cDNA Clone IPS (Arabidopsis)

• Plasmid: pEBG6P-1 At. IPS
• Parent Plasmid: pEBG6P
• Genbank: NM_120143
• Species: Arabidopsis thaliana

Sequence of Insert

ACTAGTATGTTTATTGAGAGCTTCAAAGTTGAGAGCCCGAACGTGAAGTACACAGAGAATGAGATTCACTCTGTGTACGATTACGAGACGACGGAGGTTGTTCATGAGAAGACCGTCAATGGCACTTACCAATGGATTGTGAAGCCAAAGACTGTCAAATACGATTTCAAAACCGATATTCGTGTCCCCAAATTAGGGGTTATGCTTGTGGGTTTGGGAGGAAACAATGGATCAACTCTCACCGCTGGTGTGATTGCGAACAAAGAAGGAATCTCGTGGGCAACGAAGGACAAAGTGCAACAAGCG
AATTACTTCGGGTCACTAACTCAAGCTTCATCGATTCGTGTCGGATCTTT
TAACGGTGAAGAGATCTATGCTCCTTTCAAGAGTCTCCTTCCAATGGTGA
ATCCGGATGATGTTGTGTTTGGAGGATGGGATATAAGTGACATGAACTTA
GCAGATGCCATGGCTAGAGCCAGGGTTCTTGATATCGACTTGCAGAAACA
GCTCAGGCCTTACATGGAGAACATTGTGCCACTCCCTGGGATCTTCGATC
CTGATTTCATAGCTGCTAATCAAGGGTCACGTGCTAACCACGTGATCAAA
GGTACCAAGAAGGAACAAGTCGACCACATCATCAAGGACATGAGGGAGTT
TAAGGAGAAGAATAAGGTGGATAAGGTTGTGGTTCTCTGGACGGCTAACA
CAGAGCGTTATAGCAATGTGGTCGTTGGGATGAACGATACAATGGAGAAT
CTCATGGAGTCTGTGGATAGGGATGAGGCTGAGATCTCTCCTTCAACACT
TTATGCGATTGCTTGTGTTCTTGAGGGAATCCCGTTCATCAATGGAAGCC
CTCAAAACACCTTTGTTCCAGGTCTAATTGATATGGCGATCAGGAACAAT
GTTTTGATCGGTGGAGATGATTTCAAGAGTGGTCAAACCAAGATGAAATC
TGTCTTGGTTGATTTCCTAGTTGGCGCAGGAATCAAGCCTACTTCAATTG
TGAGCTACAATCACTTGGGAAACAACGATGGAATGAATCTCTCAGCTCCA
CAGACTTTCAGATCTAAGGAGATCTCCAAAAGCAATGTCGTGGACGATAT
GGTTGCTAGCAATGGTATCCTCTTTGAGCCTGGGGAACACCCGGACCATG
TTGTTGTCATCAAGTATGTACCGTATGTTGCGGATAGTAAGCGAGCCATG
GACGAGTACACATCAGAGATATTCATGGGAGGGAAGAACACAATTGTGAT
GCACAATACCTGCGAGGACTCTCTCTTAGCTGCTCCAATCATCTTGGATC
TTGTTCTCCTCGCGGAGCTCAGCACCAGGATCCAGTTCAAATCCGAGGGA
GAGGGAAAGTTTCACTCTTTCCATCCTGTGGCCACCATACTCAGCTACCT
CACCAAGGCACCACTTGTACCGCCGGGAACTCCGGTGATTAACGCACTGT
CGAAGCAGCGGGCTATGCTGGAGAATATTATGAGGGCGTGCGTTGGACTG
GCTCCGGAGAACAACATGATCATGGAATTCAAGTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMFIESFKVESP
NVKYTENEIHSVYDYETTEVVHEKTVNGTYQWIVKPKTVKYDFKTDIRVP
KLGVMLVGLGGNNGSTLTAGVIANKEGISWATKDKVQQANYFGSLTQASS
IRVGSFNGEEIYAPFKSLLPMVNPDDVVFGGWDISDMNLADAMARARVLD
IDLQKQLRPYMENIVPLPGIFDPDFIAANQGSRANHVIKGTKKEQVDHII
KDMREFKEKNKVDKVVVLWTANTERYSNVVVGMNDTMENLMESVDRDEAE
ISPSTLYAIACVLEGIPFINGSPQNTFVPGLIDMAIRNNVLIGGDDFKSG
QTKMKSVLVDFLVGAGIKPTSIVSYNHLGNNDGMNLSAPQTFRSKEISKS
NVVDDMVASNGILFEPGEHPDHVVVIKYVPYVADSKRAMDEYTSEIFMGG
KNTIVMHNTCEDSLLAAPIILDLVLLAELSTRIQFKSEGEGKFHSFHPVA
TILSYLTKAPLVPPGTPVINALSKQRAMLENIMRACVGLAPENNMIMEFK
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5693
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express At. IPS with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 84118 Daltons