cDNA Clone MSK1

• Synonyms: RPS6KA5
• Plasmid: pVAh-CD2-FLAG-MSK1 581D 700D
• Parent Plasmid: pVAhCD2-Age1
• Expressed: FLAG-MSK1 581D 700D
• Genbank: AF074393
• Tag: FLAG
• Species: Human

Sequence of Insert

gaattcGCCACCATGGACTACAAGGACGACGATGACAAGGAGGAGGAGGG
TGGCAGCAGCGGCGGCGCCGCGGGGACCAGCGCGGACGGCGGCGACGGAG
GAGAGCAGCTCCTCACTGTCAAGCACGAGCTGCGGACTGCTAATTTGACA
GGACATGCTGAGAAGGTGGGAATAGAAAATTTTGAGCTCCTGAAGGTCCT
AGGAACTGGAGCTTATGGAAAAGTATTTCTAGTTCGTAAAATAAGTGGCC
ATGATACTGGAAAGCTGTATGCCATGAAAGTTTTGAAAAAGGCAACAATC
GTTCAAAAGGCCAAAACCACAGAGCATACAAGGACAGAACGACAAGTCCT
GGAACACATTAGGCAGTCGCCATTTTTGGTAACATTACATTATGCTTTCC
AGACAGAAACCAAACTTCATCTCATTTTAGATTATATAAATGGTGGTGAA
CTTTTTACTCATCTTTCTCAAAGAGAGCGTTTCACAGAGCATGAGGTGCA
GATTTATGTTGGAGAGATTGTGCTTGCCCTCGAACATCTCCACAAGTTGG
GGATTATATATCGTGATATTAAGCTTGAGAATATTCTACTTGATTCTAAT
GGCCATGTGGTGCTGACAGATTTTGGTCTGAGTAAGGAGTTTGTGGCTGA
TGAAACTGAAAGAGCATATTCCTTTTGTGGAACTATTGAATACATGGCAC
CAGATATTGTCAGAGGGGGAGATTCAGGACATGACAAGGCAGTTGACTGG
TGGAGTTTGGGTGTTCTAATGTATGAATTACTAACTGGAGCATCTCCTTT
CACTGTTGATGGAGAAAAAAATTCCCAAGCTGAGATATCTAGGAGAATAT
TAAAAAGTGAGCCTCCATATCCCCAAGAAATGAGTGCTTTAGCGAAAGAC
CTAATTCAGCGTCTTTTGATGAAAGATCCCAAGAAGAGATTGGGATGTGG
TCCACGTGATGCAGATGAAATCAAAGAACATCTCTTCTTTCAGAAAATAA
ATTGGGATGATTTAGCCGCCAAAAAAGTGCCTGCACCATTTAAGCCAGTC
ATTCGAGATGAATTAGATGTGAGTAACTTTGCAGAAGAGTTCACAGAAAT
GGATCCCACTTATTCTCCCGCAGCCCTGCCCCAGAGTTCTGAGAAGCTGT
TTCAGGGCTATTCCTTTGTTGCTCCTTCCATCCTATTCAAGCGTAATGCA
GCTGTCATAGACCCTCTTCAGTTTCACATGGGAGTTGAACGTCCTGGAGT
GACAAATGTTGCCAGGAGTGCAATGATGAAGGACTCTCCATTCTATCAAC
ACTATGACCTAGATTTGAAGGACAAACCCCTGGGAGAAGGTAGTTTTTCA
ATTTGTCGAAAGTGTGTGCATAAAAAAAGTAACCAAGCTTTTGCAGTCAA
AATAATCAGCAAAAGGATGGAAGCCAATACTCAAAAGGAAATAACAGCTC
TGAAACTCTGTGAAGGACACCCCAATATTGTGAAGTTGCATGAAGTTTTT
CATGATCAGCTTCACACGTTTCTAGTGATGGAACTTCTGAATGGAGGAGA
ACTGTTTGAGCGCATTAAGAAAAAGAAGCACTTCAGTGAGACGGAAGCCA
GCTACATCATGAGGAAGCTTGTTTCAGCTGTAAGCCACATGCATGATGTT
GGAGTGGTGCACAGGGATCTGAAACCTGAGAATTTATTGTTCACCGATGA
AAATGACAATTTGGAAATTAAAATAATTGATTTTGGATTTGCACGGCTAA
AGCCACCGGATAATCAGCCCCTGAAGGATCCATGCTTCACCCTTCATTAT
GCCGCCCCAGAGCTCTTGAATCAGAACGGCTACGATGAGTCCTGTGACCT
GTGGAGCTTGGGCGTCATTTTGTACACAATGTTGTCAGGACAGGTTCCCT
TCCAATCTCATGACCGAAGTTTGACGTGTACCAGCGCGGTGGAAATCATG
AAGAAAATTAAAAAGGGAGATTTCTCCTTTGAAGGAGAAGCCTGGAAGAA
TGTATCCCAAGAGGCTAAAGATTTGATCCAAGGACTTCTCACAGTAGATC
CAAACAAAAGGCTTAAAATGTCTGGCTTGAGGTACAATGAATGGCTACAA
GATGGAAGTCAGCTGTCCTCCAATCCTCTGATGGATCCGGATATTCTAGG
ATCTTCCGGAGCTGCCGTGCATACCTGTGTGAAAGCAACCTTCCACGCCT
TTAACAAATACAAGAGAGAGGGGTTTTGCCTTCAGAATGTTGATAAGGCC
CCTTTGGCTAAGAGAAGAAAAATGAAAAAGACTAGCACCAGTACCGAGAC
GCGCAGCAGTTCCAGTGAGAGTTCCCATTCTTCTTCCTCTCATTCTCACG
GTAAAACTACACCCACCAAGACACTGCAGCCCAGCAATCCTGCCGACAGC
AATAACCCGGAGACCCTCTTCCAGTTCTCGGACTCAGTAGCTTAGaccgg
t

Amino Acid Sequence

MDYKDDDDKEEEGGSSGGAAGTSADGGDGGEQLLTVKHELRTANLTGHAE
KVGIENFELLKVLGTGAYGKVFLVRKISGHDTGKLYAMKVLKKATIVQKA
KTTEHTRTERQVLEHIRQSPFLVTLHYAFQTETKLHLILDYINGGELFTH
LSQRERFTEHEVQIYVGEIVLALEHLHKLGIIYRDIKLENILLDSNGHVV
LTDFGLSKEFVADETERAYSFCGTIEYMAPDIVRGGDSGHDKAVDWWSLG
VLMYELLTGASPFTVDGEKNSQAEISRRILKSEPPYPQEMSALAKDLIQR
LLMKDPKKRLGCGPRDADEIKEHLFFQKINWDDLAAKKVPAPFKPVIRDE
LDVSNFAEEFTEMDPTYSPAALPQSSEKLFQGYSFVAPSILFKRNAAVID
PLQFHMGVERPGVTNVARSAMMKDSPFYQHYDLDLKDKPLGEGSFSICRK
CVHKKSNQAFAVKIISKRMEANTQKEITALKLCEGHPNIVKLHEVFHDQL
HTFLVMELLNGGELFERIKKKKHFSETEASYIMRKLVSAVSHMHDVGVVH
RDLKPENLLFTDENDNLEIKIIDFGFARLKPPDNQPLKDPCFTLHYAAPE
LLNQNGYDESCDLWSLGVILYTMLSGQVPFQSHDRSLTCTSAVEIMKKIK
KGDFSFEGEAWKNVSQEAKDLIQGLLTVDPNKRLKMSGLRYNEWLQDGSQ
LSSNPLMDPDILGSSGAAVHTCVKATFHAFNKYKREGFCLQNVDKAPLAK
RRKMKKTSTSTETRSSSSESSHSSSSHSHGKTTPTKTLQPSNPADSNNPE
TLFQFSDSVA*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU5532
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for expression of FLAG.MSK1 T581D T700D in mouse T-cells. Parental vector from D. Cantrell.