cDNA Clone ATR

• Plasmid: pCMV-FLAG3 ATR
• Parent Plasmid: pCMV5 FLAG3
• Expressed: FLAG-ATR
• Genbank: Q13535
• Tag: FLAG
• Species: Human

Sequence of Insert

gcggccgcatgggggaacatggcctggagctggcttccatgatccccgccctgcgggagctgggcagtgccacaccagaggaatataatacagttgtacagaagccaagacaaattctgtgtcaattcattgaccggatacttacagatgtaaatgttgttgctgtagaacttgtaaagaaaactgactctcagccaacctccgtgatgttgcttgatttcatccagcatatcatgaaatcctccccacttatgtttgtaaatgtgagtggaagccatgaggccaaaggcagttgtattgaattcagtaattggatcataacgagacttctgcggattgcagcaactccctcctgtcatttgttacacaagaaaatctgtgaagtcatctgttcattattatttcttt
ttaaaagcaagagtcctgctatttttggggtactcacaaaagaattatta
caactttttgaagacttggtttacctccatagaagaaatgtgatgggtca
tgctgtggaatggccagtggtcatgagccgatttttaagtcaattagatg
aacacatgggatatttacaatcagctcctttgcagttgatgagtatgcaa
aatttagaatttattgaagtcactttattaatggttcttactcgtattat
tgcaattgtgttttttagaaggcaagaactcttactttggcagataggtt
gtgttctgctagagtatggtagtccaaaaattaaatccctagcaattagc
tttttaacagaactttttcagcttggaggactaccagcacaaccagctag
cacttttttcagctcatttttggaattattaaaacaccttgtagaaatgg
atactgaccaattgaaactctatgaagagccattatcaaagctgataaag
acactatttccctttgaagcagaagcttatagaaatattgaacctgtcta
tttaaatatgctgctggaaaaactctgtgtcatgtttgaagacggtgtgc
tcatgcggcttaagtctgatttgctaaaagcagctttgtgccatttactg
cagtatttccttaaatttgtgccagctgggtatgaatctgctttacaagt
caggaaggtctatgtgagaaatatttgtaaagctcttttggatgtgcttg
gaattgaggtagatgcagagtacttgttgggcccactttatgcagctttg
aaaatggaaagtatggaaatcattgaggagattcaatgccaaactcaaca
ggaaaacctcagcagtaatagtgatggaatatcacccaaaaggcgtcgtc
tcagctcgtctctaaacccttctaaaagagcaccaaaacagactgaggaa
attaaacatgtggacatgaaccaaaagagcatattatggagtgcactgaa
acagaaagctgaatcccttcagatttcccttgaatacagtggcctaaaga
atcctgttattgagatgttagaaggaattgctgttgtcttacaactgact
gctctgtgtactgttcattgttctcatcaaaacatgaactgccgtacttt
caaggactgtcaacataaatccaagaagaaaccttctgtagtgataactt
ggatgtcattggatttttacacaaaagtgcttaagagctgtagaagtttg
ttagaatctgttcagaaactggacctggaggcaaccattgataaggtggt
gaaaatttatgatgctttgatttatatgcaagtaaacagttcatttgaag
atcatatcctggaagatttatgtggtatgctctcacttccatggatttat
tcccattctgatgatggctgtttaaagttgaccacatttgccgctaatct
tctaacattaagctgtaggatttcagatagctattcaccacaggcacaat
cacgatgtgtgtttcttctgactctgtttccaagaagaatattccttgag
tggagaacagcagtttacaactgggccctgcagagctcccatgaagtaat
ccgggctagttgtgttagtggattttttatcttattgcagcagcagaatt
cttgtaacagagttcccaagattcttatagataaagtcaaagatgattct
gacattgtcaagaaagaatttgcttctatacttggtcaacttgtctgtac
tcttcacggcatgttttatctgacaagttctttaacagaacctttctctg
aacacggacatgtggacctcttctgtaggaacttgaaagccacttctcaa
catgaatgttcatcttctcaactaaaagcttctgtctgcaagccattcct
tttcctactgaaaaaaaaaatacctagtccagtaaaacttgctttcatag
ataatctacatcatctttgtaagcatcttgattttagagaagatgaaaca
gatgtaaaagcagttcttggaactttattaaatttaatggaagatccaga
caaagatgttagagtggcttttagtggaaatatcaagcacatattggaat
ccttggactctgaagatggatttataaaggagctttttgtcttaagaatg
aaggaagcatatacacatgcccaaatatcaagaaataatgagctgaagga
taccttgattcttacaacaggggatattggaagggccgcaaaaggagatt
tggtaccatttgcactcttacacttattgcattgtttgttatccaagtca
gcatctgtctctggagcagcatacacagaaattagagctctggttgcagc
taaaagtgttaaactgcaaagttttttcagccagtataagaaacccatct
gtcagtttttggtagaatcccttcactctagtcagatgacagcacttccg
aatactccatgccagaatgctgacgtgcgaaaacaagatgtggctcacca
gagagaaatggctttaaatacgttgtctgaaattgccaacgttttcgact
ttcctgatcttaatcgttttcttactaggacattacaagttctactacct
gatcttgctgccaaagcaagccctgcagcttctgctctcattcgaacttt
aggaaaacaattaaatgtcaatcgtagagagattttaataaacaacttca
aatatattttttctcatttggtctgttcttgttccaaagatgaattagaa
cgtgcccttcattatctgaagaatgaaacagaaattgaactggggagcct
gttgagacaagatttccaaggattgcataatgaattattgctgcgtattg
gagaacactatcaacaggtttttaatggtttgtcaatacttgcctcattt
gcatccagtgatgatccatatcagggcccgagagatatcatatcacctga
actgatggctgattatttacaacccaaattgttgggcattttggcttttt
ttaacatgcagttactgagctctagtgttggcattgaagataagaaaatg
gccttgaacagtttgatgtctttgatgaagttaatgggacccaaacatgt
cagttctgtgagggtgaagatgatgaccacactgagaactggccttcgat
tcaaggatgattttcctgaattgtgttgcagagcttgggactgctttgtt
cgctgcctggatcatgcttgtctgggctcccttctcagtcatgtaatagt
agctttgttacctcttatacacatccagcctaaagaaactgcagctatct
tccactacctcataattgaaaacagggatgctgtgcaagattttcttcat
gaaatatattttttacctgatcatccagaattaaaaaagataaaagccgt
tctccaggaatacagaaaggagacctctgagagcactgatcttcagacaa
ctcttcagctctctatgaaggccattcaacatgaaaatgtcgatgttcgt
attcatgctcttacaagcttgaaggaaaccttgtataaaaatcaggaaaa
actgataaagtatgcaacagacagtgaaacagtagaacctattatctcac
agttggtgacagtgcttttgaaaggttgccaagatgcaaactctcaagct
cggttgctctgtggggaatgtttaggggaattgggggcgatagatccagg
tcgattagatttctcaacaactgaaactcaaggaaaagattttacatttg
tgactggagtagaagattcaagctttgcctatggattattgatggagcta
acaagagcttaccttgcgtacgctgataatagccgagctcaagattcagc
tgcctatgccattcaggagttgctttctatttatgactgtagagagatgg
agaccaacggcccaggtcaccaattgtggaggagatttcctgagcatgtt
cgggaaatactagaacctcatctaaataccagatacaagagttctcagaa
gtcaaccgattggtctggagtaaagaagccaatttacttaagtaaattgg
gtagtaactttgcagaatggtcagcatcttgggcaggttatcttattaca
aaggttcgacatgatcttgccagtaaaattttcacctgctgtagcattat
gatgaagcatgatttcaaagtgaccatctatcttcttccacatattctgg
tgtatgtcttactgggttgtaatcaagaagatcagcaggaggtttatgca
gaaattatggcagttctaaagcatgacgatcagcataccataaataccca
agacattgcatctgatctgtgtcaactcagtacacagactgtgttctcca
tgcttgaccatctcacacagtgggcaaggcacaaatttcaggcactgaaa
gctgagaaatgtccacacagcaaatcaaacagaaataaggtagactcaat
ggtatctactgtggattatgaagactatcagagtgtaacccgttttctag
acctcataccccaggatactctggcagtagcttcctttcgctccaaagca
tacacacgagctgtaatgcactttgaatcatttattacagaaaagaagca
aaatattcaggaacatcttggatttttacagaaattgtatgctgctatgc
atgaacctgatggagtggccggagtcagtgcaattagaaaggcagaacca
tctctaaaagaacagatccttgaacatgaaagccttggcttgctgaggga
tgccactgcttgttatgacagggctattcagctagaaccagaccagatca
ttcattatcatggtgtagtaaagtccatgttaggtcttggtcagctgtct
actgttatcactcaggtgaatggagtgcatgctaacaggtccgagtggac
agatgaattaaacacgtacagagtggaagcagcttggaaattgtcacagt
gggatttggtggaaaactatttggcagcagatggaaaatctacaacatgg
agtgtcagactgggacagctattattatcagccaaaaaaagagatatcac
agctttttatgactcactgaaactagtgagagcagaacaaattgtacctc
tttcagctgcaagctttgaaagaggctcctaccaacgaggatatgaatat
attgtgagattgcacatgttatgtgagttggagcatagcatcaaaccact
tttccagcattctccaggtgacagttctcaagaagattctctaaactggg
tagctcgactagaaatgacccagaattcctacagagccaaggagcctatc
ctggctctccggagggctttactaagcctcaacaaaagaccagattacaa
tgaaatggttggagaatgctggctgcagagtgccagggtagctagaaagg
ctggtcaccaccagacagcctacaatgctctccttaatgcaggggaatca
cgactcgctgaactgtacgtggaaagggcaaagtggctctggtccaaggg
tgatgttcaccaggcactaattgttcttcaaaaaggtgttgaattatgtt
ttcctgaaaatgaaaccccacctgagggtaagaacatgttaatccatggt
cgagctatgctactagtgggccgatttatggaagaaacagctaactttga
aagcaatgcaattatgaaaaaatataaggatgtgaccgcgtgcctgccag
aatgggaggatgggcatttttaccttgccaagtactatgacaaattgatg
cccatggtcacagacaacaaaatggaaaagcaaggtgatctcatccggta
tatagttcttcattttggcagatctctacaatatggaaatcagttcatat
atcagtcaatgccacgaatgttaactctatggcttgattatggtacaaag
gcatatgaatgggaaaaagctggccgctccgatcgtgtacaaatgaggaa
tgatttgggtaaaataaacaaggttatcacagagcatacaaactatttag
ctccatatcaatttttgactgctttttcacaattgatctctcgaatttgt
cattctcacgatgaagtttttgttgtcttgatggaaataatagccaaagt
atttctagcctatcctcaacaagcaatgtggatgatgacagctgtgtcaa
agtcatcttatcccatgcgtgtgaacagatgcaaggaaatcctcaataaa
gctattcatatgaaaaaatccttagagaagtttgttggagatgcaactcg
cctaacagataagcttctagaattgtgcaataaaccggttgatggaagta
gttccacattaagcatgagcactcattttaaaatgcttaaaaagctggta
gaagaagcaacatttagtgaaatcctcattcctctacaatcagtcatgat
acctacacttccatcaattctgggtacccatgctaaccatgctagccatg
aaccatttcctggacattgggcctatattgcagggtttgatgatatggtg
gaaattcttgcttctcttcagaaaccaaagaagatttctttaaaaggctc
agatggaaagttctacatcatgatgtgtaagccaaaagatgacctgagaa
aggattgtagactaatggaattcaattccttgattaataagtgcttaaga
aaagatgcagagtctcgtagaagagaacttcatattcgaacatatgcagt
tattccactaaatgatgaatgtgggattattgaatgggtgaacaacactg
ctggtttgagacctattctgaccaaactatataaagaaaagggagtgtat
atgacaggaaaagaacttcgccagtgtatgctaccaaagtcagcagcttt
atctgaaaaactcaaagtattccgagaatttctcctgcccaggcatcctc
ctatttttcatgagtggtttctgagaacattccctgatcctacatcatgg
tacagtagtagatcagcttactgccgttccactgcagtaatgtcaatggt
tggttatattctggggcttggagaccgtcatggtgaaaatattctctttg
attctttgactggtgaatgcgtacatgtagatttcaattgtcttttcaat
aagggagaaacctttgaagttccagaaattgtgccatttcgcctgactca
taatatggttaatggaatgggtcctatgggaacagagggtctttttcgaa
gagcatgtgaagttacaatgaggctgatgcgtgatcagcgagagccttta
atgagtgtcttaaagacttttctacatgatcctcttgtggaatggagtaa
accagtgaaagggcattccaaagcgccactgaatgaaactggagaagttg
tcaatgaaaaggccaagacccatgttcttgacattgagcagcgactacaa
ggtgtaatcaagactcgaaatagagtgacaggactgccgttatctattga
aggacatgtgcattaccttatacaggaagctactgatgaaaacttactat
gccagatgtatcttggttggactccatatatgtgaAATTATGTAAAAGAATATGTTAATAATCTAAAAGTAATGCATTTGGTATGAATCTGTGGTTGTATCTGTTCAATTCTAAAGTACAACATAAATTTACGTT
CTCAGCAACTGTTATTTCTCTCTGATCATTAATTATATGTAAAATAATATACATTCACTCGTGCCGAATTCGGCACGAGAATTGGCACTGATGAGGATTT
AGCGCACTCTTCTATCAGGTTTGGAATTGGCCGCTTCACTACAGAGGAGGAAGTGGACTACACAGTGGAGAAATGCATTCAGCATGTGAAGCGTCTTCGA
GAAATGAGCCCTCTCTGGGAGATGGTTCAGGATGGCATTGACCTCAAGAGCATCAAGTGGACCCAACACTAGAAGAATAGGGCCCTGACTTTGTGCTGGT
CTGGCCCTCGTGCCGCTCGTGCCGAATTCCTGCAGCCCGGGGGATCCCCTAGG
gcggccgc

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDSSGRMGEHGLELASMIPALRELGSATPEEYNT
VVQKPRQILCQFIDRILTDVNVVAVELVKKTDSQPTSVMLLDFIQHIMKS
SPLMFVNVSGSHEAKGSCIEFSNWIITRLLRIAATPSCHLLHKKICEVIC
SLLFLFKSKSPAIFGVLTKELLQLFEDLVYLHRRNVMGHAVEWPVVMSRF
LSQLDEHMGYLQSAPLQLMSMQNLEFIEVTLLMVLTRIIAIVFFRRQELL
LWQIGCVLLEYGSPKIKSLAISFLTELFQLGGLPAQPASTFFSSFLELLK
HLVEMDTDQLKLYEEPLSKLIKTLFPFEAEAYRNIEPVYLNMLLEKLCVM
FEDGVLMRLKSDLLKAALCHLLQYFLKFVPAGYESALQVRKVYVRNICKA
LLDVLGIEVDAEYLLGPLYAALKMESMEIIEEIQCQTQQENLSSNSDGIS
PKRRRLSSSLNPSKRAPKQTEEIKHVDMNQKSILWSALKQKAESLQISLE
YSGLKNPVIEMLEGIAVVLQLTALCTVHCSHQNMNCRTFKDCQHKSKKKP
SVVITWMSLDFYTKVLKSCRSLLESVQKLDLEATIDKVVKIYDALIYMQV
NSSFEDHILEDLCGMLSLPWIYSHSDDGCLKLTTFAANLLTLSCRISDSY
SPQAQSRCVFLLTLFPRRIFLEWRTAVYNWALQSSHEVIRASCVSGFFIL
LQQQNSCNRVPKILIDKVKDDSDIVKKEFASILGQLVCTLHGMFYLTSSL
TEPFSEHGHVDLFCRNLKATSQHECSSSQLKASVCKPFLFLLKKKIPSPV
KLAFIDNLHHLCKHLDFREDETDVKAVLGTLLNLMEDPDKDVRVAFSGNI
KHILESLDSEDGFIKELFVLRMKEAYTHAQISRNNELKDTLILTTGDIGR
AAKGDLVPFALLHLLHCLLSKSASVSGAAYTEIRALVAAKSVKLQSFFSQ
YKKPICQFLVESLHSSQMTALPNTPCQNADVRKQDVAHQREMALNTLSEI
ANVFDFPDLNRFLTRTLQVLLPDLAAKASPAASALIRTLGKQLNVNRREI
LINNFKYIFSHLVCSCSKDELERALHYLKNETEIELGSLLRQDFQGLHNE
LLLRIGEHYQQVFNGLSILASFASSDDPYQGPRDIISPELMADYLQPKLL
GILAFFNMQLLSSSVGIEDKKMALNSLMSLMKLMGPKHVSSVRVKMMTTL
RTGLRFKDDFPELCCRAWDCFVRCLDHACLGSLLSHVIVALLPLIHIQPK
ETAAIFHYLIIENRDAVQDFLHEIYFLPDHPELKKIKAVLQEYRKETSES
TDLQTTLQLSMKAIQHENVDVRIHALTSLKETLYKNQEKLIKYATDSETV
EPIISQLVTVLLKGCQDANSQARLLCGECLGELGAIDPGRLDFSTTETQG
KDFTFVTGVEDSSFAYGLLMELTRAYLAYADNSRAQDSAAYAIQELLSIY
DCREMETNGPGHQLWRRFPEHVREILEPHLNTRYKSSQKSTDWSGVKKPI
YLSKLGSNFAEWSASWAGYLITKVRHDLASKIFTCCSIMMKHDFKVTIYL
LPHILVYVLLGCNQEDQQEVYAEIMAVLKHDDQHTINTQDIASDLCQLST
QTVFSMLDHLTQWARHKFQALKAEKCPHSKSNRNKVDSMVSTVDYEDYQS
VTRFLDLIPQDTLAVASFRSKAYTRAVMHFESFITEKKQNIQEHLGFLQK
LYAAMHEPDGVAGVSAIRKAEPSLKEQILEHESLGLLRDATACYDRAIQL
EPDQIIHYHGVVKSMLGLGQLSTVITQVNGVHANRSEWTDELNTYRVEAA
WKLSQWDLVENYLAADGKSTTWSVRLGQLLLSAKKRDITAFYDSLKLVRA
EQIVPLSAASFERGSYQRGYEYIVRLHMLCELEHSIKPLFQHSPGDSSQE
DSLNWVARLEMTQNSYRAKEPILALRRALLSLNKRPDYNEMVGECWLQSA
RVARKAGHHQTAYNALLNAGESRLAELYVERAKWLWSKGDVHQALIVLQK
GVELCFPENETPPEGKNMLIHGRAMLLVGRFMEETANFESNAIMKKYKDV
TACLPEWEDGHFYLAKYYDKLMPMVTDNKMEKQGDLIRYIVLHFGRSLQY
GNQFIYQSMPRMLTLWLDYGTKAYEWEKAGRSDRVQMRNDLGKINKVITE
HTNYLAPYQFLTAFSQLISRICHSHDEVFVVLMEIIAKVFLAYPQQAMWM
MTAVSKSSYPMRVNRCKEILNKAIHMKKSLEKFVGDATRLTDKLLELCNK
PVDGSSSTLSMSTHFKMLKKLVEEATFSEILIPLQSVMIPTLPSILGTHA
NHASHEPFPGHWAYIAGFDDMVEILASLQKPKKISLKGSDGKFYIMMCKP
KDDLRKDCRLMEFNSLINKCLRKDAESRRRELHIRTYAVIPLNDECGIIE
WVNNTAGLRPILTKLYKEKGVYMTGKELRQCMLPKSAALSEKLKVFREFL
LPRHPPIFHEWFLRTFPDPTSWYSSRSAYCRSTAVMSMVGYILGLGDRHG
ENILFDSLTGECVHVDFNCLFNKGETFEVPEIVPFRLTHNMVNGMGPMGT
EGLFRRACEVTMRLMRDQREPLMSVLKTFLHDPLVEWSKPVKGHSKAPLN
ETGEVVNEKAKTHVLDIEQRLQGVIKTRNRVTGLPLSIEGHVHYLIQEAT
DENLLCQMYLGWTPYM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5947
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 303868 Daltons; (insert contains extra sequence (approx 460 bp) between stop codon of ATR and 3' NotI site-derives from source clone)