cDNA Clone BRSK2

• Plasmid: pGEX6P-1-BRSK2 1-392 (kinase domain)
• Parent Plasmid: pGEX6P1
• Expressed: GST-BRSK2 1-392 (kinase domain)
• Genbank: AF533878
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGACATCGACGGGGAAGGACGGCGGCGCGCAGCACGCGCAGTA
TGTTGGGCCCTACCGGCTGGAGAAGACGCTGGGCAAGGGGCAGACAGGTC
TGGTGAAGCTGGGGGTTCACTGCGTCACCTGCCAGAAGGTGGCCATCAAG
ATCGTCAACCGTGAGAAGCTCAGCGAGTCGGTGCTGATGAAGGTGGAGCG
GGAGATCGCGATCCTGAAGCTCATTGAGCACCCCCACGTCCTAAAGCTGC
ACGACGTTTATGAAAACAAAAAATATTTGTACCTGGTGCTAGAACACGTG
TCAGGTGGTGAGCTCTTCGACTACCTGGTGAAGAAGGGGAGGCTGACGCC
TAAGGAGGCTCGGAAGTTCTTCCGGCAGATCATCTCTGCGCTGGACTTCT
GCCACAGCCACTCCATATGCCACAGGGATCTGAAACCTGAAAACCTCCTG
CTGGACGAGAAGAACAACATCCGCATCGCAGACTTTGGCATGGCGTCCCT
GCAGGTTGGCGACAGCCTGTTGGAGACCAGCTGTGGGTCCCCCCACTACG
CCTGCCCCGAGGTGATCCGGGGGGAGAAGTATGACGGCCGGAAGGCGGAC
GTGTGGAGCTGCGGCGTCATCCTGTTCGCCTTGCTGGTGGGGGCTCTGCC
CTTCGACGATGACAACTTGCGACAGCTGCTGGAGAAGGTGAAGCGGGGCG
TGTTCCACATGCCGCACTTTATCCCGCCCGACTGCCAGAGTCTGCTACGG
GGCATGATCGAGGTGGACGCCGCACGCCGCCTCACGCTAGAGCACATTCA
GAAACACATATGGTATATAGGGGGCAAGAATGAGCCCGAACCAGAGCAGC
CGATTCCTCGCAAGGTGCAGATCCGCTCGCTGCCCAGCCTGGAGGACATC
GACCCCGACGTGCTGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCG
CAACAAGCTGCTGCAGGACCTGCTGTCCGAGGAGGAGAACCAGGAGAAGA
TGATTTACTTCCTCCTCCTGGACCGGAAAGAAAGGTACCCGAGCCAGGAG
GATGAGGACCTGCCCCCCCGGAACGAGATAGACCCTCCCCGGAAGCGTGT
GGACTCCCCGATGCTGAACCGGCACGGCAAGCGGCGGCCAGAACGCAAAT
CCATGGAGGTGCTCAGCGTGACGGACGGCGGCTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTSTGKDGGAQHAQYVGPY
RLEKTLGKGQTGLVKLGVHCVTCQKVAIKIVNREKLSESVLMKVEREIAI
LKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEAR
KFFRQIISALDFCHSHSICHRDLKPENLLLDEKNNIRIADFGMASLQVGD
SLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDD
NLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIW
YIGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLL
QDLLSEEENQEKMIYFLLLDRKERYPSQEDEDLPPRNEIDPPRKRVDSPM
LNRHGKRRPERKSMEVLSVTDGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5091
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion. ; N.B. Changes to AF533878 CDS sequence:; g752t (S251I)*, c846g (silent), a1485g (silent)*; *present in BRSK2 sequence in Sugen database ; Estimated MW of expressed protein : 71669 Daltons