cDNA Clone ILK

• Plasmid: pGEX6P-1-ILK
• Parent Plasmid: pGEX6P1
• Expressed: GST-ILK
• Genbank: NM_004517
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGGACGACATTTTCACTCAGTGCCGGGAGGGCAACGCAGTCGCCGTTCGCCTGTGGCTGGACAACACGGAGAACGACCTCAACCAGGGGGACGATCATGGCTTCTCCCCCTTGCACTGGGCCTGCCGAGAGGGCCGCTCTGCTGTGGTTGAGATGTTGATCATGCGGGGGGCACGGATCAATGTAATGAACCGTGGGGATGACACCCCCCTGCATCTGGCAGCCAGTCATGGACACCGTGATATTGTACAGAAGCTATTGCAGTACAAGGCAGACATCAATGCAGTGAATGAACATGGG
AATGTGCCCCTGCACTATGCCTGTTTTTGGGGCCAAGATCAAGTGGCAGA
GGACCTGGTGGCAAATGGGGCCCTTGTCAGCATCTGTAACAAGTATGGAG
AGATGCCTGTGGACAAAGCCAAGGCACCCCTGAGAGAGCTTCTCCGAGAG
CGGGCAGAGAAGATGGGCCAGAATCTCAACCGTATTCCATACAAGGACAC
ATTCTGGAAGGGGACCACCCGCACTCGGCCCCGAAATGGAACCCTGAACA
AACACTCTGGCATTGACTTCAAACAGCTTAACTTCCTGACGAAGCTCAAC
GAGAATCACTCTGGAGAGCTATGGAAGGGCCGCTGGCAGGGCAATGACAT
TGTCGTGAAGGTGCTGAAGGTTCGAGACTGGAGTACAAGGAAGAGCAGGG
ACTTCAATGAAGAGTGTCCCCGGCTCAGGATTTTCTCGCATCCAAATGTG
CTCCCAGTGCTAGGTGCCTGCCAGTCTCCACCTGCTCCTCATCCTACTCT
CATCACACACTGGATGCCATATGGATCCCTCTACAATGTACTACATGAAG
GCACCAATTTCGTCGTGGACCAGAGCCAGGCTGTGAAGTTTGCTTTGGAC
ATGGCAAGGGGCATGGCCTTCCTACACACACTAGAGCCCCTCATCCCACG
ACATGCACTCAATAGCCGTAGTGTAATGATTGATGAGGACATGACTGCCC
GAATTAGCATGGCTGATGTCAAGTTCTCTTTCCAATGTCCTGGTCGCATG
TATGCACCTGCCTGGGTAGCCCCCGAAGCTCTGCAGAAGAAGCCTGAAGA
CACAAACAGACGCTCAGCAGACATGTGGAGTTTTGCAGTGCTTCTGTGGG
AACTGGTGACACGGGAGGTACCCTTTGCTGACCTCTCCAATATGGAGATT
GGAATGAAGGTGGCATTGGAAGGCCTTCGGCCTACCATCCCACCAGGTAT
TTCCCCTCATGTGTGTAAGCTCATGAAAATCTGCATGAATGAAGACCCTG
CAAAGCGACCCAAATTTGACATGATTGTGCCTATCCTTGAGAAGATGCAG
GACAAGTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMDDIFTQCREGNAVAV
RLWLDNTENDLNQGDDHGFSPLHWACREGRSAVVEMLIMRGARINVMNRG
DDTPLHLAASHGHRDIVQKLLQYKADINAVNEHGNVPLHYACFWGQDQVA
EDLVANGALVSICNKYGEMPVDKAKAPLRELLRERAEKMGQNLNRIPYKD
TFWKGTTRTRPRNGTLNKHSGIDFKQLNFLTKLNENHSGELWKGRWQGND
IVVKVLKVRDWSTRKSRDFNEECPRLRIFSHPNVLPVLGACQSPPAPHPT
LITHWMPYGSLYNVLHEGTNFVVDQSQAVKFALDMARGMAFLHTLEPLIP
RHALNSRSVMIDEDMTARISMADVKFSFQCPGRMYAPAWVAPEALQKKPE
DTNRRSADMWSFAVLLWELVTREVPFADLSNMEIGMKVALEGLRPTIPPG
ISPHVCKLMKICMNEDPAKRPKFDMIVPILEKMQDK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5414
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. Sequence differences compared with NM_004517 CDS:; c297t (silent H99), g819a (silent P273), g1278a (silent K426); ; Estimated MW of expressed protein : 78567 Daltons