cDNA Clone HIPK4

• Plasmid: pFBHTa HIPK4
• Parent Plasmid: pSC-A
• Expressed: 6His-HIPK4
• Genbank: NM_144685
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcATGTCCACCATCCAGTCGGAGACTGACTGCTACGACATCATCGAGGTCTTGGGCAAGGGGACCTTCGGGGAGGTAGCCAAGGGCTGGCGGCGGAGCACGGGCGAGATGGTGGCCATCAAGATCCTCAAGAATGACGCCTACCGCAACCGCATCATCAAAAACGAGCTGAAGCTGCTGCACTGCATGCGAGGCCTAGACCCTGAAGAGGCCCACGTCATCCGCTTCCTTGAGTTCTTCCATGACGCCCTCAAGTTCTACCTGGTCTTTGAGCTGCTGGAGCAAAACCTTTTCGAGTTCCAG
AAGGAGAACAACTTCGCGCCCCTCCCCGCCCGCCACATCCGTACAGTCAC
CCTGCAGGTGCTCACAGCCCTGGCCCGGCTCAAGGAGCTGGCTATCATCC
ACGCTGATCTCAAGCCTGAGAACATCATGCTGGTGGACCAGACCCGCTGC
CCCTTCAGGGTCAAGGTGATTGACTTCGGATCCGCCAGCATTTTCAGCGA
GGTGCGCTACGTGAAGGAGCCATACATCCAGTCGCGCTTCTACCGGGCCC
CTGAGATCCTGCTGGGGCTGCCCTTCTGCGAGAAGGTGGACGTGTGGTCC
CTGGGCTGCGTCATGGCTGAGCTGCACCTGGGCTGGCCTCTCTACCCCGG
CAACAACGAGTACGACCAGGTGCGCTACATCTGCGAAACCCAGGGCCTGC
CCAAGCCACACCTGTTGCACGCCGCCTGCAAGGCCCACCACTTCTTCAAG
CGCAACCCCCACCCTGACGCTGCCAACCCCTGGCAGCTCAAGTCCTCGGC
TGACTACCTGGCCGAGACGAAGGTGCGCCCATTGGAGCGCCGCAAGTATA
TGCTCAAGTCGTTGGACCAGATTGAGACAGTGAATGGTGGCAGTGTGGCC
AGTCGGCTAACCTTCCCTGACCGGGAGGCGCTGGCGGAGCACGCCGACCT
CAAGAGCATGGTGGAGCTGATCAAGCGCATGCTGACCTGGGAGTCACACG
AACGCATCAGCCCCAGTGCTGCCCTGCGCCACCCCTTCGTGTCCATGCAG
CAGCTGCGCAGTGCCCACGAGACCACCCACTACTACCAGCTCTCGCTGCG
CAGCTACCGCCTCTCGCTGCAAGTGGAGGGGAAGCCCCCCACGCCCGTCG
TGGCCGCAGAAGATGGGACCCCCTACTACTGTCTGGCTGAGGAGAAGGAG
GCTGCGGGTATGGGCAGTGTGGCCGGCAGCAGCCCCTTCTTCCGAGAGGA
GAAGGCACCAGGTATGCAAAGAGCCATCGACCAGCTGGATGACCTGAGTC
TGCAGGAGGCTGGGCATGGGCTGTGGGGTGAGACCTGCACCAATGCGGTC
TCCGACATGATGGTCCCCCTCAAGGCAGCCATCACTGGCCACCATGTGCC
CGACTCGGGCCCTGAGCCCATCCTGGCCTTCTACAGCAGCCGCCTGGCAG
GCCGCCACAAGGCCCGCAAGCCACCTGCGGGTTCCAAGTCCGACTCCAAC
TTCAGCAACCTCATTCGGCTGAGCCAGGTCTCGCCTGAGGATGACAGGCC
CTGCCGGGGCAGCAGCTGGGAGGAAGGAGAGCATCTCGGGGCCTCTGCTG
AGCCACTGGCCATCCTGCAGCGAGATGAGGATGGGCCCAACATTGACAAC
ATGACCATGGAAGCTGAGAGGCCAGACCCTGAGCTCTTCGACCCCAGCAG
CTGTCCTGGAGAATGGCTGAGTGAGCCAGACTGCACCCTGGAGAGCGTCA
GGGGCCCACGGGCTCAGGGGCTCCCACCCCGCCGCTCCCACCAGCATGGT
CCACCCCGGGGGGCCACCAGCTTCCTCCAGCATGTCACCGGGCACCACTG
Agcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMSTIQSETDCYDIIEVLGKG
TFGEVAKGWRRSTGEMVAIKILKNDAYRNRIIKNELKLLHCMRGLDPEEA
HVIRFLEFFHDALKFYLVFELLEQNLFEFQKENNFAPLPARHIRTVTLQV
LTALARLKELAIIHADLKPENIMLVDQTRCPFRVKVIDFGSASIFSEVRY
VKEPYIQSRFYRAPEILLGLPFCEKVDVWSLGCVMAELHLGWPLYPGNNE
YDQVRYICETQGLPKPHLLHAACKAHHFFKRNPHPDAANPWQLKSSADYL
AETKVRPLERRKYMLKSLDQIETVNGGSVASRLTFPDREALAEHADLKSM
VELIKRMLTWESHERISPSAALRHPFVSMQQLRSAHETTHYYQLSLRSYR
LSLQVEGKPPTPVVAAEDGTPYYCLAEEKEAAGMGSVAGSSPFFREEKAP
GMQRAIDQLDDLSLQEAGHGLWGETCTNAVSDMMVPLKAAITGHHVPDSG
PEPILAFYSSRLAGRHKARKPPAGSKSDSNFSNLIRLSQVSPEDDRPCRG
SSWEEGEHLGASAEPLAILQRDEDGPNIDNMTMEAERPDPELFDPSSCPG
EWLSEPDCTLESVRGPRAQGLPPRRSHQHGPPRGATSFLQHVTGHH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5222
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 73094 Daltons