cDNA Clone ABL

• Plasmid: pFastBac.HTb ABL1 118-535
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-ABL1 118-535
• Genbank: NM_005157
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCCCAGTCAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTATCTGCTGAGCAGCGGGATCAATGGCAGCTTCTTGGTGCGTGAGAGTGAGAGCAGTCCTGGCCAGAGGTCCATCTCGCTGAGATACGAAGGGAGGGTGTACCATTACAGGATCAACACTGCTTCTGATGGCAAGCTCTACGTCTCCTCCGAGAGCCGCTTCAACACCCTGGCCGAGTTGGTTCATCATCATTCAACGGTGGCCGACGGGCTCATCACCACGCTCCATTATCCAGCC
CCAAAGCGCAACAAGCCCACTGTCTATGGTGTGTCCCCCAACTACGACAA
GTGGGAGATGGAACGCACGGACATCACCATGAAGCACAAGCTGGGCGGGG
GCCAGTACGGGGAGGTGTACGAGGGCGTGTGGAAGAAATACAGCCTGACG
GTGGCCGTGAAGACCTTGAAGGAGGACACCATGGAGGTGGAAGAGTTCTT
GAAAGAAGCTGCAGTCATGAAAGAGATCAAACACCCTAACCTGGTGCAGC
TCCTTGGGGTCTGCACCCGGGAGCCCCCGTTCTATATCATCACTGAGTTC
ATGACCTACGGGAACCTCCTGGACTACCTGAGGGAGTGCAACCGGCAGGA
GGTGAACGCCGTGGTGCTGCTGTACATGGCCACTCAGATCTCGTCAGCCA
TGGAGTACCTGGAGAAGAAAAACTTCATCCACAGAGATCTTGCTGCCCGA
AACTGCCTGGTAGGGGAGAACCACTTGGTGAAGGTAGCTGATTTTGGCCT
GAGCAGGTTGATGACAGGGGACACCTACACAGCCCATGCTGGAGCCAAGT
TCCCCATCAAATGGACTGCACCCGAGAGCCTGGCCTACAACAAGTTCTCC
ATCAAGTCCGACGTCTGGGCATTTGGAGTATTGCTTTGGGAAATTGCTAC
CTATGGCATGTCCCCTTACCCGGGAATTGACCTGTCCCAGGTGTATGAGC
TGCTAGAGAAGGACTACCGCATGGAGCGCCCAGAAGGCTGCCCAGAGAAG
GTCTATGAACTCATGCGAGCATGTTGGCAGTGGAATCCCTCTGACCGGCC
CTCCTTTGCTGAAATCCACCAAGCCTTTGAAACAATGTTCCAGGAATCCA
GTATCTCAGACGAAGTGGAAAAGGAGCTGGGGAAACAAGGCGTCCGTGGG
GCTGTGAGTACCTTGCTGCAGGCCCCAGAGCTGCCCACCAAGACGAGGAC
CTCCTAGGAATTCCCGGGTCGACTCGAGCGGCCG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSPVNSLEKHSWYHGPVSRNAAEY
LLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSE
SRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMER
TDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAV
MKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVV
LLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMT
GDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSP
YPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEI
HQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5883
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 51018 Daltons