cDNA Clone MARK4

• Plasmid: pFastBac.HTb MARK4
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-MARK4
• Genbank: AK075272
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccTCTTCGCGGACGGTGCTGGCCCCGGGCAACGATCGGAACTCGGACACGCATGGCACCTTGGGCAGTGGCCGCTCCTCGGACAAAGGCCCGTCCTGGTCCAGCCGCTCACTGGGTGCCCGTTGCCGGAACTCCATCGCCTCCTGTCCC
GAGGAGCAGCCCCACGTGGGCAACTACCGCCTGCTGAGGACCATTGGGAA
GGGCAACTTTGCCAAAGTCAAGCTGGCTCGGCACATCCTCACTGGTCGGG
AGGTTGCCATCAAGATTATCGACAAAACCCAGCTGAATCCCAGCAGCCTG
CAGAAGCTGTTCCGAGAAGTCCGCATCATGAAGGGCCTAAACCACCCCAA
CATCGTGAAGCTCTTTGAGGTGATTGAGACTGAGAAGACGCTGTACCTGG
TGATGGAGTACGCAAGTGCTGGAGAAGTGTTTGACTACCTCGTGTCGCAT
GGCCGCATGAAGGAGAAGGAAGCTCGAGCCAAGTTCCGACAGATTGTTTC
GGCTGTGCACTATTGTCACCAGAAAAATATTGTACACAGGGACCTGAAGG
CTGAGAACCTCTTGCTGGATGCCGAGGCCAACATCAAGATTGCTGACTTT
GGCTTCAGCAACGAGTTCACGCTGGGATCGAAGCTGGACACGTTCTGCGG
GAGCCCCCCATATGCCGCCCCGGAGCTGTTTCAGGGCAAGAAGTACGACG
GGCCGGAGGTGGACATCTGGAGCCTGGGAGTCATCCTGTACACCCTCGTC
AGCGGCTCCCTGCCCTTCGACGGGCACAACCTCAAGGAGCTGCGGGAGCG
AGTACTCAGAGGGAAGTACCGGGTCCCTTTCTACATGTCAACAGACTGTG
AGAGCATCCTGCGGAGATTTTTGGTGCTGAACCCAGCTAAACGCTGTACT
CTCGAGCAAATCATGAAAGACAAATGGATCAACATCGGCTATGAGGGTGA
GGAGTTGAAGCCATACACAGAGCCCGAGGAGGACTTCGGGGACACCAAGA
GAATTGAGGTGATGGTGGGTATGGGCTACACACGGGAAGAAATCAAAGAG
TCCTTGACCAGCCAGAAGTACAACGAAGTGACCGCCACCTACCTCCTGCT
GGGCAGGAAGACTGAGGAGGGTGGGGACCGGGGCGCCCCAGGGCTGGCCC
TGGCACGGGTGCGGGCGCCCAGCGACACCACCAACGGAACAAGTTCCAGC
AAAGGCACCAGCCACAGCAAAGGGCAGCGGAGTTCCTCTTCCACCTACCA
CCGCCAGCGCAGGCATAGCGATTTCTGTGGCCCATCCCCTGCACCCCTGC
ACCCCAAACGCAGCCCGACGAGCACGGGGGAGGCGGAGCTGAAGGAGGAG
CGGCTGCCAGGCCGGAAGGCGAGCTGCAGCACCGCGGGGAGTGGGAGTCG
AGGGCTGCCCCCCTCCAGCCCCATGGTCAGCAGCGCCCACAACCCCAACA
AGGCAGAGATCCCAGAGCGGCGGAAGGACAGCACGAGCACCCCCAACAAC
CTCCCTCCTAGCATGATGACCCGCAGAAACACCTACGTTTGCACAGAACG
CCCGGGGGCTGAGCGCCCGTCACTGTTGCCAAATGGGAAAGAAAACAGCT
CAGGCACCCCACGGGTGCCCCCTGCCTCCCCCTCCAGTCACAGCCTGGCA
CCCCCATCAGGGGAGCGGAGCCGCCTGGCACGCGGTTCCACCATCCGCAG
CACCTTCCATGGTGGCCAGGTCCGGGACCGGCGGGCAGGGGGTGGGGGTG
GTGGGGGTGTGCAGAATGGGCCCCCTGCCTCTCCCACACTGGCCCATGAG
GCTGCACCCCTGCCCGCCGGGCGGCCCCGCCCCACCACCAACCTCTTCAC
CAAGCTGACCTCCAAACTGACCCGAAGGGTCGCAGACGAACCTGAGAGAA
TCGGGGGACCTGAGGTCACAAGTTGCCATCTACCTTGGGATCAAACGGAA
ACCGCCCCCCGGCTGCTCCGATTCCCCTGGAGTGTGAAGCTGACCAGCTC
GCGCCCTCCTGAGGCCCTGATGGCAGCTCTGCGCCAGGCCACAGCAGCCG
CCCGCTGCCGCTGCCGCCAGCCACAGCCGTTCCTGCTGGCCTGCCTGCAC
GGGGGTGCGGGCGGGCCCGAGCCCCTGTCCCACTTCGAAGTGGAGGTCTG
CCAGCTGCCCCGGCCAGGCTTGCGGGGAGTTCTCTTCCGCCGTGTGGCGG
GCACCGCCCTGGCCTTCCGCACCCTCGTCACCCGCATCTCCAACGACCTC
GAGCTCTGAggatcc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSSSRTVLAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRNSIASCPEEQPHVGNYRLLRTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLVMEYASAGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLLDAEANIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDIWSLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFLVLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGMGYTREEIKESLTSQKYNEVTATYLLLGRKTEEGGDRGAPGLALARVRAPSDTTNGTSSSKGTSHSKGQRSSSSTYHRQRRHSDFCGPSPAPLHPKRSPTSTGEAELKEERLPGRKASCSTAGSGSRGLPPSSPMVSSAHNPNKAEIPERRKDSTSTPNNLPPSMMTRRNTYVCTERPGAERPSLLPNGKENSSGTPRVPPASPSSHSLAPPSGERSRLARGSTIRSTFHGGQVRDRRAGGGGGGGVQNGPPASPTLAHEAAPLPAGRPRPTTNLFTKLTSKLTRRVADEPERIGGPEVTSCHLPWDQTETAPRLLRFPWSVKLTSSRPPEALMAALRQATAAARCRCRQPQPFLLACLHGGAGGPEPLSHFEVEVCQLPRPGLRGVLFRRVAGTALAFRTLVTRISNDLEL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1281
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; Sequence change compared with AK075272 CDS sequence: c1824g, silent in R608.