cDNA Clone Nor1 (mouse)

• Plasmid: pGEX6P-3-Nor1
• Parent Plasmid: pGEX6P3
• Genbank: NM_015743
• Species: Mouse

Sequence of Insert

GGATCCCCCTGCGTGCAAGCCCAGTATAGCCCTTCACCTCCGGGGTCCACTTACGCCACGCAGACTTATGGCTCGGAATACACCACAGAAATCATGAACCCCGACTACACCAAGCTGACCATGGACCTCGGTAGCACGGGGATCATGGCCACT
GCCACTACATCCCTGCCCAGCTTCAGTACCTTCATGGAGGGCTACCCCAG
CAGCTGCGAACTCAAGCCCTCCTGCCTGTACCAAATGCCGCCTTCTGGGC
CTCGGCCTTTGATCAAGATGGAAGAGGGTCGCGAGCATGGCTACCACCAC
CACCATCACCATCACCATCATCACCACCACCACCAGCAACAGCAGCCGTC
CATTCCTCCTCCCTCCGGCCCCGAGGACGAGGTACTGCCCAGCACCTCCA
TGTACTTCAAGCAGTCTCCGCCTTCTACACCGACCACTCCAGGCTTCCCC
CCGCAGGCGGGGGCGCTGTGGGACGACGAGCTGCCCTCTGCGCCTGGCTG
CATCGCTCCGGGACCGCTGCTGGACCCGCAGATGAAGGCGGTACCCCCCA
TGGCCGCTGCTGCGCGCTTCCCGATCTTCTTCAAGCCCTCACCGCCACAC
CCTCCCGCGCCCAGTCCAGCCGGCGGCCACCACCTCGGCTATGACCCCAC
GGCCGCAGCTGCACTCAGTCTGCCCCTGGGAGCCGCGGCCGCAGCAGGCA
GCCAAGCTGCTGCGCTCGAGGGCCACCCATACGGGCTCCCGCTGGCCAAG
AGGACGGCCACGCTGACCTTCCCTCCGCTGGGCCTCACAGCCTCCCCCAC
CGCGTCCAGCCTGCTGGGAGAGAGCCCCAGCCTCCCATCGCCACCCAATA
GGAGCTCATCATCTGGGGAAGGCACATGTGCCGTGTGCGGCGACAACGCT
GCCTGCCAGCACTACGGAGTCCGCACCTGCGAGGGCTGCAAGGGCTTCTT
CAAGAGAACGGTGCAGAAAAATGCAAAATATGTTTGCCTGGCAAATAAAA
ACTGCCCAGTGGACAAGAGACGCCGAAACCGATGTCAGTACTGCAGATTT
CAGAAGTGTCTCAGTGTCGGGATGGTTAAGGAAGTTGTGCGTACAGACAG
TCTGAAAGGGAGGAGAGGTCGTCTGCCTTCCAAACCAAAGAGCCCACTAC
AACAGGAGCCCTCGCAGCCCTCCCCGCCATCTCCTCCGATCTGTATGATG
AATGCCCTTGTCCGAGCTTTAACAGATGCAACACCCAGAGATCTTGATTA
TTCCAGATACTGTCCCACCGACCAGGCCACTGCAGGCACAGATGCTGAGC
ACGTGCAACAGTTCTACAACCTTCTGACGGCCTCCATTGACGTGTCCAGA
AGCTGGGCAGAAAAGATCCCAGGATTCACTGATCTCCCCAAAGAAGATCA
GACGTTACTTATAGAATCAGCCTTTTTGGAGCTGTTTGTTCTTAGACTTT
CCATCAGGTCAAACACTGCTGAAGATAAGTTTGTGTTCTGCAATGGACTT
GTCCTGCATCGACTTCAGTGCCTTCGAGGATTTGGGGAATGGCTCGACTC
CATTAAAGACTTTTCTTTAAACTTGCAGAGCCTGAACCTTGATATCCAAG
CCTTAGCCTGCCTGTCAGCACTGAGTATGATCACAGAGCGACATGGGTTA
AAAGAACCAAAGAGAGTGGAGGAGCTATGCACCAAGATCACAAGCAGCTT
AAAGGACCACCAGAGGAAGGGACAGGCTCTGGAGCCCTCGGAGCCTAAGG
TCCTGCGCGCGCTGGTAGAACTGAGAAAGATCTGTACCCAGGGCCTCCAG
CGCATCTTCTACCTGAAGCTAGAGGACTTGGTACCTCCGCCTTCTGTCAT
CGACAAGCTCTTCCTTGACACCCTGCCTTTCTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPCVQAQYSPSPPGSTYATQ
TYGSEYTTEIMNPDYTKLTMDLGSTGIMATATTSLPSFSTFMEGYPSSCE
LKPSCLYQMPPSGPRPLIKMEEGREHGYHHHHHHHHHHHHHQQQQPSIPP
PSGPEDEVLPSTSMYFKQSPPSTPTTPGFPPQAGALWDDELPSAPGCIAP
GPLLDPQMKAVPPMAAAARFPIFFKPSPPHPPAPSPAGGHHLGYDPTAAA
ALSLPLGAAAAAGSQAAALEGHPYGLPLAKRTATLTFPPLGLTASPTASS
LLGESPSLPSPPNRSSSSGEGTCAVCGDNAACQHYGVRTCEGCKGFFKRT
VQKNAKYVCLANKNCPVDKRRRNRCQYCRFQKCLSVGMVKEVVRTDSLKG
RRGRLPSKPKSPLQQEPSQPSPPSPPICMMNALVRALTDATPRDLDYSRY
CPTDQATAGTDAEHVQQFYNLLTASIDVSRSWAEKIPGFTDLPKEDQTLL
IESAFLELFVLRLSIRSNTAEDKFVFCNGLVLHRLQCLRGFGEWLDSIKD
FSLNLQSLNLDIQALACLSALSMITERHGLKEPKRVEELCTKITSSLKDH
QRKGQALEPSEPKVLRALVELRKICTQGLQRIFYLKLEDLVPPPSVIDKL
FLDTLPF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5419
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. Sequence differences compared with NM_015743 CDS:; c150t (silent T50), g423t (silent P141), g1539a (silent E513), a1839g (silent P613); (All derive from Nor1 cloned by Mark Peggie); ; Estimated MW of expressed protein : 95085 Daltons