cDNA Clone FUS

• Plasmid: pEBG6P-1 FUS
• Parent Plasmid: pEBG6P
• Expressed: GST-FUS
• Genbank: NM_004960
• Tag: GST
• Species: Human

Sequence of Insert

ggatccatggcctcaaacgattatacccaacaagcaacccaaagctatggggcctaccccacccagcccgggcagggctattcccagcagagcagtcagccctacggacagcagagttacagtggttatagccagtccacggacacttcaggctatggccagagcagctattcttcttatggccagagccagaacacaggctatggaactcagtcaactccccagggatatggctcgactggcggctatggcagtagccagagctcccaatcgtcttacgggcagcagtcctcctatcctggctat
ggccagcagccagctcccagcagcacctcgggaagttacggtagcagttc
tcagagcagcagctatgggcagccccagagtgggagctacagccagcagc
ctagctatggtggacagcagcaaagctatggacagcagcaaagctataat
ccccctcagggctatggacagcagaaccagtacaacagcagcagtggtgg
tggaggtggaggtggaggtggaggtaactatggccaagatcaatcctcca
tgagtagtggtggtggcagtggtggcggttatggcaatcaagaccagagt
ggtggaggtggcagcggtggctatggacagcaggaccgtggaggccgcgg
caggggtggcagtggtggcggcggcggcggcggcggtggtggttacaacc
gcagcagtggtggctatgaacccagaggtcgtggaggtggccgtggaggc
agaggtggcatgggcggaagtgaccgtggtggcttcaataaatttggtgg
ccctcgggaccaaggatcacgtcatgactccgaacaggataattcagaca
acaacaccatctttgtgcaaggcctgggtgagaatgttacaattgagtct
gtggctgattacttcaagcagattggtattattaagacaaacaagaaaac
gggacagcccatgattaatttgtacacagacagggaaactggcaagctga
agggagaggcaacggtctcttttgatgacccaccttcagctaaagcagct
attgactggtttgatggtaaagaattctccggaaatcctatcaaggtctc
atttgctactcgccgggcagactttaatcggggtggtggcaatggtcgtg
gaggccgagggcgaggaggacccatgggccgtggaggctatggaggtggt
ggcagtggtggtggtggccgaggaggatttcccagtggaggtggtggcgg
tggaggacagcagcgagctggtgactggaagtgtcctaatcccacctgtg
agaatatgaacttctcttggaggaatgaatgcaaccagtgtaaggcccct
aaaccagatggcccaggagggggaccaggtggctctcacatggggggtaa
ctacggggatgatcgtcgtggtggcagaggaggctatgatcgaggcggct
accggggccgcggcggggaccgtggaggcttccgagggggccggggtggt
ggggacagaggtggctttggccctggcaagatggattccaggggtgagca
cagacaggatcgcagggagaggccgtattaagcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMASNDYTQQATQS
YGAYPTQPGQGYSQQSSQPYGQQSYSGYSQSTDTSGYGQSSYSSYGQSQN
TGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAPSSTSGSY
GSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNS
SSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDR
GGRGRGGSGGGGGGGGGGYNRSSGGYEPRGRGGGRGGRGGMGGSDRGGFN
KFGGPRDQGSRHDSEQDNSDNNTIFVQGLGENVTIESVADYFKQIGIIKT
NKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGNP
IKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSG
GGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSH
MGGNYGDDRRGGRGGYDRGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDS
RGEHRQDRRERPY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5568
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express FUS with N-terminal GST (contains preScission protease site); Sequence difference compared with NM_004960: c291t (silent in Y97).; ; Estimated MW of expressed protein : 80842 Daltons