cDNA Clone RIPK2

• Plasmid: pGEX6P-1-RIPK2/RICK
• Parent Plasmid: pGEX6P1
• Expressed: GST-RIPK2
• Genbank: NM_003821
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTCCCTACCACAAACTCGCCGACCTGCGCTACCTGAGCCGCGGCGCCTCTGGCACTGTGTCGTCCGCCCGCCACGCAGACTGGCGCGTCCAGGTGGCCGTGAAGCACCTGCACATCCACACTCCGCTGCTCGACAGTGAAAGAAAGGATGTCTTAAGAGAAGCTGAAATTTTACACAAAGCTAGATTTAGTTACATTCTTCCAATTTTGGGAATTTGCAATGAGCCTGAATTTTTGGGAATAGTTACTGAATACATGCCAAAT
GGATCATTAAATGAACTCCTACATAGGAAAACTGAATATCCTGATGTTGC
TTGGCCATTGAGATTTCGCATCCTGCATGAAATTGCCCTTGGTGTAAATT
ACCTGCACAATATGACTCCTCCTTTACTTCATCATGACTTGAAGACTCAG
AATATCTTATTGGACAATGAATTTCATGTTAAGATTGCAGATTTTGGTTT
ATCAAAGTGGCGCATGATGTCCCTCTCACAGTCACGAAGTAGCAAATCTG
CACCAGAAGGAGGGACAATTATCTATATGCCACCTGAAAACTATGAACCT
GGACAAAAATCAAGGGCCAGTATCAAGCACGATATATATAGCTATGCAGT
TATCACATGGGAAGTGTTATCCAGAAAACAGCCTTTTGAAGATGTCACCA
ATCCTTTGCAGATAATGTATAGTGTGTCACAAGGACATCGACCTGTTATT
AATGAAGAAAGTTTGCCATATGATATACCTCACCGAGCACGTATGATCTC
TCTAATAGAAAGTGGATGGGCACAAAATCCAGATGAAAGACCATCTTTCT
TAAAATGTTTAATAGAACTTGAACCAGTTTTGAGAACATTTGAAGAGATA
ACTTTTCTTGAAGCTGTTATTCAGCTAAAGAAAACAAAGTTACAGAGTGT
TTCAAGTGCCATTCACCTATGTGACAAGAAGAAAATGGAATTATCTCTGA
ACATACCTGTAAATCATGGTCCACAAGAGGAATCATGTGGATCCTCTCAG
CTCCATGAAAATAGTGGTTCTCCTGAAACTTCAAGGTCCCTGCCAGCTCC
TCAAGACAATGATTTTTTATCTAGAAAAGCTCAAGACTGTTATTTTATGA
AGCTGCATCACTGTCCTGGAAATCACAGTTGGGATAGCACCATTTCTGGA
TCTCAAAGGGCTGCATTCTGTGATCACAAGACCACTCCATGCTCTTCAGC
AATAATAAATCCACTCTCAACTGCAGGAAACTCAGAACGTCTGCAGCCTG
GTATAGCCCAGCAGTGGATCCAGAGCAAAAGGGAAGACATTGTGAACCAA
ATGACAGAAGCCTGCCTTAACCAGTCGCTAGATGCCCTTCTGTCCAGGGA
CTTGATCATGAAAGAGGACTATGAACTTGTTAGTACCAAGCCTACAAGGA
CCTCAAAAGTCAGACAATTACTAGACACTACTGACATCCAAGGAGAAGAA
TTTGCCAAAGTTATAGTACAAAAATTGAAAGATAACAAACAAATGGGTCT
TCAGCCTTACCCGGAAATACTTGTGGTTTCTAGATCACCATCTTTAAATT
TACTTCAAAATAAAAGCATGTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMNGEAICSALPTIPYH
KLADLRYLSRGASGTVSSARHADWRVQVAVKHLHIHTPLLDSERKDVLRE
AEILHKARFSYILPILGICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDV
AWPLRFRILHEIALGVNYLHNMTPPLLHHDLKTQNILLDNEFHVKIADFG
LSKWRMMSLSQSRSSKSAPEGGTIIYMPPENYEPGQKSRASIKHDIYSYA
VITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMI
SLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQS
VSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQLHENSGSPETSRSLPA
PQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSS
AIINPLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSR
DLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMG
LQPYPEILVVSRSPSLNLLQNKSM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5093
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 88335 Daltons