cDNA Clone Cyclin D1

• Plasmid: pFastbac GST Cyclin D1
• Parent Plasmid: pFastbac GST
• Expressed: GST-Cyclin D1
• Genbank: NM_053056
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGAACACCAGCTCCTGTGCTGCGAAGTGGAAACCATCCGCCGCGCGTACCCCGATGCCAACCTCCTCAACGACCGGGTGCTGCGGGCCATGCTGAAGGCGGAGGAGACCTGCGCGCCCTCGGTGTCCTACTTCAAATGTGTGCAGAAG
GAGGTCCTGCCGTCCATGCGGAAGATCGTCGCCACCTGGATGCTGGAGGT
CTGCGAGGAACAGAAGTGCGAGGAGGAGGTCTTCCCGCTGGCCATGAACT
ACCTGGACCGCTTCCTGTCGCTGGAGCCCGTGAAAAAGAGCCGCCTGCAG
CTGCTGGGGGCCACTTGCATGTTCGTGGCCTCTAAGATGAAGGAGACCAT
CCCCCTGACGGCCGAGAAGCTGTGCATCTACACCGACAACTCCATCCGGC
CCGAGGAGCTGCTGCAAATGGAGCTGCTCCTGGTGAACAAGCTCAAGTGG
AACCTGGCCGCAATGACCCCGCACGATTTCATTGAACACTTCCTCTCCAA
AATGCCAGAGGCGGAGGAGAACAAACAGATCATCCGCAAACACGCGCAGA
CCTTCGTTGCCCTCTGTGCCACAGATGTGAAGTTCATTTCCAATCCGCCC
TCCATGGTGGCAGCGGGGAGCGTGGTGGCCGCAGTGCAAGGCCTGAACCT
GAGGAGCCCCAACAACTTCCTGTTCTACTACCGCCTCACACGCTTCCTCT
CCAGAGTGATCAAGTGTGACCCAGACTGCCTCCGGGCCTGCCAGGAGCAG
ATCGAAGCCCTGCTGGAGTCAAGCCTGCGCCAGGCCCAGCAGAACATGGA
CCCCAAGGCCGCCGAGGAGGAGGAAGAGGAGGAGGAGGAGGTGGACCTGG
CTTGCACACCCACCGACGTGCGGGACGTGGACATCTGAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEHQLLCCEVETIRRAYPDA
NLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEE
QKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLT
AEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPE
AEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSP
NNFLFYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKA
AEEEEEEEEEVDLACTPTDVRDVDI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5121
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express GST N terminal fusion.; N.B. Sequence difference compared with NM_053056 CDS:; c674t (S225F).; ; Estimated MW of expressed protein : 60442 Daltons