cDNA Clone HIPK4

• Plasmid: pEBG6P-1 HIPK4
• Parent Plasmid: pEBG6P
• Expressed: GST-HIPK4
• Genbank: NM_144685
• Tag: GST
• Species: Human

Sequence of Insert

actagtATGTCCACCATCCAGTCGGAGACTGACTGCTACGACATCATCGAGGTCTTGGGCAAGGGGACCTTCGGGGAGGTAGCCAAGGGCTGGCGGCGGAGCACGGGCGAGATGGTGGCCATCAAGATCCTCAAGAATGACGCCTACCGCAACCGCATCATCAAAAACGAGCTGAAGCTGCTGCACTGCATGCGAGGCCTAGACCCTGAAGAGGCCCACGTCATCCGCTTCCTTGAGTTCTTCCATGACGCCCTCAAGTTCTACCTGGTCTTTGAGCTGCTGGAGCAAAACCTTTTCGAGTTCCAG
AAGGAGAACAACTTCGCGCCCCTCCCCGCCCGCCACATCCGTACAGTCAC
CCTGCAGGTGCTCACAGCCCTGGCCCGGCTCAAGGAGCTGGCTATCATCC
ACGCTGATCTCAAGCCTGAGAACATCATGCTGGTGGACCAGACCCGCTGC
CCCTTCAGGGTCAAGGTGATTGACTTCGGATCCGCCAGCATTTTCAGCGA
GGTGCGCTACGTGAAGGAGCCATACATCCAGTCGCGCTTCTACCGGGCCC
CTGAGATCCTGCTGGGGCTGCCCTTCTGCGAGAAGGTGGACGTGTGGTCC
CTGGGCTGCGTCATGGCTGAGCTGCACCTGGGCTGGCCTCTCTACCCCGG
CAACAACGAGTACGACCAGGTGCGCTACATCTGCGAAACCCAGGGCCTGC
CCAAGCCACACCTGTTGCACGCCGCCTGCAAGGCCCACCACTTCTTCAAG
CGCAACCCCCACCCTGACGCTGCCAACCCCTGGCAGCTCAAGTCCTCGGC
TGACTACCTGGCCGAGACGAAGGTGCGCCCATTGGAGCGCCGCAAGTATA
TGCTCAAGTCGTTGGACCAGATTGAGACAGTGAATGGTGGCAGTGTGGCC
AGTCGGCTAACCTTCCCTGACCGGGAGGCGCTGGCGGAGCACGCCGACCT
CAAGAGCATGGTGGAGCTGATCAAGCGCATGCTGACCTGGGAGTCACACG
AACGCATCAGCCCCAGTGCTGCCCTGCGCCACCCCTTCGTGTCCATGCAG
CAGCTGCGCAGTGCCCACGAGACCACCCACTACTACCAGCTCTCGCTGCG
CAGCTACCGCCTCTCGCTGCAAGTGGAGGGGAAGCCCCCCACGCCCGTCG
TGGCCGCAGAAGATGGGACCCCCTACTACTGTCTGGCTGAGGAGAAGGAG
GCTGCGGGTATGGGCAGTGTGGCCGGCAGCAGCCCCTTCTTCCGAGAGGA
GAAGGCACCAGGTATGCAAAGAGCCATCGACCAGCTGGATGACCTGAGTC
TGCAGGAGGCTGGGCATGGGCTGTGGGGTGAGACCTGCACCAATGCGGTC
TCCGACATGATGGTCCCCCTCAAGGCAGCCATCACTGGCCACCATGTGCC
CGACTCGGGCCCTGAGCCCATCCTGGCCTTCTACAGCAGCCGCCTGGCAG
GCCGCCACAAGGCCCGCAAGCCACCTGCGGGTTCCAAGTCCGACTCCAAC
TTCAGCAACCTCATTCGGCTGAGCCAGGTCTCGCCTGAGGATGACAGGCC
CTGCCGGGGCAGCAGCTGGGAGGAAGGAGAGCATCTCGGGGCCTCTGCTG
AGCCACTGGCCATCCTGCAGCGAGATGAGGATGGGCCCAACATTGACAAC
ATGACCATGGAAGCTGAGAGGCCAGACCCTGAGCTCTTCGACCCCAGCAG
CTGTCCTGGAGAATGGCTGAGTGAGCCAGACTGCACCCTGGAGAGCGTCA
GGGGCCCACGGGCTCAGGGGCTCCCACCCCGCCGCTCCCACCAGCATGGT
CCACCCCGGGGGGCCACCAGCTTCCTCCAGCATGTCACCGGGCACCACTG
Agcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMSTIQSETDCY
DIIEVLGKGTFGEVAKGWRRSTGEMVAIKILKNDAYRNRIIKNELKLLHC
MRGLDPEEAHVIRFLEFFHDALKFYLVFELLEQNLFEFQKENNFAPLPAR
HIRTVTLQVLTALARLKELAIIHADLKPENIMLVDQTRCPFRVKVIDFGS
ASIFSEVRYVKEPYIQSRFYRAPEILLGLPFCEKVDVWSLGCVMAELHLG
WPLYPGNNEYDQVRYICETQGLPKPHLLHAACKAHHFFKRNPHPDAANPW
QLKSSADYLAETKVRPLERRKYMLKSLDQIETVNGGSVASRLTFPDREAL
AEHADLKSMVELIKRMLTWESHERISPSAALRHPFVSMQQLRSAHETTHY
YQLSLRSYRLSLQVEGKPPTPVVAAEDGTPYYCLAEEKEAAGMGSVAGSS
PFFREEKAPGMQRAIDQLDDLSLQEAGHGLWGETCTNAVSDMMVPLKAAI
TGHHVPDSGPEPILAFYSSRLAGRHKARKPPAGSKSDSNFSNLIRLSQVS
PEDDRPCRGSSWEEGEHLGASAEPLAILQRDEDGPNIDNMTMEAERPDPE
LFDPSSCPGEWLSEPDCTLESVRGPRAQGLPPRRSHQHGPPRGATSFLQH
VTGHH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5221
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express HIPK4 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 96985 Daltons