cDNA Clone mcm3

• Plasmid: pCMV5-HA mcm3
• Parent Plasmid: pCMV5
• Expressed: HA-mcm3
• Genbank: NM_002388
• Tag: HA
• Species: Human

Sequence of Insert

gaattcGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGCGGGTACCGTGGTGCTGGACGATGTGGAGCTGCGGGAGGCTCAGAGAGATTACCTGGACTTCCTGGACGACGAGGAAGACCAGGGAATTTATCAGAGCAAAGTTCGGGAGCTGATCAGTGACAACCAATACCGGCTGATTGTCAATGTGAATGACCTGCGCAGGAAAAACGAGAAGAGGGCTAACCGGCTTCTGAACAATGCCTTTGAGGAGCTGGTTGCCTTCCAGCGGGCCTTAAAGGATTTTGTGGCCTCCATTGATGCTACCTATGCCAAGCAGTATGAGGAGTTCTACGTAGGACTGGAAGGCAGCTTTGGCTCCAAGCACGTCTCCCCGCGGACTCTTACCTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAGGGCATTGTCACTAAATGTTCTCTAGTTCGTCCCAAAGTCGTCCGCAGTGTCCACTACTGTCCTGCTACTAAGAAGACCATAGAGCGACGTTATTCTGATCTCACCACCCTGGTGGCCTTTCCCTCCAGCTCTGTCTATCCTACCAAGGATGAGGAGAACAATCCCCTTGAGACAGAATATGGCCTTTCTGTCTACAAGGATCACCAGACCATCACCATCCAGGAGATGCCGGAGAAGGCCCCAGCCGGCCAGCTCCCCCGCTCTGTGGACGTCATTCTGGATGATGACTTGGTGGATAAAGCGAAGCCTGGTGACCGGGTTCAGGTGGTGGGAACCTACCGTTGCCTTCCTGGAAAGAAGGGAGGCTACACCTCTGGGACCTTCAGGACTGTCCTGATTGCCTGTAATGTTAAGCAGATGAGCAAGGATGCTCAGCCCTCTTTCTCTGCTGAGGATATAGCCAAGATCAAGAAGTTCAGTAAAACCCGATCCAAGGATATCTTTGACCAGCTGGCCAAGTCATTGGCCCCAAGTATCCATGGGCATGACTATGTCAAGAAAGCAATCCTCTGCTTGCTCTTGGGAGGGGTGGAACGAGACCTAGAAAATGGCAGCCACATCCGTGGGGACATCAATATTCTTCTAATAGGAGACCCATCCGTTGCCAAGTCTCAGCTTCTGCGGTATGTGCTTTGCACTGCACCCCGAGCTATCCCCACCACTGGCCGGGGCTCCTCTGGAGTGGGTCTGACGGCTGCTGTCACCACAGACCAGGAAACAGGAGAGCGCCGTCTGGAAGCAGGGGCCATGGTCCTGGCTGACCGAGGCGTGGTTTGCATTGATGAATTTGACAAAATGTCTGACATGGATCGCACAGCCATCCATGAAGTGATGGAGCAGGGTCGAGTGACCATTGCCAAGGCTGGCATCCATGCTCGGCTGAATGCCCGCTGCAGTGTTTTGGCAGCTGCCAACCCTGTCTACGGCAGGTATGACCAGTATAAGACTCCAATGGAGAACATTGGGCTACAGGACTCACTGCTGTCACGATTTGACTTGCTCTTCATCATGCTGGATCAGATGGATCCTGAGCAGGATCGGGAGATCTCAGACCATGTCCTTCGGATGCACCGTTACAGAGCACCTGGGGAGCAGGATGGCGATGCTATGCCCTTGGGTAGTGCTGTGGATATCCTGGCCACAGATGATCCCAACTTTAGCCAGGAAGATCAGCAGGACACCCAGATTTATGAGAAGCATGACAACCTTCTACATGGGACCAAGAAGAAAAAGGAGAAGATGGTGAGTGCAGCATTCATGAAGAAGTACATCCATGTGGCCAAAATCATCAAGCCTGTCCTGACACAGGAGTCGGCCACCTACATTGCAGAAGAGTATTCACGCCTGCGCAGCCAGGATAGCATGAGCTCAGACACCGCCAGGACATCTCCAGTTACAGCCCGAACACTGGAAACTCTGATTCGACTGGCCACAGCCCATGCGAAGGCCCGCATGAGCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCTGTGGAGTTGGTCCAGTATGCTTACTTTAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGCGAAGTGAGGATGAATCAGAGACAGAAGATGAAGAGGAGAAAAGCCAAGAGGACCAGGAGCAGAAGAGGAAGAGAAGGAAGACTCGCCAGCCAGATGCCAAA
GATGGGGATTCATACGACCCCTATGACTTCAGTGACACAGAGGAGGAAAT
GCCTCAAGTACACACTCCAAAGACGGCAGACTCACAGGAGACCAAGGAAT
CCCAGAAAGTGGAGTTGAGTGAATCCAGGTTGAAGGCATTCAAGGTGGCC
CTCTTGGATGTGTTCCGGGAAGCTCATGCGCAGTCAATCGGCATGAATCG
CCTCACAGAATCCATCAACCGGGACAGCGAAGAGCCCTTCTCTTCAGTTG
AGATCCAGGCTGCTCTGAGCAAGATGCAGGATGACAATCAGGTCATGGTG
TCTGAGGGCATCATCTTCCTCATCTGAgtcgac

Amino Acid Sequence

MYPYDVPDYAMAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELI
SDNQYRLIVNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDAT
YAKQYEEFYVGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPK
VVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGL
SVYKDHQTITIQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGT
YRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKT
RSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDIN
ILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGE
RRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHA
RLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMD
PEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQ
DTQIYEKHDNLLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYI
AEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQ
DAEEAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRK
RRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELS
ESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALS
KMQDDNQVMVSEGIIFLI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5285
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 92138 Daltons