cDNA Clone mcm3

• Plasmid: pGEX6P-1-mcm3
• Parent Plasmid: pGEX6P1
• Expressed: GST-mcm3
• Genbank: NM_002388
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGGCGGGTACCGTGGTGCTGGACGATGTGGAGCTGCGGGAGGCTCAGAGAGATTACCTGGACTTCCTGGACGACGAGGAAGACCAGGGAATTTATCAGAGCAAAGTTCGGGAGCTGATCAGTGACAACCAATACCGGCTGATTGTCAATGTGAATGACCTGCGCAGGAAAAACGAGAAGAGGGCTAACCGGCTTCTGAACAATGCCTTTGAGGAGCTGGTTGCCTTCCAGCGGGCCTTAAAGGATTTTGTGGCCTCCATTGATGCTACCTATGCCAAGCAGTATGAGGAGTTCTACGTA
GGACTGGAAGGCAGCTTTGGCTCCAAGCACGTCTCCCCGCGGACTCTTAC
CTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAGGGCATTGTCACTAAAT
GTTCTCTAGTTCGTCCCAAAGTCGTCCGCAGTGTCCACTACTGTCCTGCT
ACTAAGAAGACCATAGAGCGACGTTATTCTGATCTCACCACCCTGGTGGC
CTTTCCCTCCAGCTCTGTCTATCCTACCAAGGATGAGGAGAACAATCCCC
TTGAGACAGAATATGGCCTTTCTGTCTACAAGGATCACCAGACCATCACC
ATCCAGGAGATGCCGGAGAAGGCCCCAGCCGGCCAGCTCCCCCGCTCTGT
GGACGTCATTCTGGATGATGACTTGGTGGATAAAGCGAAGCCTGGTGACC
GGGTTCAGGTGGTGGGAACCTACCGTTGCCTTCCTGGAAAGAAGGGAGGC
TACACCTCTGGGACCTTCAGGACTGTCCTGATTGCCTGTAATGTTAAGCA
GATGAGCAAGGATGCTCAGCCCTCTTTCTCTGCTGAGGATATAGCCAAGA
TCAAGAAGTTCAGTAAAACCCGATCCAAGGATATCTTTGACCAGCTGGCC
AAGTCATTGGCCCCAAGTATCCATGGGCATGACTATGTCAAGAAAGCAAT
CCTCTGCTTGCTCTTGGGAGGGGTGGAACGAGACCTAGAAAATGGCAGCC
ACATCCGTGGGGACATCAATATTCTTCTAATAGGAGACCCATCCGTTGCC
AAGTCTCAGCTTCTGCGGTATGTGCTTTGCACTGCACCCCGAGCTATCCC
CACCACTGGCCGGGGCTCCTCTGGAGTGGGTCTGACGGCTGCTGTCACCA
CAGACCAGGAAACAGGAGAGCGCCGTCTGGAAGCAGGGGCCATGGTCCTG
GCTGACCGAGGCGTGGTTTGCATTGATGAATTTGACAAAATGTCTGACAT
GGATCGCACAGCCATCCATGAAGTGATGGAGCAGGGTCGAGTGACCATTG
CCAAGGCTGGCATCCATGCTCGGCTGAATGCCCGCTGCAGTGTTTTGGCA
GCTGCCAACCCTGTCTACGGCAGGTATGACCAGTATAAGACTCCAATGGA
GAACATTGGGCTACAGGACTCACTGCTGTCACGATTTGACTTGCTCTTCA
TCATGCTGGATCAGATGGATCCTGAGCAGGATCGGGAGATCTCAGACCAT
GTCCTTCGGATGCACCGTTACAGAGCACCTGGGGAGCAGGATGGCGATGC
TATGCCCTTGGGTAGTGCTGTGGATATCCTGGCCACAGATGATCCCAACT
TTAGCCAGGAAGATCAGCAGGACACCCAGATTTATGAGAAGCATGACAAC
CTTCTACATGGGACCAAGAAGAAAAAGGAGAAGATGGTGAGTGCAGCATT
CATGAAGAAGTACATCCATGTGGCCAAAATCATCAAGCCTGTCCTGACAC
AGGAGTCGGCCACCTACATTGCAGAAGAGTATTCACGCCTGCGCAGCCAG
GATAGCATGAGCTCAGACACCGCCAGGACATCTCCAGTTACAGCCCGAAC
ACTGGAAACTCTGATTCGACTGGCCACAGCCCATGCGAAGGCCCGCATGA
GCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCTGTGGAGTTGGTCCAG
TATGCTTACTTTAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGCG
AAGTGAGGATGAATCAGAGACAGAAGATGAAGAGGAGAAAAGCCAAGAGG
ACCAGGAGCAGAAGAGGAAGAGAAGGAAGACTCGCCAGCCAGATGCCAAA
GATGGGGATTCATACGACCCCTATGACTTCAGTGACACAGAGGAGGAAAT
GCCTCAAGTACACACTCCAAAGACGGCAGACTCACAGGAGACCAAGGAAT
CCCAGAAAGTGGAGTTGAGTGAATCCAGGTTGAAGGCATTCAAGGTGGCC
CTCTTGGATGTGTTCCGGGAAGCTCATGCGCAGTCAATCGGCATGAATCG
CCTCACAGAATCCATCAACCGGGACAGCGAAGAGCCCTTCTCTTCAGTTG
AGATCCAGGCTGCTCTGAGCAAGATGCAGGATGACAATCAGGTCATGGTG
TCTGAGGGCATCATCTTCCTCATCTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMAGTVVLDDVELREAQ
RDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEKRANRLL
NNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTL
TSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLV
AFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRS
VDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVK
QMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKA
ILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAI
PTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSD
MDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPM
ENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRMHRYRAPGEQDGD
AMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHGTKKKKEKMVSAA
FMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSPVTAR
TLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKK
RSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEE
MPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMN
RLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5286
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 118103 Daltons