cDNA Clone AKAP1

• Plasmid: pGEX6P-AKAP1 S151A
• Parent Plasmid: pGEX6P1
• Expressed: GST-AKAP1 S151A
• Genbank: NM_003488*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCAATCCAGTTCCGTTCGCTCTTCCCCTTGGCATTGCCTGG
GATGCTGGCGCTCCTCGGCTGGTGGTGGTTTTTCTCTCGTAAAAAAGGCC
ATGTCAGCAGCCATGATGAGCAGCAGGTGGAGGCTGGTGCTGTGCAGCTG
AGGGCTGACCCTGCCATCAAGGAACCTCTCCCCGTGGAAGACGTCTGTCC
CAAAGTAGTGTCCACACCCCCCAGTGTCACAGAGCCTCCAGAAAAGGAAC
TGTCCACCGTGAGCAAGCTGCCTGCAGAGCCCCCAGCATTGCTCCAGACA
CACCCACCTTGCCGAAGATCAGAGTCCTCGGGCATTCTTCCTAACACCAC
AGACATGAGATTGCGACCAGGAACACGCAGAGATGACAGTACAAAGCTGG
AGCTAGCCCTGACAGGTGGTGAAGCCAAATCGATTCCTCTAGAGTGCCCC
CTTTCAGCCCCAAAGGGTGTACTATTCTCCAGCAAATCAGCTGAGGTGTG
TAAGCAAGATTCCCCCTTCAGCAGGGTGCCAAGGAAGGTCCAGCCAGGCT
ACCCCGTAGTCCCCGCAGAGAAGCGTAGCTCTGGGGAGAGGGCAAGAGAG
ACAGGTGGGGCCGAAGGGACTGGTGATGCCGTGTTGGGGGAAAAGGTGCT
TGAAGAAGCTCTGTTGTCTCGGGAGCATGTCTTGGAATTGGAGAACAGCA
AGGGCCCCAGCCTGGCCTCTTTAGAGGGGGAAGAAGATAAGGGGAAGAGC
AGCTCATCCCAGGTGGTGGGGCCAGTGCAGGAGGAAGAGTATGTAGCAGA
GAAGTTGCCAAGTAGGTTCATCGAGTCGGCTCACACAGAGCTGGCAAAGG
ACGATGCGGCGCCAGCACCCCCAGTCGCAGACGCCAAAGCCCAGGATAGA
GGTGTCGAGGGAGAACTGGGCAATGAGGAGAGCTTGGATAGAAATGAGGA
GGGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAATGAGGAGAGCTTGG
ATAGAAATGAGGAGGGCTTGGATAGAAATGAGGAGATTAAGCGGGCTGCC
TTCCAGATAATCTCCCAAGTGATCTCAGAAGCAACCGAACAGGTGCTGGC
CACCACGGTTGGCAAGGTTGCAGGTCGTGTGTGTCAGGCCAGTCAGCTCC
AAGGGCAGAAGGAAGAGAGCTGTGTCCCAGTTCACCAGAAAACTGTCTTG
GGCCCAGACACTGCGGAGCCTGCCACAGCAGAGGCAGCTGTTGCCCCGCC
GGATGCTGGCCTCCCCTTGCCAGGCCTACCAGCAGAGGGCTCACCACCAC
CAAAGACCTACGTGAGCTGCCTGAAGAGCCTTCTGTCCAGCCCCACCAAG
GACAGTAAGCCAAATATCTCTGCACACCACATCTCCCTGGCCTCCTGCCT
GGCACTGACCACCCCCAGTGAAGAGTTGCCGGACCGGGCAGGCATCCTGG
TGGAAGATGCCACCTGTGTCACCTGCATGTCAGACAGCAGCCAAAGTGTC
CCTTTGGTGGCTTCTCCAGGACACTGCTCAGATTCTTTCAGCACTTCAGG
GCTTGAAGACTCTTGCACAGAGACCAGCTCGAGCCCCAGGGACAAGGCCA
TCACCCCGCCACTGCCAGAAAGTACTGTGCCCTTCAGCAATGGGGTGCTG
AAGGGGGAGTTGTCAGACTTGGGGGCTGAGGATGGATGGACCATGGATGC
GGAAGCAGATCATTCAGGAGGTTCTGACAGGAACAGCATGGATTCCGTGG
ATAGCTGTTGCAGTCTCAAGAAGACTGAGAGCTTCCAAAATGCCCAGGCA
GGCTCCAACCCTAAGAAGGTCGACCTCATCATCTGGGAGATCGAGGTGCC
AAAGCACTTAGTCGGTCGGCTAATTGGCAAGCAGGGGCGCTATGTGAGTT
TTCTGAAGCAAACATCTGGTGCCAAGATCTACATTTCAACCCTGCCTTAC
ACCCAGAGCGTCCAGATCTGCCACATAGAAGGCTCTCAACATCATGTAGA
CAAAGCGCTGAACTTGATTGGGAAGAAGTTCAAAGAGCTGAACCTCACCA
ATATCTACGCTCCCCCATTGCCTTCACTGGCACTGCCTTCTCTGCCGATG
ACATCCTGGCTCATGCTGCCTGATGGCATCACCGTGGAGGTCATTGTGGT
CAACCAGGTCAATGCCGGGCACCTGTTCGTGCAGCAGCACACACACCCTA
CCTTCCACGCGCTGCGCAGCCTCGACCAGCAGATGTACCTCTGTTACTCT
CAGCCTGGAATCCCCACCTTGCCCACCCCAGTGGAAATAACGGTCATCTG
TGCCGCCCCTGGTGCGGACGGGGCCTGGTGGCGAGCCCAAGTGGTTGCCT
CCTACGAGGAGACCAACGAAGTGGAGATTCGATACGTGGACTACGGCGGA
TATAAGAGGGTGAAAGTAGACGTGCTCCGGCAAATCAGGTCTGACTTTGT
CACCCTGCCGTTTCAGGGAGCAGAAGTCCTTCTGGACAGTGTGATGCCCC
TGTCAGACGATGACCAGTTTTCACCGGAAGCAGATGCCGCCATGAGCGAG
ATGACGGGGAATACAGCACTGCTTGCTCAGGTGACAAGTTACAGTCCAAC
TGGTCTTCCTCTGATTCAGCTGTGGAGTGTGGTTGGAGATGAAGTGGTGT
TGATAAACCGGTCCCTGGTGGAGCGAGGCCTTGCCCAGTGGGTAGACAGC
TACTACACAAGCCTTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGGEAKSIPLECPLSAPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQIISQVISEATEQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLPLPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGILVEDATCVTCMSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPESTVPFSNGVLKGELSDLGAEDGWTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGSNPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQSVQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVMPLSDDDQFSPEADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSYYTSL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20011
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for AKAP1 Ser151Ala mutant with N-terminal GST tag (contains preScission protease site); ; Estimated MW of expressed protein : 124151 Daltons