cDNA Clone NUAK1

• Plasmid: pEBG-2T-NUAK1 Y270-L320
• Parent Plasmid: pEBG2T
• Expressed: GST-NUAK1 Y270-L320
• Genbank: NM_014840
• Tag: GST
• Species: Human

Sequence of Insert

ACTAGTTACCGGGAGCCAACACAGCCCTCAGATGCTCGAGGACTCATACG
GTGGATGCTGATGGTGAACCCCGATCGCCGGGCCACTATTGAGGACATTG
CCAACCACTGGTGGGTGAACTGGGGCTATAAGAGCAGCGTGTGTGACTGT
GATGCCCTCTAGGACTCTGAGTCCCCACTCCTGGCTCGGATCATTGACTG
GCACCACCGTTCCACAGGGCTGCAGGCTGACACCGAAGCCAAAATGAAGG
GCCTGGCCAAACCCACGACCTCTGAGGTCATGCTAGAGCGGCAGCGGTCG
CTGAAGAAATCCAAGAAAGAGAATGACTTTGCTCAGTCTGGTCAGGATGC
AGTGCCTGAAAGCCCATCCAAGTTGAGTTCTAAGAGGCCCAAGGGGATCC
TGAAGAAGCGAAGCAACAGCGAGCATCGCTCTCACAGCACTGGCTTCATT
GAAGGTGTAGTTGGTCCTGCCTTACCCTCTACTTTCAAGATGGAGCAGGA
CTTGTGCAGGACTGGCGTGCTCCTCCCAAGCTCACCAGAGGCAGAGGTGC
CGGGAAAACTCAGCCCCAAGCAGTCGGCCACGATGCCCAAGAAAGGCATC
TTGAAAAAGACCCAGCAGAGAGAATCAGGTTACTACTCTTCCCCAGAGCG
CAGTGAGTCTTCGGAGCTGTTGGACAGTAATGATGTGATGGGCAGCAGCA
TCCCCTCCCCCAGCCCCCCGGACCCAGCCAGGGTAACCTCCCACAGCCTC
TCCTGCCGGAGGAAGGGCATCTTGAAACACAGCAGCAAATACTCAGCGGG
CACCATGGACCCAGCCCTGGTCAGCCCTGAAATGCCCACACTGGAATCCC
TGTCAGAGCCTGGTGTCCCTGCCGAGGGCCTCTCCCGGAGCTACAGCCGC
CCTTCCAGTGTCATCAGCGATGACAGCGTGCTGTCCAGCGACTCTTTTGA
CTTGCTGGATTTGCAGGAGAATCGCCCTGCCCGCCAGCGCATCCGCAGCT
GCGTCTCTGCAGAAAACTTCCTCCAGATCCAGGACTTTGAGGGGCTCCAG
AACCGGCCCCGGCCCCAGTACCTGAAGCGGTACC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSTSYREPTQPSDARGLIRWMLMVNP
DRRATIEDIANHWWVNWGYKSSVCDCDAL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5622
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express NUAK1 Y270-L320 with N-terminal GST.; ; Estimated MW of expressed protein : 32501 Daltons