cDNA Clone FKHRL1

• Plasmid: pCMV5-FLAG FKHRL1 S7A S12A
• Parent Plasmid: pCMV5
• Expressed: FLAG-FKHRL1 S7A S12A
• Genbank: NM_001455
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccgccaccatgGACTACAAGGACGACGATGACAAGgcagaggcacc
ggctgccccggccccgctcgctccgctcgaagtggagctggacccggagt
tcgagccccagagccgtccgcgatcctgtacgtggcccctgcaaaggccg
gagctccaagcgagccctgccaagccctcgggggagacggccgccgactc
catgatccccgaggaggaggacgatgaagacgacgaggacggcgggggac
gggccggctcggccatggcgatcggcggcggcggcgggagcggcacgctg
ggctccgggctgctccttgaggactcggcccgggtgctggcacccggagg
gcaagaccccgggtctgggccagccaccgcggcgggcgggctgagcgggg
gtacacaggcgctgctgcagcctcagcaaccgctgccaccgccgcagccg
ggggcggctgggggctccgggcagccgaggaaatgttcgtcgcggcggaa
cgcctggggaaacctgtcctacgcggacctgatcacccgcgccatcgaga
gctccccggacaaacggctcactctgtcccagatctacgagtggatggtg
cgttgcgtgccctacttcaaggataagggcgacagcaacagctctgccgg
ctggaagaactccatccggcacaacctgtcactgcatagtcgattcatgc
gggtccagaatgagggaactggcaagagctcttggtggatcatcaaccct
gatggggggaagagcggaaaagccccccggcggcgggctgtctccatgga
caatagcaacaagtataccaagagccgtggccgcgcagccaagaagaagg
cagccctgcagacagcccccgaatcagctgacgacagtccctcccagctc
tccaagtggcctggcagccccacgtcacgcagcagtgatgagctggatgc
gtggacggacttccgttcacgcaccaattctaacgccagcacagtcagtg
gccgcctgtcgcccatcatggcaagcacagagttggatgaagtccaggac
gatgatgcgcctctctcgcccatgctctacagcagctcagccagcctgtc
accttcagtaagcaagccgtgcacggtggaactgccacggctgactgata
tggcaggcaccatgaatctgaatgatgggctgactgaaaacctcatggac
gacctgctggataacatcacgctcccgccatcccagccatcgcccactgg
gggactcatgcagcggagctctagcttcccgtataccaccaagggctcgg
gcctgggctccccaaccagctcctttaacagcacggtgttcggaccttca
tctctgaactccctacgccagtctcccatgcagaccatccaagagaacaa
gccagctaccttctcttccatgtcacactatggtaaccagacactccagg
acctgctcacttcggactcacttagccacagcgatgtcatgatgacacag
tcggaccccttgatgtctcaggccagcaccgctgtgtctgcccagaattc
ccgccggaacgtgatgcttcgcaatgatccgatgatgtcctttgctgccc
agcctaaccagggaagtttggtcaatcagaacttgctccaccaccagcac
caaacccagggcgctcttggtggcagccgtgccttgtcgaattctgtcag
caacatgggcttgagtgagtccagcagccttgggtcagccaaacaccagc
agcagtctcctgtcagccagtctatgcaaaccctctcggactctctctca
ggctcctccttgtactcaactagtgcaaacctgcccgtcatgggccatga
gaagttccccagcgacttggacctggacatgttcaatgggagcttggaat
gtgacatggagtccattatccgtagtgaactcatggatgctgatgggttg
gattttaactttgattccctcatctccacacagaatgttgttggtttgaa
cgtggggaacttcactggtgctaagcaggcctcatctcagagctgggtgc
caggctgaggatcc

Amino Acid Sequence

MDYKDDDDKAEAPAAPAPLAPLEVELDPEFEPQSRPRSCTWPLQRPELQA
SPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGL
LLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAG
GSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVP
YFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGK
SGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWP
GSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAP
LSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLD
NITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNS
LRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPL
MSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQG
ALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSL
YSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNF
DSLISTQNVVGLNVGNFTGAKQASSQSWVPG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5054
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Change to NM_001455 orf sequence: t159c (silent in A53) – natural variant.; Estimated MW of expressed protein : 72654 Daltons