cDNA Clone BRSK1

• Plasmid: pGEX6P-1-HA-BRSK1 304-366
• Parent Plasmid: pGEX6P1
• Expressed: GST-HA-BRSK1 304-366
• Genbank: NM_032430
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

ggatccGCCACCATGTACCCATACGATGTGCCAGATTACGCCGTAGCCAT
GCGGAGCCTGCCATCCAACGGAGAGCTGGACCCCGACGTCCTAGAGAGCA
TGGCATCACTGGGCTGCTTCAGGGACCGCGAGAGGCTGCATCGCGAGCTG
CGCAGTGAGGAGGAGAACCAAGAAAAGATGATATATTATCTGCTTTTGGA
TCGGAAGGAGCGGTATCCCAGCTGTGAGGACTGAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMYPYDVPDYAVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCED*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1351
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express BRSK1 UBA domain with N-terminal GST and HA tags