cDNA Clone BS69

• Plasmid: pFastBacHTb BS69
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-BS69
• Genbank: NM_006624.2 GI:47078241
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccTCTCGAGTCCACGGTATGCACCCTAAAGAGACCACCCGTCAGCTGAGCTTAGCTGTGAAAGATGGTCTTATTGTCGAAACTCTAACAGTGGGCTGCAAAGGTTCAAAAGCTGGTATTGAACAAGAAGGATATTGGTTGCCAGGAGAT
GAGATTGACTGGGAAACAGAAAATCATGACTGGTATTGTTTTGAATGCCATTTGCCTGGAGAGGTGTTGATATGTGACCTGTGTTTTCGTGTGTATCATTCCAAGTGTTTGTCTGATGAGTTCAGGCTTAGAGACAGCAGTAGTCCCTGGCAGTGCCCAGTTTGCAGGAGCATTAAGAAGAAGAATACAAACAAACAGGAGATGGGCACATACCTCAGATTCATTGTCTCCCGCATGAAGGAGAGGGCTATAGATCTTAATAAAAAGGGGAAGGACAATAAACACCCGATGTACAGGAGGCTGGTGCACTCAGCTGTGGACGTTCCCACCATTCAAGAGAAAGTGAATGAAGGGAAATACCGAAGTTATGAAGAGTTCAAAGCTGATGCCCAATTGCTTCTCCACAATACCGTGATTTTCTATGGAGCAGACAGTGAGCAAGCTGACATTGCGAGGATGCTATATAAAGACACATGTCATGAGCTGGATGAACTGCAGCTTTGCAAGAATTGCTTTTACTTGTCAAATGCTCGTCCTGACAACTGGTTCTGTTATCCTTGTATACCTAATCATGAGCTGGTTTGGGCTAAAATGAAAGGTTTTGGGTTTTGGCCAGCCAAAGTCATGCAGAAAGAAGACAATCAAGTCGACGTTCGCTTCTTTGGCCACCACCACCAGAGGGCCTGGATTCCTTCTGAAAACATTCAAGATATCACAGTCAACATTCATCGGCTGCACGTGAAGCGCAGTATGGGTTGGAAAAAGGCCTGTGATGAGCTGGAGCTGCATCAGCGTTTCCTACGAGAAGGGAGATTTTGGAAATCTAAGAATGAGGACCGAGGTGAGGAAGAGGCAGAATCCAGTATCTCCTCCACCAGTAATGAGCAGCTAAAGGTCACTCAAGAACCAAGAGCAAAGAAAGGACGACGTAATCAAAGTGTGGAGCCCAAAAAGGAAGAACCAGAGCCTGAAACAGAAGCAGTAAGTTCTAGCCAGGAAATACCCACGATGCCTCAGCCCATCGAAAAAGTCTCCGTGTCAACTCAGACAAAGAAGTTAAGTGCCTCTTCACCAAGAATGCTGCATCGGAGCACCCAGACCACAAACGACGGCGTGTGTCAGAGCATGTGCCATGACAAATACACCAAGATCTTCAATGACTTCAAAGACCGGATGAAGTCGGACCACAAGCGGGAGACAGAGCGTGTTGTCCGAGAAGCTCTGGAGAAGCTGCGTTCTGAAATGGAAGAAGAAAAGAGACAAGCTGTAAATAAAGCTGTAGCCAACATGCAGGGTGAGATGGACAGAAAATGTAAGCAAGTAAAGGAAAAGTGTAAGGAGGAATTTGTAGAAGAAATCAAGAAGCTGGCAACACAGCACAAGCAACTGATTTCTCAGACCAAGAAGAAGCAGTGGTGCTACAACTGTGAGGAGGAGGCCATGTACCACTGCTGCTGGAACACATCCTACTGCTCCATCAAGTGCCAGCAGGAGCACTGGCACGCGGAGCACAAGCGCACCTGCCGCCGGAAAAGATGAgcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSSRVHGMHPKETTRQLSLAVKDG
LIVETLTVGCKGSKAGIEQEGYWLPGDEIDWETENHDWYCFECHLPGEVL
ICDLCFRVYHSKCLSDEFRLRDSSSPWQCPVCRSIKKKNTNKQEMGTYLR
FIVSRMKERAIDLNKKGKDNKHPMYRRLVHSAVDVPTIQEKVNEGKYRSY
EEFKADAQLLLHNTVIFYGADSEQADIARMLYKDTCHELDELQLCKNCFY
LSNARPDNWFCYPCIPNHELVWAKMKGFGFWPAKVMQKEDNQVDVRFFGH
HHQRAWIPSENIQDITVNIHRLHVKRSMGWKKACDELELHQRFLREGRFW
KSKNEDRGEEEAESSISSTSNEQLKVTQEPRAKKGRRNQSVEPKKEEPEP
ETEAVSSSQEIPTMPQPIEKVSVSTQTKKLSASSPRMLHRSTQTTNDGVC
QSMCHDKYTKIFNDFKDRMKSDHKRETERVVREALEKLRSEMEEEKRQAV
NKAVANMQGEMDRKCKQVKEKCKEEFVEEIKKLATQHKQLISQTKKKQWC
YNCEEEAMYHCCWNTSYCSIKCQQEHWHAEHKRTCRRKR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1474
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid to express BS69 with N terminal 6His tag.; Estimated MW of expressed protein : 69398 Daltons