cDNA Clone mcm3

• Plasmid: pEBG6P-1 mcm3
• Parent Plasmid: pEBG6P
• Expressed: GST-mcm3
• Genbank: NM_002388
• Tag: GST

Sequence of Insert

gcggccgcgATGGCGGGTACCGTGGTGCTGGACGATGTGGAGCTGCGGGAGGCTCAGAGAGATTACCTGGACTTCCTGGACGACGAGGAAGACCAGGGAATTTATCAGAGCAAAGTTCGGGAGCTGATCAGTGACAACCAATACCGGCTGATTGTCAATGTGAATGACCTGCGCAGGAAAAACGAGAAGAGGGCTAACCGGCTTCTGAACAATGCCTTTGAGGAGCTGGTTGCCTTCCAGCGGGCCTTAAAGGATTTTGTGGCCTCCATTGATGCTACCTATGCCAAGCAGTATGAGGAGTTCTACGTAGGACTGGAAGGCAGCTTTGGCTCCAAGCACGTCTCCCCGCGGACTCTTACCTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAGGGCATTGTCACTAAATGTTCTCTAGTTCGTCCCAAAGTCGTCCGCAGTGTCCACTACTGTCCTGCT
ACTAAGAAGACCATAGAGCGACGTTATTCTGATCTCACCACCCTGGTGGC
CTTTCCCTCCAGCTCTGTCTATCCTACCAAGGATGAGGAGAACAATCCCC
TTGAGACAGAATATGGCCTTTCTGTCTACAAGGATCACCAGACCATCACC
ATCCAGGAGATGCCGGAGAAGGCCCCAGCCGGCCAGCTCCCCCGCTCTGT
GGACGTCATTCTGGATGATGACTTGGTGGATAAAGCGAAGCCTGGTGACC
GGGTTCAGGTGGTGGGAACCTACCGTTGCCTTCCTGGAAAGAAGGGAGGC
TACACCTCTGGGACCTTCAGGACTGTCCTGATTGCCTGTAATGTTAAGCA
GATGAGCAAGGATGCTCAGCCCTCTTTCTCTGCTGAGGATATAGCCAAGA
TCAAGAAGTTCAGTAAAACCCGATCCAAGGATATCTTTGACCAGCTGGCC
AAGTCATTGGCCCCAAGTATCCATGGGCATGACTATGTCAAGAAAGCAAT
CCTCTGCTTGCTCTTGGGAGGGGTGGAACGAGACCTAGAAAATGGCAGCC
ACATCCGTGGGGACATCAATATTCTTCTAATAGGAGACCCATCCGTTGCC
AAGTCTCAGCTTCTGCGGTATGTGCTTTGCACTGCACCCCGAGCTATCCC
CACCACTGGCCGGGGCTCCTCTGGAGTGGGTCTGACGGCTGCTGTCACCA
CAGACCAGGAAACAGGAGAGCGCCGTCTGGAAGCAGGGGCCATGGTCCTG
GCTGACCGAGGCGTGGTTTGCATTGATGAATTTGACAAAATGTCTGACAT
GGATCGCACAGCCATCCATGAAGTGATGGAGCAGGGTCGAGTGACCATTG
CCAAGGCTGGCATCCATGCTCGGCTGAATGCCCGCTGCAGTGTTTTGGCA
GCTGCCAACCCTGTCTACGGCAGGTATGACCAGTATAAGACTCCAATGGA
GAACATTGGGCTACAGGACTCACTGCTGTCACGATTTGACTTGCTCTTCA
TCATGCTGGATCAGATGGATCCTGAGCAGGATCGGGAGATCTCAGACCAT
GTCCTTCGGATGCACCGTTACAGAGCACCTGGGGAGCAGGATGGCGATGC
TATGCCCTTGGGTAGTGCTGTGGATATCCTGGCCACAGATGATCCCAACT
TTAGCCAGGAAGATCAGCAGGACACCCAGATTTATGAGAAGCATGACAAC
CTTCTACATGGGACCAAGAAGAAAAAGGAGAAGATGGTGAGTGCAGCATT
CATGAAGAAGTACATCCATGTGGCCAAAATCATCAAGCCTGTCCTGACAC
AGGAGTCGGCCACCTACATTGCAGAAGAGTATTCACGCCTGCGCAGCCAG
GATAGCATGAGCTCAGACACCGCCAGGACATCTCCAGTTACAGCCCGAAC
ACTGGAAACTCTGATTCGACTGGCCACAGCCCATGCGAAGGCCCGCATGA
GCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCTGTGGAGTTGGTCCAG
TATGCTTACTTTAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGCG
AAGTGAGGATGAATCAGAGACAGAAGATGAAGAGGAGAAAAGCCAAGAGG
ACCAGGAGCAGAAGAGGAAGAGAAGGAAGACTCGCCAGCCAGATGCCAAA
GATGGGGATTCATACGACCCCTATGACTTCAGTGACACAGAGGAGGAAAT
GCCTCAAGTACACACTCCAAAGACGGCAGACTCACAGGAGACCAAGGAAT
CCCAGAAAGTGGAGTTGAGTGAATCCAGGTTGAAGGCATTCAAGGTGGCC
CTCTTGGATGTGTTCCGGGAAGCTCATGCGCAGTCAATCGGCATGAATCG
CCTCACAGAATCCATCAACCGGGACAGCGAAGAGCCCTTCTCTTCAGTTG
AGATCCAGGCTGCTCTGAGCAAGATGCAGGATGACAATCAGGTCATGGTG
TCTGAGGGCATCATCTTCCTCATCTGAgcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMAGT
VVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDL
RRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEG
SFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKT
IERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEM
PEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSG
TFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLA
PSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQL
LRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRG
VVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANP
VYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRM
HRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHG
TKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMS
SDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYF
KKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDS
YDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDV
FREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGI
IFLI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5327
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express mcm3 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 119111 Daltons