cDNA Clone Nur77 (mouse)

• Plasmid: pcDNA4/TO GFP.Nur77 S354D
• Parent Plasmid: pcDNA4/TO
• Genbank: X60132
• Tag: GFP
• Species: Mouse

Sequence of Insert

ggatccGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGAATGCCCTGTATTCAAGCTCAATATGGA
ACACCAGCAACGAGCCCAGGACCGCGTGACCACCTGACCGGTGATCCCCT
GGCCCTTGAGTTCGGCAAGCCTACCATGGACCTGGCCAGCCCCGAGACAG
CACCTACCGCACCTGCTACACTGCCCAGCTTCAGCACCTTCATGGACGGG
TACACCGGAGAGTTTGACACCTTCCTCTACCAGCTGCCGGGGACGACCCA
GCCGTGCTCCTCAGCTTGTTCCTCTGCCTCCTCCACGTCTTCTTCCTCAT
CCTCGGCCACCTCCCCCGCTTCGGCGTCCTTCAAGTTTGAGGACTTCCAG
GTGTACGGCTGCTACCCGGGCACCCTGAGCGGCCCATTAGATGAGACCCT
ATCCTCCAGCGGCTCTGAGTACTATGGCAGTCCCTGCTCAGCCCCCTCGC
CATCTACACCCAACTTCCAGCCGTCCCAGCTTTCTCCCTGGGACGGCTCA
TTTGGCCACTTCTCCCCGAGCCAGACTTATGAAGGCCTCTGGGCATGGAC
AGAGCAGTTGCCTAAGGCTTCTTCAGGGCCCCCGCCACCTCCAACCTTCT
TCTCCTTCAGTCCTCCCACTGGCCCCAGCCCCAGCCTGGCCCAGAGTTCT
CTGAAATTGTTCCCACCACCAGCCACCCACCAGCTTGGGGAGGGGGAGAG
CTATTCCATGCCAGCAGCTTTCCCCGGCTTGGCACCCACCTCTCCGAACC
GTGACACTTCCGGCATTCTGGACGCACCCGTGACCTCCACCAAGTCCCGG
AGCGGGGCTTCAGGTGGCAGCGAGGGCCGCTGTGCAGTCTGTGGTGACAA
TGCTTCGTGTCAGCACTATGGGGTCCGCACCTGTGAGGGCTGCAAGGGCT
TCTTCAAGCGCACAGTACAGAAAAGCGCCAAGTACATCTGCCTGGCAAAC
AAGGATTGCCCTGTGGACAAGAGGCGGCGGAACCGCTGCCAGTTCTGCCG
CTTCCAGAAGTGCCTGGCTGTGGGCATGGTGAAGGAAGTTGTACGGACAG
ACAGCCTAAAAGGGCGGCGGGGCCGGCTACCTGACAAACCCAAGCAGCCT
CCAGATGCCTCCCCTACCAATCTTCTCACTTCCCTCATCCGGGCACACTT
GGACTCCGGGCCTAGCACTGCCAAATTGGACTATTCCAAGTTCCAGGAAC
TGGTGCTGCCCCGCTTCGGGAAGGAAGATGCCGGTGACGTGCAACAATTT
TATGACTTGCTCTCTGGTTCCCTGGACGTTATCCGAAAGTGGGCAGAAAA
AATCCCTGGCTTCATTGAGCTTTGCCCAGGAGACCAAGACCTGTTGCTAG
AGTCTGCCTTCCTGGAACTCTTCATCCTCCGCCTGGCATACCGATCTAAA
CCCGGTGAGGGGAAGCTCATCTTCTGCTCAGGCCTGGTACTACACCAGCT
GCAGTGTGCCCGTGGCTTTGGTGATTGGATTGACAACATCCTGGCCTTCT
CACGGTCCCTGCACAGCTTGGGTGTTGATGTCCCCGCCTTTGCCTGCCTG
TCCGCTCTGGTCCTCATCACTGATCGACACGGGCTCCAGGACCCTCGTCG
GGTGGAAGAGCTGCAGAATCGCATTGCTAGCTGTCTGAAGGAGCACATGG
CTACCGTGGCAGGGGACCCACAGCCGGCCAGCTGCCTGTCACGTCTGCTG
GGCAAACTGCCTGAGCTTCGGACCCTGTGCACTCAAGGCCTGCAGCGCAT
CTTTTGCCTCAAGTTGGAGGACTTGGTACCCCCTCCACCTATTGTGGACA
AGATCTTTATGGACACATTGTCTTTCTGAgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGMPCIQAQYG
TPATSPGPRDHLTGDPLALEFGKPTMDLASPETAPTAPATLPSFSTFMDG
YTGEFDTFLYQLPGTTQPCSSACSSASSTSSSSSSATSPASASFKFEDFQ
VYGCYPGTLSGPLDETLSSSGSEYYGSPCSAPSPSTPNFQPSQLSPWDGS
FGHFSPSQTYEGLWAWTEQLPKASSGPPPPPTFFSFSPPTGPSPSLAQSS
LKLFPPPATHQLGEGESYSMPAAFPGLAPTSPNRDTSGILDAPVTSTKSR
SGASGGSEGRCAVCGDNASCQHYGVRTCEGCKGFFKRTVQKSAKYICLAN
KDCPVDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRGRLPDKPKQP
PDASPTNLLTSLIRAHLDSGPSTAKLDYSKFQELVLPRFGKEDAGDVQQF
YDLLSGSLDVIRKWAEKIPGFIELCPGDQDLLLESAFLELFILRLAYRSK
PGEGKLIFCSGLVLHQLQCARGFGDWIDNILAFSRSLHSLGVDVPAFACL
SALVLITDRHGLQDPRRVEELQNRIASCLKEHMATVAGDPQPASCLSRLL
GKLPELRTLCTQGLQRIFCLKLEDLVPPPPIVDKIFMDTLSF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5400
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Tetracycline inducible mammalian expression vector for expression of GFP-Nurr77 S354D.; N.B. Sequence differences compared with X60132 CDS:; g363a (silent P121), t558c (silent P186), t1509c (silent V503), a1641g (silent G547); plus: tca1060-1062gac (S354D)