIENNQLFILYSDGLRILELSYLTSNNGNFLLKSTRSKNKKFLYL
DKINRMLVLNQDLREWECIRLSDGKAVGLDSQLLKDDSEEILEIKELPIA
TEDNPLEKKTVLLISFTSSLKLVLLTAAKNKISNQIIDSYKLDNSRLLNH
LVITPRGEIFFLDYKVMGTDNEMSFNKLKVTKHCIDQEERNNTTLRLTLE
TRFTFKSWSTVKTFTVVGDNIIATTNMGEKLYLIKDFSSSSDESRRVYPL
EMYPDSKVQKIIPLNECCFVVAAYCGNRNDLDSRLIFYSLPTIKVGLNNE
TGSLPDEYGNGRVDDIFEVDFPEGFQFGTMALYDVLHGERHVNRYSEGIR
SENDEAEVALRQRRNLLLFWRNHSSTPKPSLRRAATIVYEDHVSSRYFED
ISSILGSTAMRTKRLSPYNAVALDKPIQDISYDPAVQTLYVLMADQTIHK
FGKDRLPCQDEYEPRWNSGYLVSRRSIVKSDLICEVGLWNLSDNCKNTVH
HHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11350
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 190198 Daltons