cDNA Clone AKAP1

• Plasmid: pMalP AKAP1 wt
• Parent Plasmid: pMAL6P
• Expressed: MBP-AKAP1
• Genbank: NM_003488
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGGCAATCCAGTTCCGTTCGCTCTTCCCCTTGGCATTGCCTGGGATGCTGGCGCTCCTCGGCTGGTGGTGGTTTTTCTCTCGTAAAAAAGGCCATGTCAGCAGCCATGATGAGCAGCAGGTGGAGGCTGGTGCTGTGCAGCTGAGGGCTGACCCTGCCATCAAGGAACCTCTCCCCGTGGAAGACGTCTGTCCCAAAGTAGTGTCCACACCCCCCAGTGTCACAGAGCCTCCAGAAAAGGAACTGTCCACCGTGAGCAAGCTGCCTGCAGAGCCCCCAGCATTGCTCCAGACACACCCA
CCTTGCCGAAGATCAGAGTCCTCGGGCATTCTTCCTAACACCACAGACAT
GAGATTGCGACCAGGAACACGCAGAGATGACAGTACAAAGCTGGAGCTAG
CCCTGACAGGTGGTGAAGCCAAATCGATTCCTCTAGAGTGCCCCCTTTCA
TCCCCAAAGGGTGTACTATTCTCCAGCAAATCAGCTGAGGTGTGTAAGCA
AGATTCCCCCTTCAGCAGGGTGCCAAGGAAGGTCCAGCCAGGCTACCCCG
TAGTCCCCGCAGAGAAGCGTAGCTCTGGGGAGAGGGCAAGAGAGACAGGT
GGGGCCGAAGGGACTGGTGATGCCGTGTTGGGGGAAAAGGTGCTTGAAGA
AGCTCTGTTGTCTCGGGAGCATGTCTTGGAATTGGAGAACAGCAAGGGCC
CCAGCCTGGCCTCTTTAGAGGGGGAAGAAGATAAGGGGAAGAGCAGCTCA
TCCCAGGTGGTGGGGCCAGTGCAGGAGGAAGAGTATGTAGCAGAGAAGTT
GCCAAGTAGGTTCATCGAGTCGGCTCACACAGAGCTGGCAAAGGACGATG
CGGCGCCAGCACCCCCAGTCGCAGACGCCAAAGCCCAGGATAGAGGTGTC
GAGGGAGAACTGGGCAATGAGGAGAGCTTGGATAGAAATGAGGAGGGCTT
GGATAGAAATGAGGAGGGCTTGGATAGAAATGAGGAGAGCTTGGATAGAA
ATGAGGAGGGCTTGGATAGAAATGAGGAGATTAAGCGGGCTGCCTTCCAG
ATAATCTCCCAAGTGATCTCAGAAGCAACCGAACAGGTGCTGGCCACCAC
GGTTGGCAAGGTTGCAGGTCGTGTGTGTCAGGCCAGTCAGCTCCAAGGGC
AGAAGGAAGAGAGCTGTGTCCCAGTTCACCAGAAAACTGTCTTGGGCCCA
GACACTGCGGAGCCTGCCACAGCAGAGGCAGCTGTTGCCCCGCCGGATGC
TGGCCTCCCCTTGCCAGGCCTACCAGCAGAGGGCTCACCACCACCAAAGA
CCTACGTGAGCTGCCTGAAGAGCCTTCTGTCCAGCCCCACCAAGGACAGT
AAGCCAAATATCTCTGCACACCACATCTCCCTGGCCTCCTGCCTGGCACT
GACCACCCCCAGTGAAGAGTTGCCGGACCGGGCAGGCATCCTGGTGGAAG
ATGCCACCTGTGTCACCTGCATGTCAGACAGCAGCCAAAGTGTCCCTTTG
GTGGCTTCTCCAGGACACTGCTCAGATTCTTTCAGCACTTCAGGGCTTGA
AGACTCTTGCACAGAGACCAGCTCGAGCCCCAGGGACAAGGCCATCACCC
CGCCACTGCCAGAAAGTACTGTGCCCTTCAGCAATGGGGTGCTGAAGGGG
GAGTTGTCAGACTTGGGGGCTGAGGATGGATGGACCATGGATGCGGAAGC
AGATCATTCAGGAGGTTCTGACAGGAACAGCATGGATTCCGTGGATAGCT
GTTGCAGTCTCAAGAAGACTGAGAGCTTCCAAAATGCCCAGGCAGGCTCC
AACCCTAAGAAGGTCGACCTCATCATCTGGGAGATCGAGGTGCCAAAGCA
CTTAGTCGGTCGGCTAATTGGCAAGCAGGGGCGCTATGTGAGTTTTCTGA
AGCAAACATCTGGTGCCAAGATCTACATTTCAACCCTGCCTTACACCCAG
AGCGTCCAGATCTGCCACATAGAAGGCTCTCAACATCATGTAGACAAAGC
GCTGAACTTGATTGGGAAGAAGTTCAAAGAGCTGAACCTCACCAATATCT
ACGCTCCCCCATTGCCTTCACTGGCACTGCCTTCTCTGCCGATGACATCC
TGGCTCATGCTGCCTGATGGCATCACCGTGGAGGTCATTGTGGTCAACCA
GGTCAATGCCGGGCACCTGTTCGTGCAGCAGCACACACACCCTACCTTCC
ACGCGCTGCGCAGCCTCGACCAGCAGATGTACCTCTGTTACTCTCAGCCT
GGAATCCCCACCTTGCCCACCCCAGTGGAAATAACGGTCATCTGTGCCGC
CCCTGGTGCGGACGGGGCCTGGTGGCGAGCCCAAGTGGTTGCCTCCTACG
AGGAGACCAACGAAGTGGAGATTCGATACGTGGACTACGGCGGATATAAG
AGGGTGAAAGTAGACGTGCTCCGGCAAATCAGGTCTGACTTTGTCACCCT
GCCGTTTCAGGGAGCAGAAGTCCTTCTGGACAGTGTGATGCCCCTGTCAG
ACGATGACCAGTTTTCACCGGAAGCAGATGCCGCCATGAGCGAGATGACG
GGGAATACAGCACTGCTTGCTCAGGTGACAAGTTACAGTCCAACTGGTCT
TCCTCTGATTCAGCTGTGGAGTGTGGTTGGAGATGAAGTGGTGTTGATAA
ACCGGTCCCTGGTGGAGCGAGGCCTTGCCCAGTGGGTAGACAGCTACTAC
ACAAGCCTTTGAGCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMAIQFRSLFPLALPGMLALLGW
WWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVSTPP
SVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPG
TRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFS
RVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSR
EHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFI
ESAHTELAKDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEE
GLDRNEESLDRNEEGLDRNEEIKRAAFQIISQVISEATEQVLATTVGKVA
GRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLPLP
GLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSE
ELPDRAGILVEDATCVTCMSDSSQSVPLVASPGHCSDSFSTSGLEDSCTE
TSSSPRDKAITPPLPESTVPFSNGVLKGELSDLGAEDGWTMDAEADHSGG
SDRNSMDSVDSCCSLKKTESFQNAQAGSNPKKVDLIIWEIEVPKHLVGRL
IGKQGRYVSFLKQTSGAKIYISTLPYTQSVQICHIEGSQHHVDKALNLIG
KKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGH
LFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADG
AWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGA
EVLLDSVMPLSDDDQFSPEADAAMSEMTGNTALLAQVTSYSPTGLPLIQL
WSVVGDEVVLINRSLVERGLAQWVDSYYTSL

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20006
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid to express AKAP1 wild type with N-terminal MBP tag (contains preScission protease site); ; Estimated MW of expressed protein : 144084 Daltons