cDNA Clone BRSK1

• Plasmid: pEBG2T-HA BRSK1 D174A
• Parent Plasmid: pEBG2T
• Expressed: GST-HA-BRSK1 D174A
• Genbank: NM_032430
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

ggatccTACCCATACGATGTGCCAGATTACGCCTCGTCCGGGGCCAAGGAGGGAGGTGGGGGCTCTCCCGCCTACCACCTCCCCCACCCCCACCCCCACCCACCCCAGCACGCCCAATATGTGGGCCCCTATCGGCTGGAGAAGACGCTGGGCAAAGGACAGACAGGGCTGGTTAAACTCGGGGTCCACTGCATCACGGGTCAGAAGGTCGCCATCAAGATCGTGAACCGGGAGAAGCTGTCGGAGTCGGTGCTGATGAAGGTGGAGCGGGAGATCGCCATCCTGAAGCTCATCGAACACCCACATGTCCTCAAGCTCCACGACGTCTACGAGAACAAGAAATATTTGTACCTGGTTCTGGAGCACGTCTCGGGGGGTGAGCTATTCGACTACCTGGTAAAGAAGGGGAGACTGACGCCCAAGGAGGCCCGAAAGTTCTTCCGCCAGATTGTGTCTGCGCTGGACTTCTGCCACAGCTACTCCATCTGCCACAGAGACCTAAAGCCCGAGAACCTGCTTTTGGATGAGAAAAACAACATCCGCATTGCAGcCTTCGGCATGGCGTCCCTGCAGGTGGGGGACAGCCTCCTGGAGACCAGCTGCGGGTCCCCCCATTATGCGTGTCCAGAGGTGATTAAGGGGGAAAAATATGATGGCCGCCGGGCAGACATGTGGAGCTGTGGAGTCATCCTCTTCGCCCTGCTCGTGGGGGCTCTGCCCTTTGATGACGACAACCTCCGCCAGCTGCTGGAGAAGGTGAAACGGGGCGTCTTCCACATGCCCCACTTCATTCCTCCAGATTGCCAGAGCCTCCTGAGGGGAATGATCGAAGTGGAGCCCGAAAAAAGGCTCAGTCTGGAGCAAATTCAGAAACATCCTTGGTACCTAGGCGGGAAACACGAGCCAGACCCGTGCCTGGAGCCAGCCCCTGGCCGCCGGGTAGCCATGCGGAGCCTGCCATCCAACGGAGAGCTGGACCCCGACGTCCTAGAGAGCATGGCATCACTGGGCTGCTTCAGGGACCGCGAGAGGCTGCATCGCGAGCTGCGCAGTGAGGAGGAGAACCAAGAAAAGATGATATATTATCTGCTTTTGGATCGGAAGGAGCGGTATCCCAGCTGTGAGGACCAGGACCTGCCTCCCCGGAATGATGTTGACCCCCCCCGGAAGCGTGTGGATTCTCCCATGCTGAGCCGTCACGGGAAGCGGCGACCAGAGCGGAAGTCCATGGAAGTCCTGAGCATCACCGATGCCGGGGGTGGTGGCTCCCCTGTACCCACCCGACGGGCCTTGGAGATGGCCCAGCACAGCCAGAGATCCCGTAGCGTCAGTGGAGCCTCCACGGGTCTGTCCTCCAGCCCTCTAAGCAGCCCAAGGAGTCCGGTCTTTTCCTTTTCACCGGAGCCGGGGGCTGGAGATGAGGCTCGAGGCGGGGGCTCCCCGACTTCCAAAACGCAGACGCTGCCTTCTCGGGGCCCCAGGGGTGGGGGCGCCGGGGAGCAGCCCCCGCCCCCCAGTGCCCGCTCCACACCCCTGCCCGGCCCCCCAGGCTCCCCGCGCTCCTCTGGCGGGACCCCCTTGCACTCGCCTCTGCACACGCCCCGGGCCAGTCCCACCGGGACCCCGGGGACAACACCACCCCCCAGCCCCGGCGGTGGCGTCGGGGGAGCCGCCTGGAGGAGTCGTCTCAACTCCATCCGCAACAGCTTCCTGGGCTCCCCTCGCTTTCACCGGCGCAAGATGCAGGTCCCTACCGCTGAGGAGATGTCCAGCTTGACGCCAGAGTCCTCCCCGGAGCTGGCAAAACGCTCCTGGTTCGGGAACTTCATCTCCTTGGACAAAGAAGAACAAATATTCCTCGTGCTAAAGGACAAACCTCTCAGCAGCATCAAAGCAGACATCGTCCATGCCTTTCTGTCGATCCCCAGCCTGAGTCACAGTGTGCTGTCACAGACCAGCTTCAGGGCCGAGTACAAGGCCAGTGGCGGCCCCTCCGTCTTCCAAAAGCCCGTCCGCTTCCAGGTGGACATCAGCTCCTCTGAGGGTCCAGAGCCCTCCCCGCGACGGGACGGCAGCGGAGGTGGTGGCATCTACTCCGTCACCTTCACTCTCATCTCGGGTCCCAGCCGTCGGTTCAAGCGAGTGGTGGAGACCATCCAGGCACAGCTCCTGAGCACTCATGACCAGCCCTCCGTGCAGGCCCTGGCAGACGAGAAGAACGGGGCCCAGACCCGGCCTGCTGGTGCCCCACCCCGAAGCCTGCAGCCCCCACCCGGCCGCCCAGACCCAGAGCTGAGCAGCTCTCCCCGCCGAGGCCCCCCCAAGGACAAGAAGCTCCTGGCCACCAACGGGACCCCTCTGCCCTGAAAGCCGAATTCTGCAGATATCCATCACACTGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSTSYPYDVPDYASSGAKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIAAFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGGGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRGPPKDKKLLATNGTPLP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1259
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express BRSK1 D174A with N-terminal GST and HA tags