cDNA Clone BRK

• Synonyms: PTK6
• Plasmid: pFastBacHTb BRK
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-BRK
• Genbank: NM_005975
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGTGTCCCGGGACCAGGCTCACCTGGGCCCCAAGTATGTGGGCCTCTGGGACTTCAAGTCCCGGACGGACGAGGAGCTGAGCTTCCGCGCGGGGGACGTCTTCCACGTGGCCAGGAAGGAGGAGCAGTGGTGGTGGGCCACGCTGCTGGACGAGGCGGGTGGGGCCGTGGCCCAGGGCTATGTGCCCCACAACTACCTGGCCGAGAGGGAGACGGTGGAGTCGGAACCGTGGTTCTTTGGCTGCATCTCCCGCTCGGAAGCTGTGCGTCGGCTGCAGGCCGAGGGCAACGCCACGGGC
GCCTTCCTGATCAGGGTCAGCGAGAAGCCGAGTGCCGACTACGTCCTGTC
GGTGCGGGACACGCAGGCTGTGCGGCACTACAAGATCTGGCGGCGTGCCG
GGGGCCGGCTGCACCTGAACGAGGCGGTGTCCTTCCTCAGCCTGCCCGAG
CTTGTGAACTACCACAGGGCCCAGAGCCTGTCCCACGGCCTGCGGCTGGC
CGCGCCCTGCCGGAAGCACGAGCCTGAGCCCCTGCCCCATTGGGATGACT
GGGAGAGGCCGAGGGAGGAGTTCACGCTCTGCAGGAAGCTGGGGTCCGGC
TACTTTGGGGAGGTCTTCGAGGGGCTCTGGAAAGACCGGGTCCAGGTGGC
CATTAAGGTGATTTCTCGAGACAACCTCCTGCACCAGCAGATGCTGCAGT
CGGAGATCCAGGCCATGAAGAAGCTGCGGCACAAACACATCCTGGCGCTG
TACGCCGTGGTGTCCGTGGGGGACCCCGTGTACATCATCACGGAGCTCAT
GGCCAAGGGCAGCCTGCTGGAGCTGCTCCGCGACTCTGATGAGAAAGTCC
TGCCCGTTTCGGAGCTGCTGGACATCGCCTGGCAGGTGGCTGAGGGCATG
TGTTACCTGGAGTCGCAGAATTACATCCACCGGGACCTGGCCGCCAGGAA
CATCCTCGTCGGGGAAAACACCCTCTGCAAAGTTGGGGACTTCGGGTTAG
CCAGGCTTATCAAGGAGGACGTCTACCTCTCCCATGACCACAATATCCCC
TACAAGTGGACGGCCCCTGAAGCGCTCTCCCGAGGCCATTACTCCACCAA
ATCCGACGTCTGGTCCTTTGGGATTCTCCTGCATGAGATGTTCAGCAGGG
GTCAGGTGCCCTACCCAGGCATGTCCAACCATGAGGCCTTCCTGAGGGTG
GACGCCGGCTACCGCATGCCCTGCCCTCTGGAGTGCCCGCCCAGCGTGCA
CAAGCTGATGCTGACATGCTGGTGCAGGGACCCCGAGCAGAGACCCTGCT
TCAAGGCCCTGCGGGAGAGGCTCTCCAGCTTCACCAGCTACGAGAACCCG
ACCTGAGAATTCCCGGGTCGACTCGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMVSRDQAHLGPKYVGLWDFKSR
TDEELSFRAGDVFHVARKEEQWWWATLLDEAGGAVAQGYVPHNYLAERET
VESEPWFFGCISRSEAVRRLQAEGNATGAFLIRVSEKPSADYVLSVRDTQ
AVRHYKIWRRAGGRLHLNEAVSFLSLPELVNYHRAQSLSHGLRLAAPCRK
HEPEPLPHWDDWERPREEFTLCRKLGSGYFGEVFEGLWKDRVQVAIKVIS
RDNLLHQQMLQSEIQAMKKLRHKHILALYAVVSVGDPVYIITELMAKGSL
LELLRDSDEKVLPVSELLDIAWQVAEGMCYLESQNYIHRDLAARNILVGE
NTLCKVGDFGLARLIKEDVYLSHDHNIPYKWTAPEALSRGHYSTKSDVWS
FGILLHEMFSRGQVPYPGMSNHEAFLRVDAGYRMPCPLECPPSVHKLMLT
CWCRDPEQRPCFKALRERLSSFTSYENPT*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5916
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 55203 Daltons