cDNA Clone F2KP (Arabidopsis)

• Plasmid: pEBG6P-1 At F2KP S220A
• Parent Plasmid: pEBG6P
• Genbank: AF190739
• Species: Arabidopsis thaliana

Sequence of Insert

ggtaccATGGGGTCAGGTGCATCGAAGAATACTGAGGAAGATGACGATGGTTCCAATGGCGGAGGTGGTCAGCTCTACGTTTCACTCAAAATGGAAAACTCTAAGGTTGAAGGAGAGCTCACTCCTCATGTCTATGGCTCTCTTCCTCTCATCGGTTCCTGGGATCCTTCCAAAGCTCTTCCGATGCAACGTGAATCTGCGTTAATGTCGGAGCTAAGCTTCGTTGTTCCTCCTGATCACGAGACTTTGGACTTCAAGTTTTTGTTGAAGCCAAAGAACAGAAATACGCCCTGCATAGTGGAGGAG
GGTGAAAATAGGCTTCTTACTGGTGGTTCGTTGCAAGGAGATGCTAGATT
AGCTCTGTTTAGGCTGGAAGGTGATGTGATTGTTGAATTCCGAGTCTTCA
TCAACGCTGACAGAGTTTCTCCAATTGATCTTGCTACTAGTTGGAGAGCT
TATAGGGAGAACCTCCAGCCTTCTACTGTACGTGGAATTCCTGATGTCAG
CATTAATCCTGATCCAAAATCTGCTGAGTGTCCTTTAGAAAGTTTGGAGC
TCGATCTTGCACATTATGAAGTTCCTGCTCCTGCGCCTTCTGCAAACTCG
TACCTTGTTTATGCGGCGGACAATGCAGAGAATCCACGATCTCTTTCAGC
TTCTGGTGCATTTCGTAATGACAGTACTCCAAAAGCAGCACAGAGAAATT
CTGAAGATTCTGGTGTTACGGTTGATGGATCCCCATCTGCAAAGGAAATG
ACGATCGTTGTTCCTGATTCCTCGAATATCTATTCGGCTTTTGGAGAGGC
TGAGTCAAAGTCTGTGGAGACACTCTCACCGTTTCAGCAGAAAGATGGTC
AAAAGGGACTCTTTGTTGATCGTGGTGTTGGCTCCCCTAGGCTAGTGAAG
TCTCTTAGTGCAAGCTCTTTTTTGATTGATACCAAGCAAATCAAGAATTC
AATGCCAGCCGCTGCAGGAGCTGTTGCCGCAGCAGCGGTAGCTGATCAAA
TGCTTGGACCGAAAGAAGATAGACATCTAGCAATTGTCCTGGTTGGCTTG
CCTGCTCGAGGAAAGACTTTTACTGCAGCAAAGTTGACAAGATATCTACG
CTGGTTGGGACATGATACTAAACATTTTAATGTTGGAAAGTACCGACGGC
TTAAACATGGAGTTAACATGAGTGCTGATTTCTTCCGAGCTGATAATCCT
GAAGGTGTAGAGGCACGGACTGAGGTAGCAGCACTTGCTATGGAGGACAT
GATAGCTTGGATGCAAGAAGGTGGTCAAGTTGGAATCTTTGATGCAACTA
ATAGCACACGAGTTCGACGGAATATGTTGATGAAAATGGCTGAAGGAAAG
TGTAAGATCATTTTTCTGGAAACACTATGCAATGATGAGCGCATTATTGA
AAGAAACATACGTCTAAAAATCCAGCAAAGTCCTGACTATTCAGAAGAAA
TGGATTTTGAAGCTGGAGTACGAGACTTTAGAGACCGTTTAGCCAATTAT
GAAAAGGTTTATGAGCCTGTAGAAGAAGGTTCGTACATCAAAATGATTGA
TATGGTCAGTGGGAACGGTGGACAGATACAAGTGAACAATATTAGTGGTT
ACCTTCCTGGGAGAATTGTATTTTTCTTGGTGAATACGCATCTCACCCCA
CGACCAATACTACTCACTCGGCATGGAGAAAGCATGGATAATGTTAGAGG
CAGAATTGGAGGAGATAGCGTAATAAGTGACTCTGGTAAACTTTACGCAA
AGAAACTTGCCAGCTTTGTTGAAAAGCGACTTAAATCTGAAAAAGCTGCT
TCGATTTGGACCAGTACACTTCAGAGAACAAACCTAACAGCAAGTTCCAT
TGTCGGATTCCCCAAGGTGCAATGGCGTGCCCTGGACGAGATCAATGCTG
GAGTTTGTGATGGGATGACATATGAAGAGGTAAAGAAGAACATGCCAGAA
GAATACGAATCTCGAAAAAAGGACAAATTACGATACCGATACCCTCGTGG
AGAGTCCTACCTTGATGTAATCCAACGGCTTGAACCTGTGATCATTGAGT
TAGAACGACAAAGAGCTCCTGTCGTCGTGATATCTCACCAGGCTGTTCTG
AGAGCCTTGTATGCATATTTTGCGGATCGTCCCCTCAAAGAAATACCCCA
AATCGAGATGCCACTTCACACTATAATAGAGATACAGATGGGAGTTTCTG
GTGTGCAAGAGAAACGCTACAAACTCATGGACTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTMGSGASKNT
EEDDDGSNGGGGQLYVSLKMENSKVEGELTPHVYGSLPLIGSWDPSKALP
MQRESALMSELSFVVPPDHETLDFKFLLKPKNRNTPCIVEEGENRLLTGG
SLQGDARLALFRLEGDVIVEFRVFINADRVSPIDLATSWRAYRENLQPST
VRGIPDVSINPDPKSAECPLESLELDLAHYEVPAPAPSANSYLVYAADNA
ENPRSLSASGAFRNDSTPKAAQRNSEDSGVTVDGSPSAKEMTIVVPDSSN
IYSAFGEAESKSVETLSPFQQKDGQKGLFVDRGVGSPRLVKSLSASSFLI
DTKQIKNSMPAAAGAVAAAAVADQMLGPKEDRHLAIVLVGLPARGKTFTA
AKLTRYLRWLGHDTKHFNVGKYRRLKHGVNMSADFFRADNPEGVEARTEV
AALAMEDMIAWMQEGGQVGIFDATNSTRVRRNMLMKMAEGKCKIIFLETL
CNDERIIERNIRLKIQQSPDYSEEMDFEAGVRDFRDRLANYEKVYEPVEE
GSYIKMIDMVSGNGGQIQVNNISGYLPGRIVFFLVNTHLTPRPILLTRHG
ESMDNVRGRIGGDSVISDSGKLYAKKLASFVEKRLKSEKAASIWTSTLQR
TNLTASSIVGFPKVQWRALDEINAGVCDGMTYEEVKKNMPEEYESRKKDK
LRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFAD
RPLKEIPQIEMPLHTIIEIQMGVSGVQEKRYKLMD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5235
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express F2KP S220A with N-terminal GST (contains precission protease site); N.B. Sequence differences compared with AF190739 CDS: ; t658g (S220A); c1886a (A629D - present in several other mRNAs); ; Estimated MW of expressed protein : 110253 Daltons