cDNA Clone DNA-PK

• Plasmid: pFastBAc.HTa DNA-PK CAT
• Parent Plasmid: pSC-A
• Expressed: 6His-DNA-PK CAT
• Genbank: NP_008835
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcAATAAAAAAAACATTGAAAAAATGTATGAAAGAATGTATGCAGCCTTGGGTGACCCAAAGGCTCCAGGCCTGGGGGCCTTTAGAAGGAAGTTTATTCAGACTTTTGGAAAAGAATTTGATAAACATTTTGGGAAAGGAGGTTCTAAACTACTGAGAATGAAGCTCAGTGACTTCAACGACATTACCAACATGCTACTTTTAAAAATGAACAAAGACTCAAAGCCCCCTGGGAATCTGAAAGAATGTTCACCCTGGATGAGCGACTTCAAAGTGGAGTTCCTGAGAAATGAGCTGGAGATT
CCCGGTCAGTATGACGGTAGGGGAAAGCCATTGCCAGAGTACCACGTGCG
AATCGCCGGGTTTGATGAGCGGGTGACAGTCATGGCGTCTCTGCGAAGGC
CCAAGCGCATCATCATCCGTGGCCATGACGAGAGGGAACACCCTTTCCTG
GTGAAGGGTGGCGAGGACCTGCGGCAGGACCAGCGCGTGGAGCAGCTCTT
CCAGGTCATGAATGGGATCCTGGCCCAAGACTCCGCCTGCAGCCAGAGGG
CCCTGCAGCTGAGGACCTATAGCGTTGTGCCCATGACCTCCAGGTTAGGA
TTAATTGAGTGGCTTGAAAATACTGTTACCTTGAAGGACCTTCTTTTGAA
CACCATGTCCCAAGAGGAGAAGGCGGCTTACCTGAGTGATCCCAGGGCAC
CGCCGTGTGAATATAAAGATTGGCTGACAAAAATGTCAGGAAAACATGAT
GTTGGAGCTTACATGCTAATGTATAAGGGCGCTAATCGTACTGAAACAGT
CACGTCTTTTAGAAAACGAGAAAGTAAAGTGCCTGCTGATCTCTTAAAGC
GGGCCTTCGTGAGGATGAGTACAAGCCCTGAGGCTTTCCTGGCGCTCCGC
TCCCACTTCGCCAGCTCTCACGCTCTGATATGCATCAGCCACTGGATCCT
CGGGATTGGAGACAGACATCTGAACAACTTTATGGTGGCCATGGAGACTG
GCGGCGTGATCGGGATCGACTTTGGGCATGCGTTTGGATCCGCTACACAG
TTTCTGCCAGTCCCTGAGTTGATGCCTTTTCGGCTAACTCGCCAGTTTAT
CAATCTGATGTTACCAATGAAAGAAACGGGCCTTATGTACAGCATCATGG
TACACGCACTCCGGGCCTTCCGCTCAGACCCTGGCCTGCTCACCAACACC
ATGGATGTGTTTGTCAAGGAGCCCTCCTTTGATTGGAAAAATTTTGAACA
GAAAATGCTGAAAAAAGGAGGGTCATGGATTCAAGAAATAAATGTTGCTG
AAAAAAATTGGTACCCCCGACAGAAAATATGTTACGCTAAGAGAAAGTTA
GCAGGTGCCAATCCAGCAGTCATTACTTGTGATGAGCTACTCCTGGGTCA
TGAGAAGGCCCCTGCCTTCAGAGACTATGTGGCTGTGGCACGAGGAAGCA
AAGATCACAACATTCGTGCCCAAGAACCAGAGAGTGGGCTTTCAGAAGAG
ACTCAAGTGAAGTGCCTGATGGACCAGGCAACAGACCCCAACATCCTTGG
CAGAACCTGGGAAGGATGGGAGCCCTGGATGTGAgaattc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFNKKNIEKMYERMYAALGDPK
APGLGAFRRKFIQTFGKEFDKHFGKGGSKLLRMKLSDFNDITNMLLLKMN
KDSKPPGNLKECSPWMSDFKVEFLRNELEIPGQYDGRGKPLPEYHVRIAG
FDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQRVEQLFQVM
NGILAQDSACSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLLNTMS
QEEKAAYLSDPRAPPCEYKDWLTKMSGKHDVGAYMLMYKGANRTETVTSF
RKRESKVPADLLKRAFVRMSTSPEAFLALRSHFASSHALICISHWILGIG
DRHLNNFMVAMETGGVIGIDFGHAFGSATQFLPVPELMPFRLTRQFINLM
LPMKETGLMYSIMVHALRAFRSDPGLLTNTMDVFVKEPSFDWKNFEQKML
KKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKA
PAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTW
EGWEPWM*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5271
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 63926 Daltons