cDNA Clone Nor1 (mouse)

• Plasmid: pcDNA4/TO GFP.Nor1
• Parent Plasmid: pcDNA4/TO
• Genbank: NM_015743
• Tag: GFP
• Species: Mouse

Sequence of Insert

ggatccGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGAATGCCCTGCGTGCAAGCCCAGTATAGC
CCTTCACCTCCGGGGTCCACTTACGCCACGCAGACTTATGGCTCGGAATA
CACCACAGAAATCATGAACCCCGACTACACCAAGCTGACCATGGACCTCG
GTAGCACGGGGATCATGGCCACTGCCACTACATCCCTGCCCAGCTTCAGT
ACCTTCATGGAGGGCTACCCCAGCAGCTGCGAACTCAAGCCCTCCTGCCT
GTACCAAATGCCGCCTTCTGGGCCTCGGCCTTTGATCAAGATGGAAGAGG
GTCGCGAGCATGGCTACCACCACCACCATCACCATCACCATCATCACCAC
CACCACCAGCAACAGCAGCCGTCCATTCCTCCTCCCTCCGGCCCCGAGGA
CGAGGTACTGCCCAGCACCTCCATGTACTTCAAGCAGTCTCCGCCTTCTA
CACCGACCACTCCAGGCTTCCCCCCGCAGGCGGGGGCGCTGTGGGACGAC
GAGCTGCCCTCTGCGCCTGGCTGCATCGCTCCGGGACCGCTGCTGGACCC
GCAGATGAAGGCGGTACCCCCCATGGCCGCTGCTGCGCGCTTCCCGATCT
TCTTCAAGCCCTCACCGCCACACCCTCCCGCGCCCAGTCCAGCCGGCGGC
CACCACCTCGGCTATGACCCCACGGCCGCAGCTGCACTCAGTCTGCCCCT
GGGAGCCGCGGCCGCAGCAGGCAGCCAAGCTGCTGCGCTCGAGGGCCACC
CATACGGGCTCCCGCTGGCCAAGAGGACGGCCACGCTGACCTTCCCTCCG
CTGGGCCTCACAGCCTCCCCCACCGCGTCCAGCCTGCTGGGAGAGAGCCC
CAGCCTCCCATCGCCACCCAATAGGAGCTCATCATCTGGGGAAGGCACAT
GTGCCGTGTGCGGCGACAACGCTGCCTGCCAGCACTACGGAGTCCGCACC
TGCGAGGGCTGCAAGGGCTTCTTCAAGAGAACGGTGCAGAAAAATGCAAA
ATATGTTTGCCTGGCAAATAAAAACTGCCCAGTGGACAAGAGACGCCGAA
ACCGATGTCAGTACTGCAGATTTCAGAAGTGTCTCAGTGTCGGGATGGTT
AAGGAAGTTGTGCGTACAGACAGTCTGAAAGGGAGGAGAGGTCGTCTGCC
TTCCAAACCAAAGAGCCCACTACAACAGGAGCCCTCGCAGCCCTCCCCGC
CATCTCCTCCGATCTGTATGATGAATGCCCTTGTCCGAGCTTTAACAGAT
GCAACACCCAGAGATCTTGATTATTCCAGATACTGTCCCACCGACCAGGC
CACTGCAGGCACAGATGCTGAGCACGTGCAACAGTTCTACAACCTTCTGA
CGGCCTCCATTGACGTGTCCAGAAGCTGGGCAGAAAAGATCCCAGGATTC
ACTGATCTCCCCAAAGAAGATCAGACGTTACTTATAGAATCAGCCTTTTT
GGAGCTGTTTGTTCTTAGACTTTCCATCAGGTCAAACACTGCTGAAGATA
AGTTTGTGTTCTGCAATGGACTTGTCCTGCATCGACTTCAGTGCCTTCGA
GGATTTGGGGAATGGCTCGACTCCATTAAAGACTTTTCTTTAAACTTGCA
GAGCCTGAACCTTGATATCCAAGCCTTAGCCTGCCTGTCAGCACTGAGTA
TGATCACAGAGCGACATGGGTTAAAAGAACCAAAGAGAGTGGAGGAGCTA
TGCACCAAGATCACAAGCAGCTTAAAGGACCACCAGAGGAAGGGACAGGC
TCTGGAGCCCTCGGAGCCTAAGGTCCTGCGCGCGCTGGTAGAACTGAGAA
AGATCTGTACCCAGGGCCTCCAGCGCATCTTCTACCTGAAGCTAGAGGAC
TTGGTACCTCCGCCTTCTGTCATCGACAAGCTCTTCCTTGACACCCTGCC
TTTCTGAgaattc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGMPCVQAQYS
PSPPGSTYATQTYGSEYTTEIMNPDYTKLTMDLGSTGIMATATTSLPSFS
TFMEGYPSSCELKPSCLYQMPPSGPRPLIKMEEGREHGYHHHHHHHHHHH
HHQQQQPSIPPPSGPEDEVLPSTSMYFKQSPPSTPTTPGFPPQAGALWDD
ELPSAPGCIAPGPLLDPQMKAVPPMAAAARFPIFFKPSPPHPPAPSPAGG
HHLGYDPTAAAALSLPLGAAAAAGSQAAALEGHPYGLPLAKRTATLTFPP
LGLTASPTASSLLGESPSLPSPPNRSSSSGEGTCAVCGDNAACQHYGVRT
CEGCKGFFKRTVQKNAKYVCLANKNCPVDKRRRNRCQYCRFQKCLSVGMV
KEVVRTDSLKGRRGRLPSKPKSPLQQEPSQPSPPSPPICMMNALVRALTD
ATPRDLDYSRYCPTDQATAGTDAEHVQQFYNLLTASIDVSRSWAEKIPGF
TDLPKEDQTLLIESAFLELFVLRLSIRSNTAEDKFVFCNGLVLHRLQCLR
GFGEWLDSIKDFSLNLQSLNLDIQALACLSALSMITERHGLKEPKRVEEL
CTKITSSLKDHQRKGQALEPSEPKVLRALVELRKICTQGLQRIFYLKLED
LVPPPSVIDKLFLDTLPF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5388
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Tetracycline inducible mammalian expression vector for expression of Nor1 with N-terminal GFP.; N.B. Sequence differences compared with NM_015743 CDS:; c150t (silent T50), g423t (silent P141), g1539a (silent E513), a1839g (silent P613); (All derive from Nor1 cloned by Mark Peggie)