cDNA Clone CEBP (mouse)

• Plasmid: pEBG6P-1 CEBP
• Parent Plasmid: pEBG6P
• Genbank: NM_009883

Sequence of Insert

GGATCCATGCACCGCCTGCTGGCCTGGGACGCAGCATGCCTCCCGCCGCCGCCCGCCGCCTTTAGACCCATGGAAGTGGCCAACTTCTACTACGAGCCCGACTGCCTGGCCTACGGGGCCAAGGCGGCCCGCGCCGCGCCGCGCGCCCCCGCCGCCGAGCCGGCCATTGGCGAGCACGAGCGCGCCATCGACTTCAGCCCCTACCTGGAGCCGCTCGCGCCCGCCGCGGACTTCGCCGCGCCCGCGCCCGCGCACCACGACTTCCTCTCCGACCTCTTCGCCGACGACTACGGCGCCAAGCCGAGC
AAGAAGCCGGCCGACTACGGTTACGTGAGCCTCGGCCGCGCGGGCGCCAA
GGCCGCGCCGCCCGCCTGCTTCCCGCCGCCGCCTCCCGCCGCGCTCAAGG
CGGAGCCGGGCTTCGAACCCGCGGACTGCAAGCGCGCGGACGACGCGCCC
GCCATGGCGGCCGGTTTCCCGTTCGCCCTGCGCGCCTACCTGGGCTACCA
GGCGACGCCGAGCGGCAGCAGCGGCAGCCTGTCCACGTCGTCGTCGTCCA
GCCCGCCCGGCACGCCGAGCCCCGCCGACGCCAAGGCCGCGCCCGCCGCC
TGCTTCGCGGGGCCGCCGGCCGCGCCCGCCAAGGCCAAGGCCAAGAAGAC
GGTGGACAAGCTGAGCGACGAGTACAAGATGCGGCGCGAGCGCAACAACA
TCGCGGTGCGCAAGAGCCGCGACAAGGCCAAGATGCGCAACCTGGAGACG
CAGCACAAGGTGCTGGAGCTGACGGCGGAGAACGAGCGGCTGCAGAAGAA
GGTGGAGCAGCTGTCGCGAGAGCTCAGCACCCTGCGGAACTTGTTCAAGC
AGCTGCCCGAGCCGCTGCTGGCCTCGGCGGGCCACTGCTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMHRLLAWDAACLP
PPPAAFRPMEVANFYYEPDCLAYGAKAARAAPRAPAAEPAIGEHERAIDF
SPYLEPLAPAADFAAPAPAHHDFLSDLFADDYGAKPSKKPADYGYVSLGR
AGAKAAPPACFPPPPPAALKAEPGFEPADCKRADDAPAMAAGFPFALRAY
LGYQATPSGSSGSLSTSSSSSPPGTPSPADAKAAPAACFAGPPAAPAKAK
AKKTVDKLSDEYKMRRERNNIAVRKSRDKAKMRNLETQHKVLELTAENER
LQKKVEQLSRELSTLRNLFKQLPEPLLASAGHC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5836
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express C/EBP beta with N-terminal GST (contains preScission protease site); N.B. Sequence difference compared with NM_009883 CDS:; g390c (silent); ; Estimated MW of expressed protein : 58859 Daltons