cDNA Clone Nur77 (mouse)

• Plasmid: pEBG6P-1 Nur77 S354A
• Parent Plasmid: pEBG6P
• Genbank: X60132
• Species: Mouse

Sequence of Insert

ggatccATGCCCTGTATTCAAGCTCAATATGGAACACCAGCAACGAGCCCAGGACCGCGTGACCACCTGACCGGTGATCCCCTGGCCCTTGAGTTCGGCAAGCCTACCATGGACCTGGCCAGCCCCGAGACAGCACCTACCGCACCTGCTACACTGCCCAGCTTCAGCACCTTCATGGACGGGTACACCGGAGAGTTTGACACCTTCCTCTACCAGCTGCCGGGGACGACCCAGCCGTGCTCCTCAGCTTGTTCCTCTGCCTCCTCCACGTCTTCTTCCTCATCCTCGGCCACCTCCCCCGCTTCG
GCGTCCTTCAAGTTTGAGGACTTCCAGGTGTACGGCTGCTACCCGGGCAC
CCTGAGCGGCCCATTAGATGAGACCCTATCCTCCAGCGGCTCTGAGTACT
ATGGCAGTCCCTGCTCAGCCCCCTCGCCATCTACACCCAACTTCCAGCCG
TCCCAGCTTTCTCCCTGGGACGGCTCATTTGGCCACTTCTCCCCGAGCCA
GACTTATGAAGGCCTCTGGGCATGGACAGAGCAGTTGCCTAAGGCTTCTT
CAGGGCCTCCGCCACCTCCAACCTTCTTCTCCTTCAGTCCTCCCACTGGC
CCCAGCCCCAGCCTGGCCCAGAGTTCTCTGAAATTGTTCCCACCACCAGC
CACCCACCAGCTTGGGGAGGGGGAGAGCTATTCCATGCCAGCAGCTTTCC
CCGGCTTGGCACCCACCTCTCCGAACCGTGACACTTCCGGCATTCTGGAC
GCACCCGTGACCTCCACCAAGTCCCGGAGCGGGGCTTCAGGTGGCAGCGA
GGGCCGCTGTGCAGTCTGTGGTGACAATGCTTCGTGTCAGCACTATGGGG
TCCGCACCTGTGAGGGCTGCAAGGGCTTCTTCAAGCGCACAGTACAGAAA
AGCGCCAAGTACATCTGCCTGGCAAACAAGGATTGCCCTGTGGACAAGAG
GCGGCGGAACCGCTGCCAGTTCTGCCGCTTCCAGAAGTGCCTGGCTGTGG
GCATGGTGAAGGAAGTTGTACGGACAGACAGCCTAAAAGGGCGGCGGGGC
CGGCTACCTGCAAAACCCAAGCAGCCTCCAGATGCCTCCCCTACCAATCT
TCTCACTTCCCTCATCCGGGCACACTTGGACTCCGGGCCTAGCACTGCCA
AATTGGACTATTCCAAGTTCCAGGAACTGGTGCTGCCCCGCTTCGGGAAG
GAAGATGCCGGTGACGTGCAACAATTTTATGACTTGCTCTCTGGTTCCCT
GGACGTTATCCGAAAGTGGGCAGAAAAAATCCCTGGCTTCATTGAGCTTT
GCCCAGGAGACCAAGACCTGTTGCTAGAGTCTGCCTTCCTGGAACTCTTC
ATCCTCCGCCTGGCATACCGATCTAAACCCGGTGAGGGGAAGCTCATCTT
CTGCTCAGGCCTGGTACTACACCAGCTGCAGTGTGCCCGTGGCTTTGGTG
ATTGGATTGACAACATCCTGGCCTTCTCACGGTCCCTGCACAGCTTGGGT
GTTGATGTTCCCGCCTTTGCCTGCCTGTCCGCTCTGGTCCTCATCACTGA
TCGACACGGGCTCCAGGACCCTCGTCGGGTGGAAGAGCTGCAGAATCGCA
TTGCTAGCTGTCTGAAGGAGCACATGGCTACCGTGGCAGGAGACCCACAG
CCGGCCAGCTGCCTGTCACGTCTGCTGGGCAAACTGCCTGAGCTTCGGAC
CCTGTGCACTCAAGGCCTGCAGCGCATCTTTTGCCTCAAGTTGGAGGACT
TGGTACCCCCTCCACCTATTGTGGACAAGATCTTTATGGACACATTGTCT
TTCTGAgcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMPCIQAQYGTPAT
SPGPRDHLTGDPLALEFGKPTMDLASPETAPTAPATLPSFSTFMDGYTGE
FDTFLYQLPGTTQPCSSACSSASSTSSSSSSATSPASASFKFEDFQVYGC
YPGTLSGPLDETLSSSGSEYYGSPCSAPSPSTPNFQPSQLSPWDGSFGHF
SPSQTYEGLWAWTEQLPKASSGPPPPPTFFSFSPPTGPSPSLAQSSLKLF
PPPATHQLGEGESYSMPAAFPGLAPTSPNRDTSGILDAPVTSTKSRSGAS
GGSEGRCAVCGDNASCQHYGVRTCEGCKGFFKRTVQKSAKYICLANKDCP
VDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRGRLPAKPKQPPDAS
PTNLLTSLIRAHLDSGPSTAKLDYSKFQELVLPRFGKEDAGDVQQFYDLL
SGSLDVIRKWAEKIPGFIELCPGDQDLLLESAFLELFILRLAYRSKPGEG
KLIFCSGLVLHQLQCARGFGDWIDNILAFSRSLHSLGVDVPAFACLSALV
LITDRHGLQDPRRVEELQNRIASCLKEHMATVAGDPQPASCLSRLLGKLP
ELRTLCTQGLQRIFCLKLEDLVPPPPIVDKIFMDTLSF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5401
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Nurr77 S354A with N-terminal GST (contains preScission protease site); N.B. Sequence differences compared with X60132 CDS: ; g363a (silent P121); t1060g (S354A); ; Estimated MW of expressed protein : 92111 Daltons