cDNA Clone HIPK4

• Plasmid: pGEX6P-1-HIPK4
• Parent Plasmid: pGEX6P1
• Expressed: GST-HIPK4
• Genbank: NM_144685
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGTCCACCATCCAGTCGGAGACTGACTGCTACGACATCATCGAGGTCTTGGGCAAGGGGACCTTCGGGGAGGTAGCCAAGGGCTGGCGGCGGAGCACGGGCGAGATGGTGGCCATCAAGATCCTCAAGAATGACGCCTACCGCAACCGCATCATCAAAAACGAGCTGAAGCTGCTGCACTGCATGCGAGGCCTAGACCCTGAAGAGGCCCACGTCATCCGCTTCCTTGAGTTCTTCCATGACGCCCTCAAGTTCTACCTGGTCTTTGAGCTGCTGGAGCAAAACCTTTTCGAGTTCCAG
AAGGAGAACAACTTCGCGCCCCTCCCCGCCCGCCACATCCGTACAGTCAC
CCTGCAGGTGCTCACAGCCCTGGCCCGGCTCAAGGAGCTGGCTATCATCC
ACGCTGATCTCAAGCCTGAGAACATCATGCTGGTGGACCAGACCCGCTGC
CCCTTCAGGGTCAAGGTGATTGACTTCGGATCCGCCAGCATTTTCAGCGA
GGTGCGCTACGTGAAGGAGCCATACATCCAGTCGCGCTTCTACCGGGCCC
CTGAGATCCTGCTGGGGCTGCCCTTCTGCGAGAAGGTGGACGTGTGGTCC
CTGGGCTGCGTCATGGCTGAGCTGCACCTGGGCTGGCCTCTCTACCCCGG
CAACAACGAGTACGACCAGGTGCGCTACATCTGCGAAACCCAGGGCCTGC
CCAAGCCACACCTGTTGCACGCCGCCTGCAAGGCCCACCACTTCTTCAAG
CGCAACCCCCACCCTGACGCTGCCAACCCCTGGCAGCTCAAGTCCTCGGC
TGACTACCTGGCCGAGACGAAGGTGCGCCCATTGGAGCGCCGCAAGTATA
TGCTCAAGTCGTTGGACCAGATTGAGACAGTGAATGGTGGCAGTGTGGCC
AGTCGGCTAACCTTCCCTGACCGGGAGGCGCTGGCGGAGCACGCCGACCT
CAAGAGCATGGTGGAGCTGATCAAGCGCATGCTGACCTGGGAGTCACACG
AACGCATCAGCCCCAGTGCTGCCCTGCGCCACCCCTTCGTGTCCATGCAG
CAGCTGCGCAGTGCCCACGAGACCACCCACTACTACCAGCTCTCGCTGCG
CAGCTACCGCCTCTCGCTGCAAGTGGAGGGGAAGCCCCCCACGCCCGTCG
TGGCCGCAGAAGATGGGACCCCCTACTACTGTCTGGCTGAGGAGAAGGAG
GCTGCGGGTATGGGCAGTGTGGCCGGCAGCAGCCCCTTCTTCCGAGAGGA
GAAGGCACCAGGTATGCAAAGAGCCATCGACCAGCTGGATGACCTGAGTC
TGCAGGAGGCTGGGCATGGGCTGTGGGGTGAGACCTGCACCAATGCGGTC
TCCGACATGATGGTCCCCCTCAAGGCAGCCATCACTGGCCACCATGTGCC
CGACTCGGGCCCTGAGCCCATCCTGGCCTTCTACAGCAGCCGCCTGGCAG
GCCGCCACAAGGCCCGCAAGCCACCTGCGGGTTCCAAGTCCGACTCCAAC
TTCAGCAACCTCATTCGGCTGAGCCAGGTCTCGCCTGAGGATGACAGGCC
CTGCCGGGGCAGCAGCTGGGAGGAAGGAGAGCATCTCGGGGCCTCTGCTG
AGCCACTGGCCATCCTGCAGCGAGATGAGGATGGGCCCAACATTGACAAC
ATGACCATGGAAGCTGAGAGGCCAGACCCTGAGCTCTTCGACCCCAGCAG
CTGTCCTGGAGAATGGCTGAGTGAGCCAGACTGCACCCTGGAGAGCGTCA
GGGGCCCACGGGCTCAGGGGCTCCCACCCCGCCGCTCCCACCAGCATGGT
CCACCCCGGGGGGCCACCAGCTTCCTCCAGCATGTCACCGGGCACCACTG
Agcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMSTIQSETDCYDIIEV
LGKGTFGEVAKGWRRSTGEMVAIKILKNDAYRNRIIKNELKLLHCMRGLD
PEEAHVIRFLEFFHDALKFYLVFELLEQNLFEFQKENNFAPLPARHIRTV
TLQVLTALARLKELAIIHADLKPENIMLVDQTRCPFRVKVIDFGSASIFS
EVRYVKEPYIQSRFYRAPEILLGLPFCEKVDVWSLGCVMAELHLGWPLYP
GNNEYDQVRYICETQGLPKPHLLHAACKAHHFFKRNPHPDAANPWQLKSS
ADYLAETKVRPLERRKYMLKSLDQIETVNGGSVASRLTFPDREALAEHAD
LKSMVELIKRMLTWESHERISPSAALRHPFVSMQQLRSAHETTHYYQLSL
RSYRLSLQVEGKPPTPVVAAEDGTPYYCLAEEKEAAGMGSVAGSSPFFRE
EKAPGMQRAIDQLDDLSLQEAGHGLWGETCTNAVSDMMVPLKAAITGHHV
PDSGPEPILAFYSSRLAGRHKARKPPAGSKSDSNFSNLIRLSQVSPEDDR
PCRGSSWEEGEHLGASAEPLAILQRDEDGPNIDNMTMEAERPDPELFDPS
SCPGEWLSEPDCTLESVRGPRAQGLPPRRSHQHGPPRGATSFLQHVTGHH
*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5228
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 96560 Daltons