cDNA Clone F2KP (Arabidopsis)

• Plasmid: pEBG6P-1 At F2KP
• Parent Plasmid: pEBG6P
• Genbank: AF190739
• Species: Arabidopsis thaliana

Sequence of Insert

ggtaccATGGGGTCAGGTGCATCGAAGAATACTGAGGAAGATGACGATGGTTCCAATGGCGGAGGTGGTCAGCTCTACGTTTCACTCAAAATGGAAAACTCTAAGGTTGAAGGAGAGCTCACTCCTCATGTCTATGGCTCTCTTCCTCTCATCGGTTCCTGGGATCCTTCCAAAGCTCTTCCGATGCAACGTGAATCTGCGTTAATGTCGGAGCTAAGCTTCGTTGTTCCTCCTGATCACGAGACTTTGGACTTCAAGTTTTTGTTGAAGCCAAAGAACAGAAATACGCCCTGCATAGTGGAGGAG
GGTGAAAATAGGCTTCTTACTGGTGGTTCGTTGCAAGGAGATGCTAGATT
AGCTCTGTTTAGGCTGGAAGGTGATGTGATTGTTGAATTCCGAGTCTTCA
TCAACGCTGACAGAGTTTCTCCAATTGATCTTGCTACTAGTTGGAGAGCT
TATAGGGAGAACCTCCAGCCTTCTACTGTACGTGGAATTCCTGATGTCAG
CATTAATCCTGATCCGAAATCTGCTGAGTGTCCTTTAGAAAGTTTGGAGC
TCGATCTTGCACATTATGAAGTTCCTGCTCCTGCGCCTTCTGCAAACTCG
TACCTTGTTTATGCGGCGGACAATGCAGAGAATCCACGATCTCTTTCAGC
TTCTGGTTCATTTCGTAATGACAGTACTCCAAAAGCAGCACAGAGAAATT
CTGAAGATTCTGGTGTTACGGTTGATGGATCCCCATCTGCAAAGGAAATG
ACGATCGTTGTTCCTGATTCCTCGAATATCTATTCGGCTTTTGGAGAGGC
TGAGTCAAAGTCTGTGGAGACACTCTCACCGTTTCAGCAGAAAGATGGTC
AAAAGGGACTCTTTGTTGATCGTGGTGTTGGCTCCCCTAGGCTAGTGAAG
TCTCTTAGTGCAAGCTCTTTTTTGATTGATACCAAGCAAATCAAGAATTC
AATGCCAGCCGCTGCAGGAGCTGTTGCCGCAGCAGCGGTAGCTGATCAAA
TGCTTGGACCGAAAGAAGATAGACATCTAGCAATTGTCCTGGTTGGCTTG
CCTGCTCGAGGAAAGACTTTTACTGCAGCAAAGTTGACAAGATATCTACG
CTGGTTGGGACATGATACTAAACATTTTAATGTTGGAAAGTACCGACGGC
TTAAACATGGAGTTAACATGAGTGCTGATTTCTTCCGAGCTGATAATCCT
GAAGGTGTAGAGGCACGGACTGAGGTAGCAGCACTTGCTATGGAGGACAT
GATAGCTTGGATGCAAGAAGGTGGTCAAGTTGGAATCTTTGATGCAACTA
ATAGCACACGAGTTCGACGGAATATGTTGATGAAAATGGCTGAAGGAAAG
TGTAAGATCATTTTTCTGGAAACACTATGCAATGATGAGCGCATTATTGA
AAGAAACATACGTCTAAAAATCCAGCAAAGTCCTGACTATTCAGAAGAAA
TGGATTTTGAAGCTGGAGTACGAGACTTTAGAGACCGTTTAGCCAATTAT
GAAAAGGTTTATGAGCCTGTAGAAGAAGGTTCGTACATCAAAATGATTGA
TATGGTCAGTGGGAACGGTGGACAGATACAAGTGAACAATATTAGTGGTT
ACCTTCCTGGGAGAATTGTATTTTTCTTGGTGAATACGCATCTCACCCCA
CGACCAATACTACTCACTCGGCATGGAGAAAGCATGGATAATGTTAGAGG
CAGAATTGGAGGAGATAGCGTAATAAGTGACTCTGGTAAACTTTACGCAA
AGAAACTTGCCAGCTTTGTTGAAAAGCGACTTAAATCTGAAAAAGCTGCT
TCGATTTGGACCAGTACACTTCAGAGAACAAACCTAACAGCAAGTTCCAT
TGTCGGATTCCCCAAGGTGCAATGGCGTGCCCTGGACGAGATCAATGCTG
GAGTTTGTGATGGGATGACATATGAAGAGGTAAAGAAGAACATGCCAGAA
GAATACGAATCTCGAAAAAAGGACAAATTACGATACCGATACCCTCGTGG
AGAGTCCTACCTTGATGTAATCCAACGGCTTGAACCTGTGATCATTGAGT
TAGAACGACAAAGAGCTCCTGTCGTCGTGATATCTCACCAGGCTGTTCTG
AGAGCCTTGTATGCATATTTTGCGGATCGTCCCCTCAAAGAAATACCCCA
AATCGAGATGCCACTTCACACTATAATAGAGATACAGATGGGAGTTTCTG
GTGTGCAAGAGAAACGCTACAAACTCATGGACTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTMGSGASKNT
EEDDDGSNGGGGQLYVSLKMENSKVEGELTPHVYGSLPLIGSWDPSKALP
MQRESALMSELSFVVPPDHETLDFKFLLKPKNRNTPCIVEEGENRLLTGG
SLQGDARLALFRLEGDVIVEFRVFINADRVSPIDLATSWRAYRENLQPST
VRGIPDVSINPDPKSAECPLESLELDLAHYEVPAPAPSANSYLVYAADNA
ENPRSLSASGSFRNDSTPKAAQRNSEDSGVTVDGSPSAKEMTIVVPDSSN
IYSAFGEAESKSVETLSPFQQKDGQKGLFVDRGVGSPRLVKSLSASSFLI
DTKQIKNSMPAAAGAVAAAAVADQMLGPKEDRHLAIVLVGLPARGKTFTA
AKLTRYLRWLGHDTKHFNVGKYRRLKHGVNMSADFFRADNPEGVEARTEV
AALAMEDMIAWMQEGGQVGIFDATNSTRVRRNMLMKMAEGKCKIIFLETL
CNDERIIERNIRLKIQQSPDYSEEMDFEAGVRDFRDRLANYEKVYEPVEE
GSYIKMIDMVSGNGGQIQVNNISGYLPGRIVFFLVNTHLTPRPILLTRHG
ESMDNVRGRIGGDSVISDSGKLYAKKLASFVEKRLKSEKAASIWTSTLQR
TNLTASSIVGFPKVQWRALDEINAGVCDGMTYEEVKKNMPEEYESRKKDK
LRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFAD
RPLKEIPQIEMPLHTIIEIQMGVSGVQEKRYKLMD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5234
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express At F2KP with N-terminal GST (contains precission protease site); N.B. Sequence differences compared with AF190739 CDS: ; a516g (silent P172) ; c1886a (A629D) - present in several other published mRNA sequences; ; ; Estimated MW of expressed protein : 110269 Daltons