cDNA Clone Claspin

• Plasmid: pFastBacHTb Claspin
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-Claspin
• Genbank: NM_022111
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGACAGGCGAGGTGGGTTCTGAGGTTCACCTAGAAATCAATGACCCAAACGTCATTTCACAAGAGGAAGCAGATAGTCCTTCAGATAGTGGACAGGGCAGCTATGAAACAATTGGACCCTTGAGTGAAGGAGATTCAGATGAAGAGATATTTGTAAGTAAGAAGTTGAAAAACAGGAAGGTTCTACAAGACAGTGATTCCGAAACAGAGGACACAAATGCCTCTCCAGAGAAAACTACCTATGACAGTGCCGAGGAGGAAAATAAAGAGAATTTATATGCTGGGAAAAATACAAAAATC
AAAAGGATTTACAAAACTGTGGCAGACAGTGATGAAAGTTACATGGAAAA
GTCTTTGTATCAGGAAAATCTTGAAGCGCAAGTGAAACCTTGCTTAGAGC
TGAGTCTTCAGTCTGGAAACTCTACAGACTTTACCACTGACAGAAAGAGT
TCCAAAAAGCACATACATGATAAAGAAGGAACTGCAGGAAAAGCAAAAGT
AAAATCAAAAAGAAGACTTGAGAAAGAGGAGAGAAAAATGGAAAAAATTA
GACAGCTAAAAAAGAAGGAAACAAAAAACCAGGAAGATGATGTAGAACAG
CCATTTAATGACAGTGGCTGTCTTCTTGTGGATAAAGACCTTTTTGAAAC
TGGGTTGGAGGATGAAAATAACTCTCCATTGGAAGATGAAGAGTCATTAG
AATCAATAAGAGCAGCTGTAAAAAACAAAGTAAAAAAGCACAAGAAAAAA
GAACCATCTTTGGAGAGTGGGGTCCATTCATTTGAGGAAGGAAGTGAGTT
ATCAAAAGGAACCACGAGGAAGGAAAGAAAGGCAGCCAGATTAAGTAAAG
AAGCATTAAAACAACTGCATAGTGAGACTCAGCGCCTTATTCGAGAGTCT
GCACTGAACCTTCCATATCATATGCCTGAGAATAAAACCATTCATGATTT
CTTCAAACGTAAACCCCGGCCCACTTGCCACGGAAATGCCATGGCACTAT
TGAAGTCATCTAAATATCAGTCAAGCCATCACAAAGAAATCATAGACACT
GCAAATACTACTGAAATGAACAGTGATCACCATAGTAAAGGTTCTGAGCA
GACAACAGGTGCAGAAAATGAAGTGGAAACTAATGCACTCCCTGTAGTTT
CAAAGGAAACCCAGATCATTACTGGATCAGATGAGTCTTGCAGGAAGGAT
TTGGTAAAAAATGAAGAGCTAGAAATTCAGGAGAAACAGAAGCAGAGTGA
CATTAGACCTTCACCTGGGGACAGCTCAGTGTTGCAACAGGAATCCAACT
TCCTCGGGAACAATCACAGTGAGGAATGTCAGGTTGGAGGGCTTGTAGCA
TTTGAACCTCATGCCCTGGAGGGTGAAGGCCCCCAAAATCCAGAAGAAAC
AGATGAGAAAGTGGAAGAGCCTGAGCAGCAAAATAAATCATCAGCAGTTG
GGCCACCTGAAAAAGTGAGACGGTTTACTCTGGATAGACTTAAGCAACTG
GGAGTAGATGTTTCCATTAAACCACGGCTAGGTGCTGATGAAGATTCCTT
TGTGATACTTGAACCTGAAACCAACAGAGAACTGGAAGCCTTGAAGCAGC
GTTTCTGGAAGCATGCTAATCCAGCAGCCAAACCCAGGGCTGGTCAGACA
GTGAATGTGAACGTCATAGTGAAAGACATGGGCACTGATGGAAAGGAAGA
GCTAAAAGCAGATGTGGTACCTGTGACTTTAGCACCTAAGAAGTTGGATG
GGGCAAGCCACACAAAACCAGGTGAAAAGCTTCAGGTGTTAAAAGCTAAA
CTGCAAGAAGCAATGAAACTCCGAAGGTTTGAGGAGCGCCAGAAGCGCCA
AGCACTGTTTAAATTAGATAATGAAGATGGGTTTGAGGAAGAGGAGGAGG
AAGAGGAAGAAATGACAGATGAGTCTGAGGAAGATGGAGAAGAGAAGGTA
GAGAAAGAAGAGAAAGAGGAAGAACTAGAGGAAGAGGAGGAGAAAGAAGA
GGAGGAGGAAGAAGAAGGAAATCAGGAGACTGCAGAATTCCTTCTTAGTA
GTGAAGAAATAGAAACAAAAGATGAAAAAGAAATGGATAAAGAAAATAAT
GATGGCAGTAGTGAAATTGGCAAGGCAGTTGGCTTCCTCTCTGTTCCCAA
GTCTCTCTCATCAGATTCTACTTTACTTCTGTTTAAGGACAGCTCTTCCA
AGATGGGTTACTTTCCTACTGAAGAAAAATCAGAAACAGATGAAAACTCA
GGCAAGCAGCCTAGCAAACTGGATGAGGATGATTCATGTTCATTGCTAAC
AAAGGAGAGCAGCCACAATAGCAGCTTTGAGCTGATTGGCTCCACGATTC
CATCCTATCAGCCTTGCAACAGACAAACAGGCCGTGGGACCAGTTTTTTC
CCTACAGCAGGAGGATTCAGATCTCCTTCCCCTGGGCTATTTCGAGCCAG
TTTGGTCAGCTCAGCTTCTAAGAGTTCAGGGAAACTGTCTGAGCCTTCAC
TTCCCATAGAGGATTCCCAGGATCTGTATAACGCCTCCCCAGAGCCTAAG
ACACTTTTCCTAGGAGCAGGAGACTTCCAGTTCTGTTTAGAAGATGACAC
TCAGAGCCAACTGTTGGATGCAGATGGGTTCTTAAATGTTAGAAACCACA
GGAATCAGTACCAAGCTTTGAAGCCTCGATTGCCATTGGCCAGTATGGAT
GAGAATGCCATGGATGCCAACATGGATGAGCTGTTGGATTTGTGTACTGG
AAAGTTCACATCTCAGGCTGAAAAACATCTACCCAGGAAGAGTGACAAGA
AAGAGAACATGGAGGAACTTCTGAACCTTTGTTCAGGAAAATTCACTTCT
CAGGATGCCTCCACTCCAGCCTCATCAGAGTTAAATAAACAGGAGAAGGA
GAGCAGCATGGGTGATCCAATGGAAGAAGCACTTGCTCTTTGCTCAGGCT
CTTTTCCCACAGACAAGGAAGAGGAAGACGAGGAGGAGGAATTTGGAGAC
TTTCGGCTTGTTTCAAATGATAATGAGTTTGATAGTGATGAGGATGAACA
CAGTGACTCTGGTAATGATCTGGCACTGGAAGACCATGAAGATGATGATG
AAGAAGAACTCCTGAAGCGATCTGAGAAGTTGAAAAGGCAAATGAGGTTG
AGGAAATACCTGGAGGATGAGGCAGAGGTGTCAGGAAGTGATGTGGGAAG
CGAAGATGAGTATGATGGGGAAGAAATTGATGAATATGAAGAGGACGTAA
TTGATGAAGTACTTCCTTCTGATGAGGAACTGCAGAGTCAAATCAAGAAA
ATACACATGAAAACTATGTTGGATGATGATAAGCGACAGCTACGTTTATA
CCAAGAGAGGTACCTTGCTGATGGGGATCTGCACAGCGATGGTCCTGGGC
GAATGAGGAAGTTTCGATGGAAAAACATAGATGATGCTTCCCAGATGGAC
TTGTTCCACAGAGACTCTGATGATGATCAGACTGAAGAACAGCTTGATGA
GTCAGAAGCCAGGTGGAGGAAGGAGCGAATTGAACGAGAGCAGTGGCTTC
GGGACATGGCACAGCAGGGGAAAATTACAGCTGAAGAAGAAGAAGAAATT
GGGGAGGACAGTCAGTTTATGATACTGGCCAAGAAAGTTACAGCCAAAGC
ACTGCAGAAGAATGCCAGTCGCCCTATGGTTATTCAGGAATCAAAGTCTT
TGCTCAGAAATCCTTTTGAAGCCATCAGACCAGGAAGTGCTCAACAGGTG
AAGACAGGCTCACTGCTAAACCAGCCCAAAGCTGTGCTTCAGAAACTGGC
TGCTCTCTCTGACCATAACCCCAGTGCTCCTCGAAATTCAAGAAACTTTG
TCTTTCATACACTTTCTCCTGTCAAGGCTGAGGCGGCAAAGGAATCGTCT
AAGTCTCAGGTAAAGAAAAGGGGTCCATCTTTCATGACTTCTCCTTCACC
TAAGCACCTCAAAACAGATGATAGCACTTCAGGATTGACGCGAAGCATCT
TCAAATATTTGGAGAGCTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMTGEVGSEVHLEINDPNVISQE
EADSPSDSGQGSYETIGPLSEGDSDEEIFVSKKLKNRKVLQDSDSETEDT
NASPEKTTYDSAEEENKENLYAGKNTKIKRIYKTVADSDESYMEKSLYQE
NLEAQVKPCLELSLQSGNSTDFTTDRKSSKKHIHDKEGTAGKAKVKSKRR
LEKEERKMEKIRQLKKKETKNQEDDVEQPFNDSGCLLVDKDLFETGLEDE
NNSPLEDEESLESIRAAVKNKVKKHKKKEPSLESGVHSFEEGSELSKGTT
RKERKAARLSKEALKQLHSETQRLIRESALNLPYHMPENKTIHDFFKRKP
RPTCHGNAMALLKSSKYQSSHHKEIIDTANTTEMNSDHHSKGSEQTTGAE
NEVETNALPVVSKETQIITGSDESCRKDLVKNEELEIQEKQKQSDIRPSP
GDSSVLQQESNFLGNNHSEECQVGGLVAFEPHALEGEGPQNPEETDEKVE
EPEQQNKSSAVGPPEKVRRFTLDRLKQLGVDVSIKPRLGADEDSFVILEP
ETNRELEALKQRFWKHANPAAKPRAGQTVNVNVIVKDMGTDGKEELKADV
VPVTLAPKKLDGASHTKPGEKLQVLKAKLQEAMKLRRFEERQKRQALFKL
DNEDGFEEEEEEEEEMTDESEEDGEEKVEKEEKEEELEEEEEKEEEEEEE
GNQETAEFLLSSEEIETKDEKEMDKENNDGSSEIGKAVGFLSVPKSLSSD
STLLLFKDSSSKMGYFPTEEKSETDENSGKQPSKLDEDDSCSLLTKESSH
NSSFELIGSTIPSYQPCNRQTGRGTSFFPTAGGFRSPSPGLFRASLVSSA
SKSSGKLSEPSLPIEDSQDLYNASPEPKTLFLGAGDFQFCLEDDTQSQLL
DADGFLNVRNHRNQYQALKPRLPLASMDENAMDANMDELLDLCTGKFTSQ
AEKHLPRKSDKKENMEELLNLCSGKFTSQDASTPASSELNKQEKESSMGD
PMEEALALCSGSFPTDKEEEDEEEEFGDFRLVSNDNEFDSDEDEHSDSGN
DLALEDHEDDDEEELLKRSEKLKRQMRLRKYLEDEAEVSGSDVGSEDEYD
GEEIDEYEEDVIDEVLPSDEELQSQIKKIHMKTMLDDDKRQLRLYQERYL
ADGDLHSDGPGRMRKFRWKNIDDASQMDLFHRDSDDDQTEEQLDESEARW
RKERIEREQWLRDMAQQGKITAEEEEEIGEDSQFMILAKKVTAKALQKNA
SRPMVIQESKSLLRNPFEAIRPGSAQQVKTGSLLNQPKAVLQKLAALSDH
NPSAPRNSRNFVFHTLSPVKAEAAKESSKSQVKKRGPSFMTSPSPKHLKT
DDSTSGLTRSIFKYLES*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU11286
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; Sequence difference compared with NM_022111 CDS:; a1752g (silent) - originates from EST 7961377 used for amplification.; ; Estimated MW of expressed protein : 154461 Daltons