cDNA Clone IKK beta

• Plasmid: pCMV5-FLAG-3 IKK beta
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG IKK beta
• Genbank: AF080158 (gi 14738764)
• Tag: FLAG
• Species: Human

Sequence of Insert

ATGGACTACAAGGACGATGACGATAAGGGGGATCCCCGGAATTCCCGGGT
CGACTCGAGCGGCCGCAGCTGGTCACCTTCCCTGACAACGCAGACATGTG
GGGCCTGGGAAATGAAAGAGCGCCTTGGGACAGGGGGATTTGGAAATGTC
ATCCGATGGCACAATCAGGAAACAGGTGAGCAGATTGCCATCAAGCAGTG
CCGGCAGGAGCTCAGCCCCCGGAACCGAGAGCGGTGGTGCCTGGAGATCC
AGATCATGAGAAGGCTGACCCACCCCAATGTGGTGGCTGCCCGAGATGTC
CCTGAGGGGATGCAGAACTTGGCGCCCAATGACCTGCCCCTGCTGGCCAT
GGAGTACTGCCAAGGAGGAGATCTCCGGAAGTACCTGAACCAGTTTGAGA
ACTGCTGTGGTCTGCGGGAAGGTGCCATCCTCACCTTGCTGAGTGACATT
GCCTCTGCGCTTAGATACCTTCATGAAAACAGAATCATCCATCGGGATCT
AAAGCCAGAAAACATCGTCCTGCAGCAAGGAGAACAGAGGTTAATACACA
AAATTATTGACCTAGGATATGCCAAGGAGCTGGATCAGGGCAGTCTTTGC
ACATCATTCGTGGGGACCCTGCAGTACCTGGCCCCAGAGCTACTGGAGCA
GCAGAAGTACACAGTGACCGTCGACTACTGGAGCTTCGGCACCCTGGCCT
TTGAGTGCATCACGGGCTTCCGGCCCTTCCTCCCCAACTGGCAGCCCGTG
CAGTGGCATTCAAAAGTGCGGCAGAAGAGTGAGGTGGACATTGTTGTTAG
CGAAGACTTGAATGGAACGGTGAAGTTTTCAAGCTCTTTACCCTACCCCA
ATAATCTTAACAGTGTCCTGTCTGAGCGACTGGAGAAGTGGCTGCAACTG
ATGCTGATGTGGCACCCCCGACAGAGGGGCACGGATCCCACGTATGGGCC
CAATGGCTGCTTCAAGGCCCTGGATGACATCTTAAACTTAAAGCTGGTTC
ATATCTTGAACATGGTCACGGGCACCATCCACACCTACCCTGTGACAGAG
GATGAGAGTCTGCAGAGCTTGAAGGCCAGAATCCAACAGGACACGGGCAT
CCCAGAGGAGGACCAGGAGCTGCTGCAGGAAGCGGGCCTGGCGTTGATCC
CCGATAAGCCTGCCACTCAGTGTATTTCAGACGGCAAGTTAAATGAGGGC
CACACATTGGACATGGATCTTGTTTTTCTCTTTGACAACAGTAAAATCAC
CTATGAGACTCAGATCTCCCCACGGCCCCAACCTGAAAGTGTCAGCTGTA
TCCTTCAAGAGCCCAAGAGGAATCTCGCCTTCTTCCAGCTGAGGAAGGTG
TGGGGCCAGGTCTGGCACAGCATCCAGACCCTGAAGGAAGATTGCAACCG
GCTGCAGCAGGGACAGCGAGCCGCCATGATGAATCTCCTCCGAAACAACA
GCTGCCTCTCCAAAATGAAGAATTCCATGGCTTCCATGTCTCAGCAGCTC
AAGGCCAAGTTGGATTTCTTCAAAACCAGCATCCAGATTGACCTGGAGAA
GTACAGCGAGCAAACCGAGTTTGGGATCACATCAGATAAACTGCTGCTGG
CCTGGAGGGAAATGGAGCAGGCTGTGGAGCTCTGTGGGCGGGAGAACGAA
GTGAAACTCCTGGTAGAACGGATGATGGCTCTGCAGACCGACATTGTGGA
CTTACAGAGGAGCCCCATGGGCCGGAAGCAGGGGGGAACGCTGGACGACC
TAGAGGAGCAAGCAAGGGAGCTGTACAGGAGACTAAGGGAAAAACCTCGA
GACCAGCGAACTGAGGGTGACAGTCAGGAAATGGTACGGCTGCTGCTTCA
GGCAATTCAGAGCTTCGAGAAGAAAGTGCGAGTGATCTATACGCAGCTCA
GTAAAACTGTGGTTTGCAAGCAGAAGGCGCTGGAACTGTTGCCCAAGGTG
GAAGAGGTGGTGAGCTTAATGAATGAGGATGAGAAGACTGTTGTCCGGCT
GCAGGAGAAGCGGCAGAAGGAGCTCTGGAATCTCCTGAAGATTGCTTGTA
GCAAGGTCCGTGGTCCTGTCAGTGGAAGCCCGGATAGCATGAATGCCTCT
CGACTTAGCCAGCCTGGGCAGCTGATGTCTCAGCCCTCCACGGCCTCCAA
CAGCTTACCTGAGCCAGCCAAGAAGAGTGAAGAACTGGTGGCTGAAGCAC
ATAACCTCTGCACCCTGCTAGAAAATGCCATACAGGACACTGTGAGGGAA
CAAGACCAGAGTTTCACGGCCCTAGACTGGAGCTGGTTACAGACGGAAGA
AGAAGAGCACAGCTGCCTGGAGCAGGCCTCATGATGTGGGGGGACTCGAC
CCCCTGACATGGGGCAGCCCATAGCAGGCCTTGTGCGGTGGGGGGACTCG
ACCCCCTGACATGGGGCTGCCTGGAGCAGGCCTCATGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDSSGRSWSPSLTTQTCGAWEMKERLGTGGFGNV
IRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDV
PEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDI
ASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLC
TSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPV
QWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLSERLEKWLQL
MLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTE
DESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEG
HTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKV
WGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQL
KAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENE
VKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPR
DQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKV
EEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNAS
RLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVRE
QDQSFTALDWSWLQTEEEEHSCLEQAS

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20292
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 88947.7 Daltons