cDNA Clone LMO7

• Plasmid: pGEX6P-3-LMO7 417-end
• Parent Plasmid: pGEX6P3
• Expressed: GST-LMO7 417-end
• Genbank: NM_005358
• Tag: GST
• Species: Human

Sequence of Insert

gtcgacAGTTACACTTCAGATCTGCAGAAGAAAAAAGAAGAGAGAGAAGAAATTGAAAAGCAGGCACTTGAGAAGTCTAAGAGAAGCTCTAAGACGTTTAAGGAAATGCTGCAGGACAGGGAATCCCAAAATCAAAAGTCTACAGTTCCGTCAAGAAGGAGAATGTATTCTTTTGATGATGTGCTGGAGGAAGGAAAGCGACCCCCTACAATGACTGTGTCAGAAGCAAGTTACCAGAGTGAGAGAGTAGAAGAGAAGGGAGCAACTTATCCTTCAGAAATTCCCAAAGAAGATTCTACCACTTTT
GCAAAAAGAGAGGACCGTGTAACAACTGAAATTCAGCTTCCTTCTCAAAG
TCCTGTGGAAGAACAAAGCCCAGCCTCTTTGTCTTCTCTGCGTTCACGGA
GCACACAAATGGAATCAACTTGTGTTTCAGCTTCTCTCCCCAGAAGTTAC
CGGAAAACTGATACAGTCAGGTTAACATCTGTGGTCACACCAAGACCCTT
TGGCTCTCAGACAAGGGGAATCTCATCACTCCCCAGATCTTACACGATGG
ATGATGCTTGGAAGTATAATGGAGATATTGAAGACATTAAGAGAACTCCA
AACAATGTGGTCAGCACCCCTGCACCAAGCCCGGACGCAAGCCAACTGGC
TTCAAGCTTATCTAGCCAGAAAGAGGTAGCAGCAACAGAAGAAGATGTGA
CAAGGCTGCCCTCTCCTACATCCCCCTTCTCATCTCTTTCCCAAGACCAG
GCTGCCACTTCTAAAGCCACATTGTCTTCCACATCTGGTCTTGATTTAAT
GTCTGAATCTGGAGAAGGGGAAATCTCCCCACAAAGAGAAGTCTCAAGAT
CCCAGGATCAGTTCAGTGATATGAGAATCAGCATAAACCAGACGCCTGGG
AAGAGTCTTGACTTTGGGTTTACAATAAAATGGGATATTCCTGGGATCTT
CGTAGCATCAGTTGAAGCAGGTAGCCCAGCAGAATTTTCTCAGCTACAAG
TAGATGATGAAATTATTGCTATTAACAACACCAAGTTTTCATATAACGAT
TCAAAAGAGTGGGAGGAAGCCATGGCTAAGGCTCAAGAAACTGGACACCT
AGTGATGGATGTGAGGCGCTATGGAAAGGCTGGTTCACCTGAAACAAAGT
GGATTGATGCAACTTCTGGAATTTACAACTCAGAAAAATCTTCAAATCTA
TCTGTAACAACTGATTTCTCCGAAAGCCTTCAGAGTTCTAATATTGAATC
CAAAGAAATCAATGGAATTCATGATGAAAGCAATGCTTTTGAATCAAAAG
CATCTGAATCCATTTCTTTGAAAAACTTAAAAAGGCGATCACAATTTTTT
GAACAAGGAAGCTCTGATTCGGTGGTTCCTGATCTTCCAGTTCCAACCAT
CAGTGCCCCGAGTCGCTGGGTGTGGGATCAAGAGGAGGAGCGGAAGCGGC
AGGAGAGGTGGCAGAAGGAGCAGGACCGCCTACTGCAGGAAAAATATCAA
CGTGAGCAGGAGAAACTGAGGGAAGAGTGGCAAAGGGCCAAACAGGAGGC
AGAGAGAGAGAATTCCAAGTACTTGGATGAGGAACTGATGGTCCTAAGCT
CAAACAGCATGTCTCTGACCACACGGGAGCCCTCTCTTGCCACCTGGGAA
GCTACCTGGAGTGAAGGGTCCAAGTCTTCAGACAGAGAAGGAACCCGAGC
AGGAGAAGAGGAGAGGAGACAGCCACAAGAGGAAGTTGTTCATGAGGACC
AAGGAAAGAAGCCGCAGGATCAGCTTGTTATTGAGAGAGAGAGGAAATGG
GAGCAACAGCTTCAGGAAGAGCAAGAGCAAAAGCGGCTTCAGGCTGAGGC
TGAGGAGCAGAAGCGTCCTGCGGAGGAGCAGAAGCGCCAGGCAGAGATAG
AGCGGGAAACATCAGTCAGAATATACCAGTACAGGAGGCCTGTTGATTCC
TATGATATACCAAAGACAGAAGAAGCATCTTCAGGTTTTCTTCCTGGTGA
CAGGAATAAATCCAGATCTACTACTGAACTGGATGATTACTCCACAAATA
AAAATGGAAACAATAAATATTTAGACCAAATTGGGAACACGACCTCTTCA
CAGAGGAGATCCAAGAAAGAACAAGTACCATCAGGAGCAGAATTGGAGAG
GCAACAAATCCTTCAGGAAATGAGGAAGAGAACACCCCTTCACAATGACA
ACAGCTGGATCCGACAGCGCAGTGCCAGTGTCAACAAAGAGCCTGTTAGT
CTTCCTGGGATCATGAGAAGAGGCGAATCTTTAGATAACCTGGACTCCCC
CCGATCCAATTCTTGGAGACAGCCTCCTTGGCTCAATCAGCCCACAGGAT
TCTATGCTTCTTCCTCTGTGCAAGACTTTAGTCGCCCACCACCTCAGCTG
GTGTCCACATCAAACCGTGCCTACATGCGGAACCCCTCCTCCAGCGTGCC
CCCACCTTCAGCTGGCTCCGTGAAGACCTCCACCACAGGTGTGGCCACCA
CACAGTCCCCCACCCCGAGAAGCCATTCCCCTTCAGCTTCACAGTCAGGC
TCTCAGCTGCGTAACAGGTCAGTCAGTGGGAAGCGCATATGCTCCTACTG
CAATAACATTCTGGGCAAAGGAGCCGCCATGATCATCGAGTCCCTGGGTC
TTTGTTATCATTTGCATTGTTTTAAGTGTGTTGCCTGTGAGTGTGACCTC
GGAGGCTCTTCCTCAGGAGCTGAAGTCAGGATCAGAAACCACCAACTGTA
CTGCAACGACTGCTATCTCAGATTCAAATCTGGACGGCCAACCGCCATGT
GAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDSYTSDLQKKKEER
EEIEKQALEKSKRSSKTFKEMLQDRESQNQKSTVPSRRRMYSFDDVLEEG
KRPPTMTVSEASYQSERVEEKGATYPSEIPKEDSTTFAKREDRVTTEIQL
PSQSPVEEQSPASLSSLRSRSTQMESTCVSASLPRSYRKTDTVRLTSVVT
PRPFGSQTRGISSLPRSYTMDDAWKYNGDIEDIKRTPNNVVSTPAPSPDA
SQLASSLSSQKEVAATEEDVTRLPSPTSPFSSLSQDQAATSKATLSSTSG
LDLMSESGEGEISPQREVSRSQDQFSDMRISINQTPGKSLDFGFTIKWDI
PGIFVASVEAGSPAEFSQLQVDDEIIAINNTKFSYNDSKEWEEAMAKAQE
TGHLVMDVRRYGKAGSPETKWIDATSGIYNSEKSSNLSVTTDFSESLQSS
NIESKEINGIHDESNAFESKASESISLKNLKRRSQFFEQGSSDSVVPDLP
VPTISAPSRWVWDQEEERKRQERWQKEQDRLLQEKYQREQEKLREEWQRA
KQEAERENSKYLDEELMVLSSNSMSLTTREPSLATWEATWSEGSKSSDRE
GTRAGEEERRQPQEEVVHEDQGKKPQDQLVIERERKWEQQLQEEQEQKRL
QAEAEEQKRPAEEQKRQAEIERETSVRIYQYRRPVDSYDIPKTEEASSGF
LPGDRNKSRSTTELDDYSTNKNGNNKYLDQIGNTTSSQRRSKKEQVPSGA
ELERQQILQEMRKRTPLHNDNSWIRQRSASVNKEPVSLPGIMRRGESLDN
LDSPRSNSWRQPPWLNQPTGFYASSSVQDFSRPPPQLVSTSNRAYMRNPS
SSVPPPSAGSVKTSTTGVATTQSPTPRSHSPSASQSGSQLRNRSVSGKRI
CSYCNNILGKGAAMIIESLGLCYHLHCFKCVACECDLGGSSSGAEVRIRN
HQLYCNDCYLRFKSGRPTAM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5547
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. sequence difference compared with NM_005358: ; c1669t (R557C), g1825a (V609I), t3338c (M1113T)- in several ests, other mRNA seqs, and rat and mouse seqs; a2304g (silent K768); ; Estimated MW of expressed protein : 132772 Daltons