cDNA Clone CHOP

• Plasmid: pHR-SIN-CSGW ER-FLAG-CHOP (lent)
• Parent Plasmid: pHR SIN CSGW
• Expressed: ER-FLAG-CHOP
• Genbank: NM_007956 for Estrogen receptor (Mus musculus estrogen receptor 1 (alpha)) S62138 for CHOP
• Tag: FLAG

Sequence of Insert

ACTAGTGCCACCATGCGAAATGAAATGGGTGCTTCAGGAGACATGAGGGCTGCCAACCTTTGGCCAAGCCCTCTTGTGATTAAGCACACTAAGAAGAATAGCCCTGCCTTGTCCTTGACAGCTGACCAGATGGTCAGTGCCTTGTTGGATGCTGAACCGCCCATGATCTATTCTGAATATGATCCTTCTAGACCCTTCAGTGAAGCCTCAATGATGGGCTTATTGACCAACCTAGCAGATAGGGAGCTGGTTCATATGATCAACTGGGCAAAGAGAGTGCCAGGCTTTGGGGACTTGAATCTCCATGATCAGGTCCACCTTCTCGAGTGTGCCTGGCTGGAGATTCTGATGATTGGTCTCGTCTGGCGCTCCATGGAACACCCGGGGAAGCTCCTGTTTGCTCCTAACTTGCTCCTGGACAGGAATCAAGGTAAATGTGTGGAAGGCATGGTGGAGATCTTTGACATGTTGCTTGCTACGTCAAGTCGGTTCCGCATGATGAACCTGCAGGGTGAAGAGTTTGTGTGCCTCAAATCCATCATTTTGCTTAATTCCGGAGTGTACACGTTTCTGTCCAGCACCTTGAAGTCTCTGGAAGAGAAGGACCACATCCACCGTGTCCTGGACAAGATCACAGACACTTTGATCCACCTGATGGCCAAAGCTGGCCTGACTCTGCAGCAGCAGCATCGCCGCCTAGCTCAGCTCCTTCTCATTCTTTCCCATATCCGGCATATGAGTAACAAACGCATGGAGCATCTCTAC
AACATGAAATGCAAGAACGTGGTACCCCTCTATGACCTGCTCCTGGAGAT
GTTGGATGCCCACCGCCTTCATGCCCCAGCCAGTCGCATGGGAGTGCCCC
CAGAGGAGCCCAGCCAGACCCAGCTGGCCACCACCAGCTCCACTTCAGCA
CATTCCTTACAAACCTACTACATACCCCCGGAAGCAGAGGGCTTCCCCAA
CACGATCACTAGT

Amino Acid Sequence

MRNEMGASGDMRAANLWPSPLVIKHTKKNSPALSLTADQMVSALLDAEPP
MIYSEYDPSRPFSEASMMGLLTNLADRELVHMINWAKRVPGFGDLNLHDQ
VHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIF
DMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHI
HRVLDKITDTLIHLMAKAGLTLQQQHRRLAQLLLILSHIRHMSNKGMEHL
YNMKCKNVVPLYDLLLEMLDAHRLHAPASRMGVPPEEPSQTQLATTSSTS
AHSLQTYYIPPEAEGFPNTITSATMDYKDDDDKVFKKEVYLHTSPHLKADVLFQTDPTAEMAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQGRTRKRKQSGHSPARAGKQRMKEKEQENERKVAQLAEENERLKQEIERLTREVEATRRALIDRMVNLHQA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5795
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Species : ; Mouse ER; Human CHOP; ; Plasmid for lentiviral expression in mammalian cells; (SFFV promoter); N.B. Sequence differences in ER CDS compared with NM_007956: ; g1299t (sil), a1338t (sil), c1560t (sil), g1573c (G525R), t1611g (sil), g1614a (sil)